公开(公告)号：US20240011021A1

公开(公告)日：2024-01-11

申请号：US18173735

申请日：2023-02-23

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Matthew S. CURTIS ,  Mary ARRASTIA ,  David A. SELCK ,  Mitchell GUTTMAN

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806

CPC classification number: C12N15/1065 ,  B01L3/502715 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806 ,  B01L3/502738 ,  B01L2200/16 ,  B01L2300/161 ,  B01L2300/0861 ,  B01L2200/027 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2400/065 ,  C40B30/04

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

公开(公告)号：US20190144854A1

公开(公告)日：2019-05-16

申请号：US16141901

申请日：2018-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Matthew S. CURTIS ,  Mary ARRASTIA ,  David A. SELCK ,  Mitchell GUTTMAN

IPC: C12N15/10

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

公开(公告)号：US20180321137A1

公开(公告)日：2018-11-08

申请号：US15774004

申请日：2016-11-04

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jesus Rodriguez MANZANO ,  Mikhail KARYMOV ,  David A. SELCK ,  Stefano BEGOLO ,  Erik B. JUE ,  Dmitriy V. ZHUKOV

IPC: G01N21/25 ,  G06K9/00 ,  G06T7/90 ,  G01J3/50 ,  G01J3/02 ,  C12Q1/6844

CPC classification number: G01N21/253 ,  C12Q1/6844 ,  G01J3/0272 ,  G01J3/46 ,  G01J3/50 ,  G01N2201/0221 ,  G06K9/00134 ,  G06T7/00 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/30024 ,  C12Q2527/101 ,  C12Q2537/165 ,  C12Q2563/159

Abstract: The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.