-
1.发明申请Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开用于磷酸化分子检测和分离的组合物和方法公开(公告)号：US20100009381A1
公开(公告)日：2010-01-14
申请号：US12500854
申请日：2009-07-10
申请人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC分类号： G01N33/53
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。
-
2.发明授权Compositions and methods for detection and isolation of phosphorylated molecules 有权用于磷酸化分子检测和分离的组合物和方法公开(公告)号：US07102005B2
公开(公告)日：2006-09-05
申请号：US10428192
申请日：2003-05-02
申请人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Jixiang Liu , Vladimir Martin , Wayne Patton , Thomas Steinberg
发明人： Brian Agnew , Joseph Beechem , Kyle Gee , Richard Haugland , Jixiang Liu , Vladimir Martin , Wayne Patton , Thomas Steinberg
IPC分类号： C07D239/88 , C07D277/62 , C07D311/82 , C07D417/02 , C07D495/04
CPC分类号： G01N33/50 , G01N33/5008 , G01N33/502 , G01N33/5091 , G01N2333/4709
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。
-
3.发明申请Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开用于磷酸化分子检测和分离的组合物和方法公开(公告)号：US20110021372A1
公开(公告)日：2011-01-27
申请号：US12821003
申请日：2010-06-22
申请人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC分类号： C40B30/04 , C12Q1/68 , G01N33/68 , G01N33/566
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。
-
4.发明申请COMPOSITIONS AND METHODS FOR DETECTION AND ISOLATION OF PHOSPHORYLATED MOLECULES 审中-公开用于检测和分离磷酸化分子的组合物和方法公开(公告)号：US20080044804A1
公开(公告)日：2008-02-21
申请号：US11697103
申请日：2007-04-05
申请人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
发明人： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。
-
公开(公告)号：US20060160068A1
公开(公告)日：2006-07-20
申请号：US10943463
申请日：2004-09-17
申请人： Joseph Beechem , Kyle Gee , David Hagen , Iain Johnson , Hee-Chol Kang , Christina Pastula
发明人： Joseph Beechem , Kyle Gee , David Hagen , Iain Johnson , Hee-Chol Kang , Christina Pastula
IPC分类号： C12Q1/70 , C12Q1/68 , C07K16/46 , C07F5/02 , C07D311/88
CPC分类号： G01N33/542 , C07F9/12 , C07F9/65318 , C07F9/65522 , C07F9/6596 , C07K16/46 , Y10S435/968 , Y10S436/80 , Y10S530/807 , Y10T436/13
摘要： The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample. Alternatively, the ligand analog is fluorogenic wherein the ligand analog is essentially non-fluorescent in solution but when bound by the ligand-binding antibody the detectable signal increases. In this instance a decrease in signal, as opposed to the relieving of quenching, is measured for the presence of a target ligand.
摘要翻译： 本发明提供配体检测试剂，配体类似物和用于测定样品中配体存在的方法。 配体检测试剂包含配体结合抗体和配体类似物以形成抗体 - 配体类似物复合物，其中配体类似物共价键合到报告分子。 该复合物还可以包含与抗体非共价键合以形成三元复合物的标记蛋白质，其中标记蛋白质包含共价键合到标记部分的单价抗体片段或非抗体蛋白质。 取决于报道分子和配体结合抗体，报告分子被配体结合抗体或标记蛋白的标记部分淬灭，其中淬灭量与存在于 样品。 或者，配体类似物是荧光的，其中配体类似物在溶液中基本上是非荧光的，但当被配体结合抗体结合时，可检测信号增加。 在这种情况下，与目标配体的存在相比，测量了与消除淬灭相反的信号降低。
-
公开(公告)号：US08535894B2
公开(公告)日：2013-09-17
申请号：US13610009
申请日：2012-09-11
IPC分类号： G01N33/53
摘要： Provided are labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Discrete subsets of labeling reagent and immune-labeled complexes are provided that facilitate the simultaneous detection of multiple targets in a sample-complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译： 提供标记试剂和用于标记一抗的标记试剂和用于使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中的靶标的方法。 标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和力，并且共价连接到标记物上。 提供标记试剂和免疫标记复合物的离散子集，其有利于同时检测样品中多个靶标的复合物，其特征在于i）标记与标记试剂的比例，或ii）所述标记的物理性质，或iii ）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。 这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。
-
公开(公告)号：US20050069962A1
公开(公告)日：2005-03-31
申请号：US10467550
申请日：2002-10-02
IPC分类号： G01N33/53
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译： 本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。 标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和力，并且共价连接到标记物上。 通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。 本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理 或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。 这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。
-
公开(公告)号：US08304195B2
公开(公告)日：2012-11-06
申请号：US12047131
申请日：2008-03-12
IPC分类号： G01N33/53
摘要： The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
摘要翻译： 本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。 标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和力，并且共价连接到标记物上。 通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。 本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理 或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。 这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。
-
公开(公告)号：US07776533B2
公开(公告)日：2010-08-17
申请号：US11552275
申请日：2006-10-24
申请人： Brian Agnew , Joseph Beechem , Kyle R Gee , Richard P Haugland , Thomas H Steinberg , Wayne F Patton
发明人： Brian Agnew , Joseph Beechem , Kyle R Gee , Richard P Haugland , Thomas H Steinberg , Wayne F Patton
IPC分类号： G01N33/53
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
-
公开(公告)号：US20090161828A1
公开(公告)日：2009-06-25
申请号：US12333191
申请日：2008-12-11
IPC分类号： G01N23/207 , C07K14/00 , C12N9/10 , C12N9/48 , C12N9/16
摘要： Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.
摘要翻译： 提供的系统和方法用于产生通常不适于在体外表达系统中以可溶形式表达的目的蛋白质。 在一些方面，本发明提供了使用体外蛋白质合成系统合成蛋白质的方法，所述体外蛋白质合成系统包括诸如载脂蛋白或含有（“AAHC”）蛋白质的两亲性α螺旋的支架蛋白，其中产生比在 缺少支架蛋白。 支架蛋白质可以提供在与脂质相关的体外蛋白质合成系统中或与脂质不相关。 支架蛋白质可以作为蛋白质本身提供，或者可以由核酸模板编码并与感兴趣的蛋白质共表达。 本发明还提供用于合成可溶形式的蛋白质的组合物和试剂盒，其中组合物和试剂盒包括用于蛋白质表达和分离的细胞提取物。
-
-
-
-
-
-
-
-
-
搜索结果
25 条记录 (耗时 0.044 秒)
