公开(公告)号：US09598456B2

公开(公告)日：2017-03-21

申请号：US14673494

申请日：2015-03-30

Applicant: CEPHEID

Inventor： Alexander A. Gall ,  Sergey G. Lokhov ,  Mikhail A. Podyminogin ,  Ekaterina V. Viazovkina ,  Kevin Patrick Lund

IPC: C07H19/04 ,  C07H21/04 ,  C12Q1/68 ,  C07H19/10 ,  C12N15/11 ,  C07H19/06 ,  C07K5/065

CPC classification number: C07H19/10 ,  C07H19/06 ,  C07K5/06078 ,  C12N15/11 ,  C12N15/111 ,  C12N2310/311 ,  C12N2310/312 ,  C12N2310/3125 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/3181 ,  C12N2310/32 ,  C12N2310/3231 ,  C12N2310/334 ,  C12N2310/3513 ,  C12N2320/52 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/117 ,  C12Q2525/107 ,  C12Q2525/113

Abstract: Disclosed are modified cytosine bases that provide enhanced base-pairing affinity for guanine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified cytosine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.

公开(公告)号：US09598455B2

公开(公告)日：2017-03-21

申请号：US14673403

申请日：2015-03-30

Applicant: CEPHEID

Inventor： Alexander A. Gall ,  Sergey G. Lokhov ,  Mikhail A. Podyminogin ,  Ekaterina V. Viazovkina ,  Kevin Patrick Lund

IPC: C07H19/04 ,  C07H21/04 ,  C12Q1/68 ,  C07H19/06 ,  C07H19/10 ,  C07F9/6574 ,  C07K14/00 ,  C07H19/073

CPC classification number: C07H19/06 ,  C07F9/65744 ,  C07H19/073 ,  C07H19/10 ,  C07H21/00 ,  C07H21/04 ,  C07K14/003 ,  C12Q1/6813 ,  C12Q1/6832 ,  C12Q2525/117

Abstract: Disclosed are modified thymine bases that provide enhanced base-pairing affinity for adenine or 2,6-diaminopurine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified thymine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.

公开(公告)号：US20150275283A1

公开(公告)日：2015-10-01

申请号：US14673494

申请日：2015-03-30

Applicant: CEPHEID

Inventor： Alexander A. GALL ,  Sergey G. Lokhov ,  Mikhail A. Podyminogin ,  Ekaterina V. Viazovkina ,  Kevin Patrick Lund

IPC: C12Q1/68 ,  C07H19/10 ,  C07K5/065 ,  C12N15/11 ,  C07H19/06

CPC classification number: C07H19/10 ,  C07H19/06 ,  C07K5/06078 ,  C12N15/11 ,  C12N15/111 ,  C12N2310/311 ,  C12N2310/312 ,  C12N2310/3125 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/3181 ,  C12N2310/32 ,  C12N2310/3231 ,  C12N2310/334 ,  C12N2310/3513 ,  C12N2320/52 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/117 ,  C12Q2525/107 ,  C12Q2525/113

Abstract: Disclosed are modified cytosine bases that provide enhanced base-pairing affinity for guanine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified cytosine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.

Abstract translation: 公开了修饰的胞嘧啶碱基，其在多核苷酸杂交复合物中对鸟嘌呤碱基提供增强的碱基配对亲和力。 还公开了包含这种修饰的胞嘧啶碱基的多核苷酸寡聚体，多核苷酸杂交复合物。 还公开了各种使用方法。 例如，在一些实施方案中，本文公开的修饰的多核苷酸寡聚体可用作核酸扩增和/或检测的引物和探针。