公开(公告)号：US20190321490A1

公开(公告)日：2019-10-24

申请号：US16225489

申请日：2018-12-19

Applicant: CELLSCRIPT, LLC

Inventor： Judith Meis ,  Anthony Person ,  Cynthia Chin ,  Jerome Jendrisak ,  Gary Dahl

IPC: A61K48/00 ,  C12P19/34 ,  C12N5/074 ,  C12N15/10 ,  C12N5/0793 ,  A61K33/06 ,  C12P21/02 ,  A61K38/00 ,  C12P19/38 ,  C12N15/87 ,  A61K31/7105 ,  C12N5/077

Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.

公开(公告)号：US11135314B2

公开(公告)日：2021-10-05

申请号：US16225489

申请日：2018-12-19

Applicant: CELLSCRIPT, LLC

Inventor： Judith Meis ,  Anthony Person ,  Cynthia Chin ,  Jerome Jendrisak ,  Gary Dahl

IPC: A61K48/00 ,  A61K38/00 ,  A61K31/7105 ,  A61K33/06 ,  C12N15/10 ,  C12P21/02 ,  C12P19/34 ,  C12N5/074 ,  C12N5/0793 ,  C12N5/077 ,  C12N15/87 ,  C12P19/38

Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.

公开(公告)号：US20140328825A1

公开(公告)日：2014-11-06

申请号：US14368399

申请日：2012-12-31

Applicant: CELLSCRIPT, LLC

Inventor： Judith Meis ,  Anthony Person ,  Cynthia Chin ,  Jerome Jendrisak ,  Gary Dahl

IPC: A61K48/00 ,  C12N5/0793 ,  C12N5/077 ,  C12N15/87 ,  C12P19/38

CPC classification number: A61K48/0066 ,  A61K31/7105 ,  A61K33/06 ,  A61K38/005 ,  A61K48/0041 ,  C12N5/0619 ,  C12N5/0658 ,  C12N5/0696 ,  C12N15/1003 ,  C12N15/1096 ,  C12N15/87 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/73 ,  C12N2506/1307 ,  C12N2510/00 ,  C12P19/34 ,  C12P19/38 ,  C12P21/02 ,  C12Y301/26003 ,  A61K2300/00

Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.

Abstract translation: 本发明涉及用于制备和使用RNA组合物的组合物，试剂盒和方法，所述RNA组合物包含在哺乳动物细胞或生物体中诱导生物或生物化学作用的体外合成的ssRNA，RNA组合物重复或连续引入。 在某些实施方案中，本发明提供用于改变人或其他脊椎动物细胞的分化或表型状态的组合物和方法。 例如，本发明提供了mRNA和方法，用于对表现出第二分化状态或表型的细胞（例如将人体细胞重编程为多能干细胞）展现出第一分化状态或表型的细胞重编程。

公开(公告)号：US20140315988A1

公开(公告)日：2014-10-23

申请号：US14322526

申请日：2014-07-02

Applicant: CELLSCRIPT, LLC

Inventor： Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

IPC: C12N15/85

CPC classification number: C12N5/0696 ,  A61K38/1709 ,  A61K38/1816 ,  A61K38/44 ,  A61K38/465 ,  A61K38/50 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/47 ,  C07K14/4712 ,  C07K14/505 ,  C12N9/0075 ,  C12N9/22 ,  C12N9/78 ,  C12N15/117 ,  C12N15/85 ,  C12N15/87 ,  C12N2310/17 ,  C12N2310/335 ,  C12N2320/30 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02 ,  C12Y114/13039 ,  C12Y301/04012 ,  C12Y305/04004

Abstract: The present invention relates to methods for changing the state of differentiation of a eukaryotic cell, the methods comprising introducing mRNA encoding one or more reprogramming factors into a cell and maintaining the cell under conditions wherein the cell is viable and the mRNA that is introduced into the cell is expressed in sufficient amount and for sufficient time to generate a cell that exhibits a changed state of differentiation compared to the cell into which the mRNA was introduced, and compositions therefor. For example, the present invention provides mRNA molecules and methods for their use to reprogram human somatic cells into pluripotent stem cells.

Abstract translation: 本发明涉及用于改变真核细胞分化状态的方法，所述方法包括将编码一种或多种重编程因子的mRNA引入细胞并将细胞维持在其中细胞存活的条件下，并将导入 表达足够量的细胞并足够的时间产生与引入mRNA的细胞相比表现出改变的分化状态的细胞及其组合物。 例如，本发明提供mRNA分子及其用于将人体细胞重编程为多能干细胞的方法。

公开(公告)号：US12059479B2

公开(公告)日：2024-08-13

申请号：US17463007

申请日：2021-08-31

Applicant: CELLSCRIPT, LLC

Inventor： Judith Meis ,  Anthony Person ,  Cynthia Chin ,  Jerome Jendrisak ,  Gary Dahl

IPC: A61K48/00 ,  A61K31/7105 ,  A61K33/06 ,  A61K38/00 ,  C12N5/074 ,  C12N5/077 ,  C12N5/0793 ,  C12N15/10 ,  C12N15/87 ,  C12P19/34 ,  C12P19/38 ,  C12P21/02

CPC classification number: A61K48/0066 ,  A61K31/7105 ,  A61K33/06 ,  A61K38/005 ,  A61K48/0041 ,  C12N5/0619 ,  C12N5/0658 ,  C12N5/0696 ,  C12N15/1003 ,  C12N15/1096 ,  C12N15/87 ,  C12P19/34 ,  C12P19/38 ,  C12P21/02 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/73 ,  C12N2506/1307 ,  C12N2510/00 ,  C12Y301/26003 ,  A61K33/06 ,  A61K2300/00 ,  A61K31/7105 ,  A61K2300/00

Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.

公开(公告)号：US20220257795A1

公开(公告)日：2022-08-18

申请号：US17463007

申请日：2021-08-31

Applicant: CELLSCRIPT, LLC

Inventor： Judith Meis ,  Anthony Person ,  Cynthia Chin ,  Jerome Jendrisak ,  Gary Dahl

IPC: A61K48/00 ,  C12P21/02 ,  A61K38/00 ,  A61K31/7105 ,  C12P19/34 ,  C12N5/074 ,  C12N15/10 ,  C12N5/0793 ,  A61K33/06 ,  C12N5/077 ,  C12N15/87 ,  C12P19/38

Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.

公开(公告)号：US10201620B2

公开(公告)日：2019-02-12

申请号：US14368399

申请日：2012-12-31

Applicant: CELLSCRIPT, LLC

Inventor： Judith Meis ,  Anthony Person ,  Cynthia Chin ,  Jerome Jendrisak ,  Gary Dahl

IPC: A61K48/00 ,  A61K31/7105 ,  C12N15/87 ,  C12P19/34 ,  C12N5/074 ,  C12N15/10 ,  C12N5/0793 ,  A61K33/06 ,  C12P21/02 ,  C12N5/077 ,  C12P19/38 ,  A61K38/00

Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.