公开(公告)号：US20160377562A1

公开(公告)日：2016-12-29

申请号：US15194367

申请日：2016-06-27

申请人： CALIPER LIFE SCIENCES, INC. ,  CANON U.S. LIFE SCIENCES, INC.

发明人： Steven A. Sundberg ,  Michael R. Knapp ,  Ivor T. Knight ,  Deborah J. Boles ,  Aaron Rulison ,  Wesley B. Dong ,  Andrew Fabans ,  Allen Boronkay ,  Edwawrd Donlon ,  Robert Moti ,  Michael Slater

IPC分类号： G01N25/04 ,  G01N21/33 ,  B01L3/00 ,  G01N21/64 ,  C12Q1/68 ,  B01L7/00

CPC分类号： G01N25/04 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1833 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6862 ,  G01N21/33 ,  G01N21/6428 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q2527/101

摘要： The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

摘要翻译： 本发明提供了采用微流体技术产生分子熔融曲线的新颖方法和装置。 特别地，根据本发明的装置和方法可用于提供PCR扩增产物的分析。

公开(公告)号：US09983155B2

公开(公告)日：2018-05-29

申请号：US13840994

申请日：2013-03-15

申请人： Canon U.S. Life Sciences, Inc.

发明人： Ivor T. Knight ,  Deborah John Boles ,  Aaron Rulison ,  Wesley B. Dong ,  Andrew Fabans ,  Allen Boronkay ,  Edward Donlon ,  Robert Moti ,  Michael Slater ,  Steven A. Sundberg ,  Michael R. Knapp

IPC分类号： G01N25/04 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  G01N21/33 ,  G01N21/64

CPC分类号： G01N25/04 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1833 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6862 ,  G01N21/33 ,  G01N21/6428 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q2527/101

摘要： The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

公开(公告)号：USD767782S1

公开(公告)日：2016-09-27

申请号：US29509085

申请日：2014-11-13

申请人： Canon U.S. Life Sciences, Inc.

设计人： Weidong Cao ,  Kenton C. Hasson ,  Ivor T. Knight ,  Takayoshi Hanagata ,  Chris Felice ,  Kazuhiko Hasegawa ,  Ronald Kurz ,  Scott Sundberg ,  Ralph McCann ,  Jason Clemons

公开(公告)号：US20140093879A1

公开(公告)日：2014-04-03

申请号：US13840994

申请日：2013-03-15

申请人： Canon U.S. Life Sciences, Inc.

发明人： Ivor T. Knight ,  Deborah John Boles ,  Aaron Rulison ,  Wesley B. Dong ,  Andrew Fabans ,  Allen Boronkay ,  Edward Donlon ,  Robert Moti ,  Michael Slater

IPC分类号： C12Q1/68

CPC分类号： G01N25/04 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1833 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6862 ,  G01N21/33 ,  G01N21/6428 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q2527/101

摘要： The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

摘要翻译： 本发明提供了采用微流体技术产生分子熔融曲线的新颖方法和装置。 特别地，根据本发明的装置和方法可用于提供PCR扩增产物的分析。

公开(公告)号：US10363558B2

公开(公告)日：2019-07-30

申请号：US14833939

申请日：2015-08-24

申请人： Canon U.S. Life Sciences, Inc.

发明人： Ivor T. Knight ,  Kenton C. Hasson ,  Johnathan S. Coursey ,  Hongye Liang ,  Sami Kanderian ,  Gregory H. Owen ,  Weidong Cao ,  Ying-Xin Wang ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy ,  Eric Schneider ,  Takayoshi Hanagata ,  Hiroshi Inoue ,  Shulin Zeng ,  Brian Bean ,  Franklin Regan

IPC分类号： B01L3/00 ,  B01L7/00 ,  G01N21/77 ,  C12Q1/686 ,  G01N35/08 ,  G01N35/10

摘要： The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

公开(公告)号：US20180311661A1

公开(公告)日：2018-11-01

申请号：US15997501

申请日：2018-06-04

申请人： Canon U.S. Life Sciences, Inc.

发明人： Hiroshi Inoue ,  Ivor T. Knight ,  Gregory A. Dale ,  Rita R. Colwell

IPC分类号： B01L3/02 ,  B01L3/00 ,  G01N21/03 ,  B01L7/00 ,  G01N35/00

摘要： At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.

公开(公告)号：US20150167057A1

公开(公告)日：2015-06-18

申请号：US14331886

申请日：2014-07-15

申请人： Canon U.S. Life Sciences, Inc.

发明人： Ivor T. Knight ,  Deborah J. Boyles

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6813 ,  B01L3/502715 ,  B01L7/525 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2300/1833 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/06

摘要： The present invention provides a method for performing thermal melt analysis using a microfluidic device, the method comprising providing a microfluidic device having at least one microfluidic channel, introducing fluid comprising into the at least one microfluidic channel, continuously flowing the fluid through the at least one microfluidic channel while varying the temperature of the entire fluid stream as it moves through the at least one microfluidic channel by uniformly heating the entire fluid stream, and measuring, while continuously flowing the fluid through the at least one microfluidic channel, a detectable property emanating from the fluid.

摘要翻译： 本发明提供一种使用微流体装置进行热熔融分析的方法，所述方法包括提供具有至少一个微流体通道的微流体装置，将包含至少一个微流体通道的流体引入至少一个流体 微流体通道，同时通过均匀加热整个流体流来改变整个流体流的温度，同时在流体连续流过至少一个微流体通道的过程中测量可检测的特性， 流体。

公开(公告)号：US20180321170A1

公开(公告)日：2018-11-08

申请号：US15990369

申请日：2018-05-25

申请人： Canon U.S. Life Sciences, Inc.

发明人： Ivor T. Knight ,  Deborah John Boles ,  Aaron Rulison ,  Wesley B. Dong ,  Andrew Fabans ,  Allen Boronkay ,  Edward Donlon ,  Robert Moti ,  Michael Slater ,  Steven A. Sundberg ,  Michael R. Knapp

IPC分类号： G01N25/04 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6862 ,  G01N21/33 ,  G01N21/64 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/68

CPC分类号： G01N25/04 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2300/1833 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6862 ,  G01N21/33 ,  G01N21/6428 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q2527/101

摘要： The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

公开(公告)号：US20180111125A1

公开(公告)日：2018-04-26

申请号：US15664843

申请日：2017-07-31

申请人： Canon U.S. Life Sciences, Inc.

发明人： Robert J. Pryor ,  Carl T. Wittwer ,  Scott O. Sundberg ,  Ivor T. Knight ,  Joseph T. Myrick ,  Robert A. Palais ,  Jeanette Y. Paek

IPC分类号： B01L3/00 ,  C12Q1/6806 ,  B01L7/00

CPC分类号： B01L3/502784 ,  B01L3/50851 ,  B01L7/52 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q2527/107 ,  C12Q2565/629

摘要： A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50° C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.

公开(公告)号：US09580744B2

公开(公告)日：2017-02-28

申请号：US14331886

申请日：2014-07-15

申请人： Canon U.S. Life Sciences, Inc.

发明人： Ivor T. Knight ,  Deborah J. Boles

IPC分类号： C12P19/34 ,  C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/6813 ,  B01L3/502715 ,  B01L7/525 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2300/1833 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/06

摘要： The present invention provides a method for performing thermal melt analysis using a microfluidic device, the method comprising providing a microfluidic device having at least one microfluidic channel, introducing fluid comprising into the at least one microfluidic channel, continuously flowing the fluid through the at least one microfluidic channel while varying the temperature of the entire fluid stream as it moves through the at least one microfluidic channel by uniformly heating the entire fluid stream, and measuring, while continuously flowing the fluid through the at least one microfluidic channel, a detectable property emanating from the fluid.

摘要翻译： 本发明提供一种使用微流体装置进行热熔融分析的方法，所述方法包括提供具有至少一个微流体通道的微流体装置，将包含在所述至少一个微流体通道中的流体引入至少一个流体 微流体通道，同时当整个流体流通过均匀加热整个流体流而移动通过至少一个微流体通道时改变整个流体流的温度，并且在使流体连续流过至少一个微流体通道的同时测量可检测的特性， 流体。