公开(公告)号：US20180142288A1

公开(公告)日：2018-05-24

申请号：US15802066

申请日：2017-11-02

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Munira F. Fouz ,  Krzysztof Matyjaszewski ,  Bruce A. Armitage ,  Subha Ranjan Das ,  Saadyah Averick

IPC: C12Q1/6834 ,  C07H21/04 ,  C08L57/00

CPC classification number: C12Q1/6834 ,  A61K47/6883 ,  C07H21/04 ,  C08F2438/01 ,  C08L57/00 ,  C08L57/12 ,  C08L2203/02 ,  C08L2205/14 ,  G01N33/582

Abstract: A composition includes a polymer including extending chains, side chains, or branches. One (or more) of a plurality of a first strand of nucleic acid is attached to each of a plurality of the side chains. One (or more) of a plurality of a second strand of nucleic acid, which is complementary to the first strand of nucleic acid, is complexed to each of the plurality of the first strand of nucleic acid to form a double strand of nucleic acid on each of the plurality of the side chains. At least one fluorescent compound is associated with the double strand of nucleic acid on each of the plurality of the side chains.

公开(公告)号：US20150275206A1

公开(公告)日：2015-10-01

申请号：US14418987

申请日：2013-08-01

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Subha Ranjan Das ,  Eduardo Paredes ,  Saadyah Averick ,  Krzysztof Matyjaszewski

IPC: C12N15/113

CPC classification number: C12N15/113 ,  A61K47/58 ,  A61K47/64 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2320/32 ,  C12N2320/51

Abstract: A delivery system for delivering oligonucleotides includes a conjugate includes a complexing agent including an oligonucleotide, a peptide nucleic acid or chimera thereof and a polymer covalently attached to the complexing agent. The complexing agent of the conjugate is adapted to complex a biologically active agent thereto after formation of the conjugate.

Abstract translation: 用于递送寡核苷酸的递送系统包括缀合物，其包括络合剂，其包括寡核苷酸，肽核酸或其嵌合体和共价连接到络合剂的聚合物。 缀合物的络合剂适于在形成缀合物之后将生物活性剂复合到其上。

公开(公告)号：US09765169B2

公开(公告)日：2017-09-19

申请号：US14785334

申请日：2014-04-17

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Subha Ranjan Das ,  Saadyah Averick ,  Sourav K. Dey ,  Krzysztof Matyjaszewski

IPC: C12Q1/68 ,  C08F251/00 ,  C07H21/00 ,  C07H1/00

CPC classification number: C08F251/00 ,  C07H1/00 ,  C07H21/00

Abstract: A method of synthesizing a polynucleotide composition includes attaching a compound including at least one initiator or at least one transfer agent for a reversible deactivation radical polymerization to an end of a nucleotide chain assembly immobilized upon a solid phase support during a solid phase synthesis of a polynucleotide so that the initiator or the transfer agent is attacked to the end of a nucleotide chain assembly in a manner which is stable under conditions of deprotection of the polynucleotide, and growing a polymer from the initiator of from a site of the chain transfer agent via the reversible deactivation radical polymerization to form the polynucleotide composition.

公开(公告)号：US20170158836A1

公开(公告)日：2017-06-08

申请号：US15039219

申请日：2014-11-25

Applicant: Carnegie Mellon University

Inventor： Krzysztof Matyjaszewski ,  Saadyah Averick ,  Hongkun He

IPC: C08J9/26 ,  C02F3/34 ,  C12N9/96 ,  C08J9/00 ,  C08J9/36

CPC classification number: C08J9/26 ,  B01D71/40 ,  B01D71/44 ,  B01D71/52 ,  B01D71/82 ,  B01D2323/30 ,  C02F3/342 ,  C08J9/0061 ,  C08J9/36 ,  C08J2201/0462 ,  C08J2205/022 ,  C08J2205/05 ,  C08J2333/06 ,  C08J2333/12 ,  C08J2335/02 ,  C08J2489/00 ,  C12N9/96 ,  C12Y304/21004

Abstract: A three-dimensionally ordered macroporous hydrogel for immobilizing a selected bioresponsive molecule and method of making are disclosed. The three-dimensionally ordered macroporous hydrogel comprises a crosslinked polymer that has a system of interconnected pores. The interconnected pores have a uniform pore size in the range of 50 to 5000 nm, and a plurality of first pore functional groups. The plurality of first pore functional groups is selected to immobilize a selected bioresponsive molecule. Examples of bioresponsive molecules include an enzyme; a molecule for: a protein scaffold, solid phase synthesis, nucleic acid synthesis, polypeptide synthesis, analyte detection, adsorption of analytes and measuring analyte concentrations, organic synthesis, and degradation of biologically active agents in wastewater. A method includes forming a colloidal crystal template, polymerizing a hydrogel within the pores of the colloidal crystal template, and selectively removing the colloidal crystal template. The hydrogel can be polymerized using CRP, ATRP and FRP polymerization processes.

公开(公告)号：US10982266B2

公开(公告)日：2021-04-20

申请号：US15802066

申请日：2017-11-02

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Munira F. Fouz ,  Krzysztof Matyjaszewski ,  Bruce A. Armitage ,  Subha Ranjan Das ,  Saadyah Averick

IPC: C12Q1/68 ,  C12Q1/6834 ,  C07H21/04 ,  C08L57/00 ,  G01N33/58 ,  C08L57/12 ,  A61K47/68

Abstract: A composition includes a polymer including extending chains, side chains, or branches. One (or more) of a plurality of a first strand of nucleic acid is attached to each of a plurality of the side chains. One (or more) of a plurality of a second strand of nucleic acid, which is complementary to the first strand of nucleic acid, is complexed to each of the plurality of the first strand of nucleic acid to form a double strand of nucleic acid on each of the plurality of the side chains. At least one fluorescent compound is associated with the double strand of nucleic acid on each of the plurality of the side chains.