公开(公告)号：US11162910B2

公开(公告)日：2021-11-02

申请号：US15194367

申请日：2016-06-27

Applicant: CALIPER LIFE SCIENCES, INC. ,  Canon U.S.A., Inc.

Inventor： Steven A. Sundberg ,  Michael R. Knapp ,  Ivor T. Knight ,  Deborah J. Boles ,  Aaron Rulison ,  Wesley B. Dong ,  Andrew Fabans ,  Edward Donlon ,  Robert Moti ,  Michael Slater

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6862 ,  G01N21/64 ,  G01N25/04 ,  B01L3/00 ,  B01L7/00 ,  G01N21/33

Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

公开(公告)号：US20160377562A1

公开(公告)日：2016-12-29

申请号：US15194367

申请日：2016-06-27

Applicant: CALIPER LIFE SCIENCES, INC. ,  CANON U.S. LIFE SCIENCES, INC.

Inventor： Steven A. Sundberg ,  Michael R. Knapp ,  Ivor T. Knight ,  Deborah J. Boles ,  Aaron Rulison ,  Wesley B. Dong ,  Andrew Fabans ,  Allen Boronkay ,  Edwawrd Donlon ,  Robert Moti ,  Michael Slater

IPC: G01N25/04 ,  G01N21/33 ,  B01L3/00 ,  G01N21/64 ,  C12Q1/68 ,  B01L7/00

CPC classification number: G01N25/04 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1833 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6862 ,  G01N21/33 ,  G01N21/6428 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q2527/101

Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

Abstract translation: 本发明提供了采用微流体技术产生分子熔融曲线的新颖方法和装置。 特别地，根据本发明的装置和方法可用于提供PCR扩增产物的分析。

公开(公告)号：US10814321B2

公开(公告)日：2020-10-27

申请号：US15997501

申请日：2018-06-04

Applicant: Canon U.S.A., Inc.

Inventor： Hiroshi Inoue ,  Ivor T. Knight ,  Gregory A. Dale ,  Rita R. Colwell

IPC: B01L3/02 ,  B01L3/00 ,  G01N21/03 ,  G01N35/00 ,  B01L7/00

Abstract: At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.

公开(公告)号：US11351545B2

公开(公告)日：2022-06-07

申请号：US15664843

申请日：2017-07-31

Applicant: Canon U.S.A., Inc. ,  University of Utah Research Foundation

Inventor： Robert J. Pryor ,  Carl T. Wittwer ,  Scott O. Sundberg ,  Ivor T. Knight ,  Joseph T. Myrick ,  Robert A. Palais ,  Jeanette Y. Paek

IPC: C12Q1/6827 ,  B01L3/00 ,  C12Q1/68 ,  B01L7/00 ,  C12Q1/6806

Abstract: A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50° C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.