公开(公告)号：US20200291409A1

公开(公告)日：2020-09-17

申请号：US16736683

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash FARHADI ,  Gabrielle H HO ,  Daniel P SAWYER ,  Mikhail SHAPIRO

IPC: C12N15/67 ,  C07K14/195

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US20230357780A1

公开(公告)日：2023-11-09

申请号：US18317915

申请日：2023-05-15

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash FARHADI ,  Gabrielle H HO ,  Daniel P SAWYER ,  Mikhail SHAPIRO ,  Robert C. HURT ,  Mengtong DUAN

IPC: C12N15/67 ,  C07K14/195

CPC classification number: C12N15/67 ,  C07K14/195 ,  C12N2510/02

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.