公开(公告)号：US20240011021A1

公开(公告)日：2024-01-11

申请号：US18173735

申请日：2023-02-23

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Matthew S. CURTIS ,  Mary ARRASTIA ,  David A. SELCK ,  Mitchell GUTTMAN

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806

CPC classification number: C12N15/1065 ,  B01L3/502715 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806 ,  B01L3/502738 ,  B01L2200/16 ,  B01L2300/161 ,  B01L2300/0861 ,  B01L2200/027 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2400/065 ,  C40B30/04

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

公开(公告)号：US20200179420A1

公开(公告)日：2020-06-11

申请号：US16345666

申请日：2017-10-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell GUTTMAN ,  Chun-Kan CHEN

IPC: A61K31/706 ,  C12N15/01 ,  A61K31/405 ,  A61K31/165 ,  A61K31/473

Abstract: A reactivation composition for activating or re-activating expression of a silenced X chromosome gene in a cell includes a non-cytotoxic histone deacetylase (HDAC) inhibitor. The reactivation composition includes the non-cytotoxic HDAC inhibitor and may further include a DNA methylation inhibitor. A method of activating or re-activating expression of a silenced X chromosome gene in a cell includes administering a reactivation composition including a non-cytotoxic HDAC inhibitor. The method of activating or re-activating expression of a silenced X chromosome gene may further includes administering a reactivation composition that includes a non-cytotoxic HDAC inhibitor and an inhibitor of DNA methylation.

公开(公告)号：US20190144854A1

公开(公告)日：2019-05-16

申请号：US16141901

申请日：2018-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Matthew S. CURTIS ,  Mary ARRASTIA ,  David A. SELCK ,  Mitchell GUTTMAN

IPC: C12N15/10

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.