公开(公告)号：US09765169B2

公开(公告)日：2017-09-19

申请号：US14785334

申请日：2014-04-17

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Subha Ranjan Das ,  Saadyah Averick ,  Sourav K. Dey ,  Krzysztof Matyjaszewski

IPC: C12Q1/68 ,  C08F251/00 ,  C07H21/00 ,  C07H1/00

CPC classification number: C08F251/00 ,  C07H1/00 ,  C07H21/00

Abstract: A method of synthesizing a polynucleotide composition includes attaching a compound including at least one initiator or at least one transfer agent for a reversible deactivation radical polymerization to an end of a nucleotide chain assembly immobilized upon a solid phase support during a solid phase synthesis of a polynucleotide so that the initiator or the transfer agent is attacked to the end of a nucleotide chain assembly in a manner which is stable under conditions of deprotection of the polynucleotide, and growing a polymer from the initiator of from a site of the chain transfer agent via the reversible deactivation radical polymerization to form the polynucleotide composition.