公开(公告)号：US20230204495A1

公开(公告)日：2023-06-29

申请号：US17996581

申请日：2021-04-27

Applicant: CYTIVA SWEDEN AB

Inventor： Hanno Ehring ,  David Gronowitz ,  Lars Rosengren

IPC: G01N21/27 ,  G01N21/31

CPC classification number: G01N21/274 ,  G01N21/31

Abstract: The present invention relates to computer implemented method performed by a computer (110) configured to calibrate an apparatus for measuring the absorbance of light of a substance (131) for a chromatography system (100), the apparatus (131) comprising a conduit (C) for enabling a fluid to be measured, a light emitter (LE) configured to emit light along an optical path (OP) to a light sensor (S) configured to measure the emitted light, the optical path intersecting the conduit (C), a rotating disc (D) having one or more optical filters, each of the one or more optical filters is arranged with its center passing through the optical path (OP) when rotating, an actuator configured to rotate the disc (D) dependent on a control signal, to the method comprising controlling rotation of (710) the disc (D) to a first position where a first filter, of the one or more optical filters, intersects the optical path (OP) at a first point (P1), measuring (720) a first light absorption value, controlling rotation of (730) the disc (D) to a second position, different to the first position, where the first filter still intersects the optical path (OP) at a second point (P2), measuring (740) a second light absorption value, generating (750) an aggregated light absorption value, calibrating (760) the apparatus (131) by comparing the aggregated light absorption value to a reference light absorption value.

公开(公告)号：US12135276B2

公开(公告)日：2024-11-05

申请号：US16464778

申请日：2017-11-30

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring ,  Mats Rimmo

IPC: G01N21/01 ,  G02B6/36

Abstract: An optical measuring device (1; 1′; 1″; 41; 61) configured for being connected in a flow path, said optical measuring device (1; 1′; 1″; 41; 61) comprising:—a first and a second flow path connector (5, 7; 5′, 7′; 5″, 7″);—at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) provided in between the first and second flow path connectors (5, 7; 5′, 7′; 5″, 7″) such that a flow in a flow path in which the optical measuring device (1; 1′; 1″; 41; 61) can be arranged will flow through the first flow path connector (5; 5′; 5″), the at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) and through the second flow path connector (7; 7′; 7″); and—at least one releasable connection device (9) arranged for releasably connecting the at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) in between the first and second flow path connectors (5, 7; 5′, 7′; 5″, 7″).

公开(公告)号：US12066375B2

公开(公告)日：2024-08-20

申请号：US16464759

申请日：2017-11-30

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring ,  Mats Lundkvist ,  David Carl Martin Bergman ,  Andreas Marcstrom

IPC: G01N21/05 ,  G01N21/03 ,  G01N21/85

CPC classification number: G01N21/05 ,  G01N21/03 ,  G01N21/0303 ,  G01N21/85 ,  G01N2201/0668 ,  G01N2201/08

Abstract: The invention relates to an optical flow cell comprising a housing forming an enclosed and elongated fluid channel arranged along a first axis, an inlet arranged to connect a first outer surface area of the housing to a first end of the fluid channel and an outlet arranged to connect a second outer surface area to a second end of the fluid channel, a first light guide and a second light guide concentrically arranged along a second axis and on opposite side walls of the fluid channel. The invention further relates to a corresponding method to produce an optical flow cell.

公开(公告)号：US11280722B2

公开(公告)日：2022-03-22

申请号：US17056543

申请日：2019-05-29

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring ,  Mats Lundkvist ,  David Carl Martin Bergman ,  Andreas Marcstrom

IPC: G01N21/00 ,  G01N21/05 ,  G01N21/03 ,  G01N21/85

Abstract: Disclosed is an optical flow cell (300′) comprising: a housing (910) forming; an enclosed and elongated fluid channel (920) arranged along a first axis (923); a first light guide (961) and a second light guide (962) generally concentrically arranged along a second axis (970) and on opposite side walls of the fluid channel, said first and second light guides having ends (961c,962c) removed in situ to provide a sensing gap (d).

公开(公告)号：US20240369472A1

公开(公告)日：2024-11-07

申请号：US18775959

申请日：2024-07-17

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring ,  Mats Lundkvist ,  David Carl Martin Bergman ,  Andreas Marcstrom

IPC: G01N21/05 ,  G01N21/03 ,  G01N21/85

Abstract: The invention relates to an optical flow cell comprising a housing forming an enclosed and elongated fluid channel arranged along a first axis, an inlet arranged to connect a first outer surface area of the housing to a first end of the fluid channel and an outlet arranged to connect a second outer surface area to a second end of the fluid channel, a first light guide and a second light guide concentrically arranged along a second axis and on opposite side walls of the fluid channel. The invention further relates to a corresponding method to produce an optical flow cell.

公开(公告)号：US20230101434A1

公开(公告)日：2023-03-30

申请号：US18061854

申请日：2022-12-05

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring

IPC: G01N21/03 ,  G01N21/05 ,  G01N21/33

Abstract: An apparatus for measuring the absorbance of a substance in a solution includes at least one sample cell arranged to contain the solution that is at least partially transparent to light of a predefined wavelength spectrum, at least two light passages through the at least one sample cell, each of the light passages having a known path length, an LED light source arrangement including at least two LEDs, each arranged to emit a light output with a wavelength within the predefined wavelength spectrum. A plurality of optical fibers, one for each light passage, is arranged at each LED for receiving the light output and guiding it to the light passages. A method for measuring the absorbance of a substance in a solution includes providing the LED light source arrangement with an associate fiber bundle for each LED.

公开(公告)号：US10816459B2

公开(公告)日：2020-10-27

申请号：US16130100

申请日：2018-09-13

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring

IPC: G01N21/33 ,  G01N21/05 ,  G01N21/59 ,  A61L2/08 ,  G01N30/74 ,  G01N21/03 ,  G01N33/487

Abstract: The present invention relates to a flow cell (10) comprising a fluid inlet (16) and a fluid outlet (18) separated by a sample flow-through chamber (12) comprising at least one UV-transparent window (22′), wherein the at least one UV-transparent window (22′) is made of a polymer material and has been subjected to Gamma radiation sterilisation. In one aspect, the flow cell is combustible.

公开(公告)号：US12209235B2

公开(公告)日：2025-01-28

申请号：US16328431

申请日：2017-08-28

Applicant: CYTIVA SWEDEN AB

Inventor： Klaus Gebauer ,  Pierre Le Greves ,  Andrew David Pris ,  Jozsef Vasi ,  Sam Petrocelli ,  Hanno Ehring ,  Esmaeil Heidari ,  Ali Can

IPC: C12M1/21 ,  C12M1/00 ,  C12M1/36

Abstract: A device and method for detecting the level of foam in a reactor vessel monitors the intensity of the light or the movement of the light detected by a camera which monitors at least one light source positioned inside the reactor vessel or viewable through the reactor vessel.

公开(公告)号：US11543344B2

公开(公告)日：2023-01-03

申请号：US16078727

申请日：2017-02-27

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring

IPC: G01N21/03 ,  G01N21/05 ,  G01N21/33 ,  G01N21/31

Abstract: An apparatus for measuring the absorbance of a substance in a solution, includes at least one sample cell arranged to contain the solution that is at least partially transparent to light of a predefined wavelength spectrum, at least two light passages through the at least one sample cell, each of the light passages having a known path length, an LED light source arrangement including at least two LEDs, each arranged to emit a light output with a wavelength within the predefined wavelength spectrum. A plurality of optical fibers, one for each light passage, is arranged at each LED for receiving the light output and guiding it to the light passages. A method for measuring the absorbance of a substance in a solution includes providing the LED light source arrangement with an associate fiber bundle for each LED.

公开(公告)号：US20220265877A1

公开(公告)日：2022-08-25

申请号：US17620617

申请日：2020-06-17

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring

IPC: A61L2/10 ,  A61L2/26

Abstract: Disclosed is an ultra-violet (UV) light sterilisable fluid-guiding element (100, 200, 300, 400, 500) configurable to form a part of a normally closed bioprocessing fluid system, at least a portion of the element being formed from a material which is transmissive to UV light, said at least one portion of the element including one or more surfaces (134) configured to contact and guide fluids within the closed system. The element further includes at least one UV light emitting diode (LED) (154) mounted in, on, or adjacent the at least one portion and has sufficient light output to sterilise at least the one or more surfaces (134).