公开(公告)号：US20180030501A1

公开(公告)日：2018-02-01

申请号：US15663635

申请日：2017-07-28

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Raymond W. BOURDEAU ,  Anupama LAKSHMANAN ,  Arash FARHADI ,  Mikhail G. SHAPIRO ,  Audrey LEE-GOSSELIN

IPC: C12Q1/10 ,  A61K49/22

CPC classification number: C12Q1/10 ,  A61K49/1896 ,  A61K49/223 ,  C07K14/195 ,  C07K14/32 ,  C12N15/70 ,  C12N15/74

Abstract: Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.

公开(公告)号：US20200237346A1

公开(公告)日：2020-07-30

申请号：US16736581

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Daniel P. SAWYER ,  Arash FARHADI ,  Avinoam D. BAR-ZION ,  Mikhail SHAPIRO

IPC: A61B8/08

Abstract: The application of a step function increase in acoustic pressure during ultrasound imaging using gas vesicle contrast, along with capturing successive frames of ultrasound imaging and extracting time-series vectors for pixels of the frames, allows for improved imaging down to even the cell level. Template vectors can be used to implement signal separation of the time-series vectors to improve detection.

公开(公告)号：US20200164095A1

公开(公告)日：2020-05-28

申请号：US16656417

申请日：2019-10-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Anupama LAKSHMANAN ,  Arash FARHADI ,  Suchita P. NETY ,  Raymond W. BOURDEAU ,  Mikhail SHAPIRO

IPC: A61K49/22 ,  A61K49/00 ,  C07K14/195 ,  A61B8/08

Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.

公开(公告)号：US20230357780A1

公开(公告)日：2023-11-09

申请号：US18317915

申请日：2023-05-15

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash FARHADI ,  Gabrielle H HO ,  Daniel P SAWYER ,  Mikhail SHAPIRO ,  Robert C. HURT ,  Mengtong DUAN

IPC: C12N15/67 ,  C07K14/195

CPC classification number: C12N15/67 ,  C07K14/195 ,  C12N2510/02

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US20230277695A1

公开(公告)日：2023-09-07

申请号：US18046881

申请日：2022-10-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Anupama LAKSHMANAN ,  Arash FARHADI ,  Suchita P. NETY ,  Raymond W. BOURDEAU ,  Mikhail SHAPIRO

IPC: A61K49/22 ,  A61B8/08 ,  C07K14/195 ,  A61K49/00

CPC classification number: A61K49/223 ,  A61B8/481 ,  A61K49/0002 ,  A61K49/0045 ,  A61K49/0093 ,  A61K49/221 ,  C07K14/195 ,  A61B8/5238 ,  C07K2319/00

Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.

公开(公告)号：US20210301298A1

公开(公告)日：2021-09-30

申请号：US17334953

申请日：2021-05-31

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Raymond W. BOURDEAU ,  Anupama LAKSHMANAN ,  Arash FARHADI ,  Mikhail G. SHAPIRO ,  Audrey LEE-GOSSELIN

IPC: C12N15/75 ,  C07K14/32

Abstract: Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.

公开(公告)号：US20200291409A1

公开(公告)日：2020-09-17

申请号：US16736683

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash FARHADI ,  Gabrielle H HO ,  Daniel P SAWYER ,  Mikhail SHAPIRO

IPC: C12N15/67 ,  C07K14/195

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US20180038922A1

公开(公告)日：2018-02-08

申请号：US15663600

申请日：2017-07-28

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： George J. LU ,  Mikhail G. SHAPIRO ,  Arash FARHADI ,  Jerzy O. SZABLOWSKI

IPC: G01R33/28 ,  G01R33/50 ,  A61B8/08 ,  A61B5/055 ,  G01R33/48

CPC classification number: G01R33/281 ,  A61B5/055 ,  A61B8/4416 ,  A61B8/481 ,  A61B8/5261 ,  G01R33/4814 ,  G01R33/50 ,  G01R33/5601 ,  G01R33/5602

Abstract: Gas vesicle protein structures and related compositions, methods, and systems for singleplexed and/or multiplexed magnetic resonance imaging of a target site alone or in combination with ultrasound are described, in which the gas vesicle protein structures provide contrast for the imaging.

公开(公告)号：US20180028693A1

公开(公告)日：2018-02-01

申请号：US15613104

申请日：2017-06-02

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Anupama LAKSHMANAN ,  Arash FARHADI ,  Suchita P. NETY ,  Raymond W. BOURDEAU ,  Mikhail SHAPIRO

IPC: A61K49/22 ,  A61B8/08

CPC classification number: A61K49/223 ,  A61B8/481 ,  A61B8/5238 ,  A61K49/0002 ,  A61K49/0045 ,  A61K49/0093 ,  A61K49/221 ,  C07K14/195 ,  C07K2319/00

Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.