公开(公告)号：US20180142288A1

公开(公告)日：2018-05-24

申请号：US15802066

申请日：2017-11-02

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Munira F. Fouz ,  Krzysztof Matyjaszewski ,  Bruce A. Armitage ,  Subha Ranjan Das ,  Saadyah Averick

IPC: C12Q1/6834 ,  C07H21/04 ,  C08L57/00

CPC classification number: C12Q1/6834 ,  A61K47/6883 ,  C07H21/04 ,  C08F2438/01 ,  C08L57/00 ,  C08L57/12 ,  C08L2203/02 ,  C08L2205/14 ,  G01N33/582

Abstract: A composition includes a polymer including extending chains, side chains, or branches. One (or more) of a plurality of a first strand of nucleic acid is attached to each of a plurality of the side chains. One (or more) of a plurality of a second strand of nucleic acid, which is complementary to the first strand of nucleic acid, is complexed to each of the plurality of the first strand of nucleic acid to form a double strand of nucleic acid on each of the plurality of the side chains. At least one fluorescent compound is associated with the double strand of nucleic acid on each of the plurality of the side chains.

公开(公告)号：US09765169B2

公开(公告)日：2017-09-19

申请号：US14785334

申请日：2014-04-17

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Subha Ranjan Das ,  Saadyah Averick ,  Sourav K. Dey ,  Krzysztof Matyjaszewski

IPC: C12Q1/68 ,  C08F251/00 ,  C07H21/00 ,  C07H1/00

CPC classification number: C08F251/00 ,  C07H1/00 ,  C07H21/00

Abstract: A method of synthesizing a polynucleotide composition includes attaching a compound including at least one initiator or at least one transfer agent for a reversible deactivation radical polymerization to an end of a nucleotide chain assembly immobilized upon a solid phase support during a solid phase synthesis of a polynucleotide so that the initiator or the transfer agent is attacked to the end of a nucleotide chain assembly in a manner which is stable under conditions of deprotection of the polynucleotide, and growing a polymer from the initiator of from a site of the chain transfer agent via the reversible deactivation radical polymerization to form the polynucleotide composition.

公开(公告)号：US20220145291A1

公开(公告)日：2022-05-12

申请号：US17430837

申请日：2020-02-14

Applicant: Carnegie Mellon University

Inventor： Subha Ranjan Das ,  Phil G. Campbell ,  Krzysztof Matyjaszewski ,  Sushil Lathwal ,  Saigopalakrishna Saileelaprasad Yernei

IPC: C12N15/11 ,  A61K47/54 ,  A61K47/60 ,  A61K47/69

Abstract: Provided herein are tethered extracellular vesicles and methods of making tethered extracellular vesicles.

公开(公告)号：US10982266B2

公开(公告)日：2021-04-20

申请号：US15802066

申请日：2017-11-02

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Munira F. Fouz ,  Krzysztof Matyjaszewski ,  Bruce A. Armitage ,  Subha Ranjan Das ,  Saadyah Averick

IPC: C12Q1/68 ,  C12Q1/6834 ,  C07H21/04 ,  C08L57/00 ,  G01N33/58 ,  C08L57/12 ,  A61K47/68

Abstract: A composition includes a polymer including extending chains, side chains, or branches. One (or more) of a plurality of a first strand of nucleic acid is attached to each of a plurality of the side chains. One (or more) of a plurality of a second strand of nucleic acid, which is complementary to the first strand of nucleic acid, is complexed to each of the plurality of the first strand of nucleic acid to form a double strand of nucleic acid on each of the plurality of the side chains. At least one fluorescent compound is associated with the double strand of nucleic acid on each of the plurality of the side chains.

公开(公告)号：US09546364B2

公开(公告)日：2017-01-17

申请号：US14213164

申请日：2014-03-14

Applicant: Carnegie Mellon University

Inventor： Subha Ranjan Das ,  Eduardo Paredes

IPC: C12N15/113 ,  C12N15/11

CPC classification number: C12N15/111 ,  C12N2310/319 ,  C12N2310/141 ,  C12N2310/532

Abstract: Provided herein are compositions for the modification of gene expression by RNA interference using a microRNA (miRNA) guide strand precursor. Methods of making and using the miRNA guide strand precursor also are provided. The compositions bypass the Drosha-Exportin-Dicer pathway and are resistant to nucleases.

Abstract translation: 本文提供了通过使用微小RNA（miRNA）引导链前体的RNA干扰修饰基因表达的组合物。 还提供了制备和使用miRNA引导链前体的方法。 组合物绕过Drosha-Exportin-Dicer途径并且对核酸酶具有抗性。

公开(公告)号：US20150275206A1

公开(公告)日：2015-10-01

申请号：US14418987

申请日：2013-08-01

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Subha Ranjan Das ,  Eduardo Paredes ,  Saadyah Averick ,  Krzysztof Matyjaszewski

IPC: C12N15/113

CPC classification number: C12N15/113 ,  A61K47/58 ,  A61K47/64 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2320/32 ,  C12N2320/51

Abstract: A delivery system for delivering oligonucleotides includes a conjugate includes a complexing agent including an oligonucleotide, a peptide nucleic acid or chimera thereof and a polymer covalently attached to the complexing agent. The complexing agent of the conjugate is adapted to complex a biologically active agent thereto after formation of the conjugate.

Abstract translation: 用于递送寡核苷酸的递送系统包括缀合物，其包括络合剂，其包括寡核苷酸，肽核酸或其嵌合体和共价连接到络合剂的聚合物。 缀合物的络合剂适于在形成缀合物之后将生物活性剂复合到其上。