公开(公告)号：US20250067258A1

公开(公告)日：2025-02-27

申请号：US18847659

申请日：2023-04-12

Applicant: CYTIVA SWEDEN AB

Inventor： Andreas Lundin ,  Christopher Ford ,  Klaus Gebauer ,  Per Stromberg

IPC: F04B43/073 ,  F04B43/00 ,  F04B53/16

Abstract: The present disclosure relates to a pump (100, 1100, 2100) for providing substantially continuous liquid flow in a bioprocessing system (200), the pump (100, 1100, 2100) comprising: a pressurisable outlet chamber (122, 1122) in fluidic communication with an outlet (126, 1126, 2126) of the pump (100, 1100, 2100); and a pressurisable feed chamber (120, 1120) connected in series with the outlet chamber (122, 1122), wherein the feed chamber (120, 1120) is configured to fill the outlet chamber (122, 1122) during discharge of liquid from the outlet chamber (122, 1122).

公开(公告)号：US11927503B2

公开(公告)日：2024-03-12

申请号：US17276981

申请日：2019-09-09

Applicant: Cytiva Sweden AB

Inventor： Sameera Simha Hindupur ,  Manoj Kumar Ramakrishna ,  Andreas Castan ,  Swapnil Puranik ,  Klaus Gebauer

IPC: G01M3/28 ,  B29C65/00 ,  B29C65/82 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34

CPC classification number: G01M3/2884 ,  B29C65/8246 ,  B29C66/522 ,  C12M23/40 ,  C12M37/04 ,  C12M41/40

Abstract: The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding (710) a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing (720) an opposite end of one of the conduits, generating (730) a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring (740) a fluid pressure within at least one of the conduits, determining (750) a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining (760) the weld integrity test result as pass or as fail based on the fluid pressure change.

公开(公告)号：US11648490B2

公开(公告)日：2023-05-16

申请号：US17113311

申请日：2020-12-07

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Bjorn Markus Olovsson

IPC: B01D15/18 ,  G01N30/20 ,  G01N30/42 ,  G01N30/46 ,  G01N30/88

CPC classification number: B01D15/1842 ,  G01N30/20 ,  G01N30/461 ,  G01N30/466 ,  G01N30/467 ,  G01N30/88 ,  G01N30/42 ,  G01N30/468 ,  G01N2030/205 ,  G01N2030/8881

Abstract: Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

公开(公告)号：US20230014015A1

公开(公告)日：2023-01-19

申请号：US17777590

申请日：2020-12-11

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer

IPC: G05D11/13 ,  G01N30/34

Abstract: A bioprocess fluid mixing system (3; 3′; 3″; 103; 103′), said fluid mixing system (3; 3′; 3″; 103; 103′) comprising: —at least two fluid inlets (5a, 5b, 5c, 5d, 5e), configured for providing a first fluid into the fluid mixing system through a first fluid inlet (5a) and for providing a second fluid into the fluid mixing system through a second fluid inlet (5b); —at least one valve arrangement (13a, 13b, 13c, 13a′), where a first valve arrangement (13a; 13a′) is in fluid communication with at least both the first fluid inlet (5a) and the second fluid inlet (5b); —at least two pumps (11a, 11b, 11c, 11d, 11e), where a first pump (11a) is in selective fluid communication with at least both the first and the second fluid inlets (5a, 5b) via the first valve arrangement (13a; 13a′) and a second pump (11b) is in fluid communication with at least one of the first and second fluid inlet (5b); and —a common fluid outlet (14) which is in fluid communication with both an outlet (15a) of the first pump (11a) and an outlet (15b) of the second pump (11b), wherein pump rates of the at least two pumps (11a, 11b) and valve positions in the at least one valve arrangement (13a; 13a′; 13b, 13c) are configured to be controllable by a control system (21) such that mixing of at least a first fluid from the first fluid inlet (5a) and a second fluid from the second fluid inlet (5b) can be performed to a requested mixing of the at least two fluids and to a requested combined fluid flow rate at the common fluid outlet (14).

公开(公告)号：US10946183B2

公开(公告)日：2021-03-16

申请号：US15625401

申请日：2017-06-16

Applicant: Cytiva Sweden AB

Inventor： Eric Faldt ,  Klaus Gebauer

IPC: A61M39/10 ,  A61M39/16 ,  F16L39/00 ,  A61M39/18

Abstract: The invention relates to a An aseptic connector comprising a first connection unit (102; 202; 302; 402; 502) provided with a first terminal end surface (104; 204; 304; 404; 504) and a second connection unit (106; 206; 306; 406; 506) provided with a second terminal end surface (108; 208; 308; 408; 508); said first and second terminal end surfaces are each provided with at least one first and second opening (110, 112; 210, 212; 310, 312; 410, 412; 510, 512), which openings are orientated to substantially coincide when said first and second connection units are connected to each other; said first and second openings are sealed by at least one film (114; 214; 314; 414; 514) arranged on said first and second connection units, so that the contact between the film and each connection unit is aseptic; said at least one film is adapted to be mated with a corresponding film on the other connection unit when said first and second connection units are connected to each other; and said mated films are adapted to be pulled out together two and two after mating such that corresponding first and second openings in said first and second terminal end surfaces are mated aseptically. At least two first ports (116; 216; 316; 416; 516) are arranged on the first connection unit, which first ports have fluid connection with the at least one first opening (110; 210; 310; 410; 510) in the first terminal end surface; and in that at least one second port (118; 218; 318; 418; 518) is arranged on the second connection unit, which the at least one second port has a fluid connection with the at least one second opening (112; 212; 312; 412; 512) in the second terminal end surface.

公开(公告)号：US12179195B2

公开(公告)日：2024-12-31

申请号：US17281727

申请日：2019-10-16

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer

IPC: B01L3/00

Abstract: The present invention relates to a bioprocessing fluid sensor arrangement (100) for sensing fluidic properties in a process fluid path with a sensor (S), configured for aseptically connecting the sensor with at least one conditioning fluid while separating said sensor from the process fluid to at least one conditioning fluid e.g. for calibration, cleaning, regenerating and/or storing the sensor, the arrangement comprising a process fluid path (PF) having a process fluid inlet (PI) and a process fluid outlet (PO); a sensor (S) arranged in the process fluid path (PF); a bypass fluid path (BF) in the process fluid path (PF), for bypassing the sensor (S); a conditioning or cleaning fluid path (CF) having an inlet (CI) and an outlet (CO) each aseptically and fluidically connected to the process fluid path (PF), one on each side of the sensor (S); and flow controls (FC) for controlling the flow of fluids, whereby fluids can be controlled to flow either in the process fluid path (PF) via the sensor (S), or in the bypass fluid path (BF) omitting the sensor from the fluid path (PF), or in the conditioning or cleaning fluid path (CF) including the sensor in said flow but omitting the remaining process fluid path (PF) and bypass fluid path (BF).

公开(公告)号：US20230417332A1

公开(公告)日：2023-12-28

申请号：US18462316

申请日：2023-09-06

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer

IPC: F16K7/04 ,  F16K7/12 ,  F16K31/12 ,  F16K31/122 ,  F16K31/126

CPC classification number: F16K7/04 ,  F16K7/12 ,  F16K31/126 ,  F16K31/122 ,  F16K31/12

Abstract: A valve configured to be in a first state, which is an open state when a flow through the tube should be allowed; a second state during some parts of a process run in the system, said second state is a closed state were a first closing force is provided; and a third state during some parts of a process run in the system, said third state is a closed state were a second closing force is provided which is a higher force than the first closing force.

公开(公告)号：US11773358B2

公开(公告)日：2023-10-03

申请号：US16400008

申请日：2019-04-30

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Eva Lindskog ,  Lars Magnusson

IPC: C12M1/00 ,  C12M1/06 ,  C12M1/36

CPC classification number: C12M23/14 ,  C12M27/02 ,  C12M41/48

Abstract: A method for controlling at least one culture parameter in a bioreactor bag (1; 31 a, 31 b) provided in a bioreactor system, the method comprising the steps of:

providing bioreactor information to a control unit (5; 35) controlling the bioreactor system;
controlling the at least one culture parameter in dependence of the bioreactor information.

公开(公告)号：US20220033750A1

公开(公告)日：2022-02-03

申请号：US17296726

申请日：2019-12-10

Applicant: Cytiva Sweden AB

Inventor： Andreas Lundin ,  Bjorn Olovsson ,  Klaus Gebauer ,  Tim Francois ,  Kerstin Erickson

IPC: C12M1/00 ,  C12M1/34

Abstract: A flow distribution device for bioprocess systems, comprising: a flow distribution manifold (12; 112) comprising: at least four fluid connection conduits (14), wherein each fluid connection conduit (14) comprises a first end (18) for fluid connection and an opposite second end (20), and wherein at least three of the fluid connection conduits comprise a membrane (19a) and a valve seat (19b), which membrane (19a) can be put in at least two different positions in relation to the valve seat (19b) for allowing or preventing fluid flow between the first end (18) and the second end (20) of the fluid connection conduit (14); and a central common compartment (30) to which the second ends (20) of each of the fluid connection conduits (14) are connected, whereby the first ends (18) of each of the fluid connection conduits (14) can be in fluid communication with the central common compartment (30) and wherein the fluid connection conduits (14) are entering the central common compartment (30) from at least three different directions; wherein said flow distribution device (10) further comprises at least three membrane actuation members (41) which are provided in connection with one membrane (19a) of the flow distribution manifold (12; 112) each, wherein each of said membrane actuation members (41) is configured for actuating the membrane (19a) to be in at least two different positions in relation to the valve seat (19b), wherein a first position of the membrane (19a) allows flow between the first end (18) and the second end (20) of the fluid connection conduit (14) and a second position of the membrane (19a) prevents flow between the first end (18) and the second end (20) of the fluid connection conduit (14).

公开(公告)号：US20220032301A1

公开(公告)日：2022-02-03

申请号：US17277046

申请日：2019-09-27

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Markus Pitkanen

IPC: B01L3/00

Abstract: The present disclosure relates to a biological fluid processing system and method. The system comprises a fluid processing device comprising at least one fluid path, a pump for providing a pressure in the at least one fluid path, a valve arranged along said fluid path and a first actuator arranged to control the valve to assume a desired opening state of said fluid path. The biological fluid processing system comprises further a processing interface comprising a pump drive for driving the pump of the fluid processing device, and a processing control element comprising a pump control system arranged to control at least the pump drive and a valve control system arranged to control the first actuator. The system is modular. The fluid processing device is comprised in a fluid processing device module having a predetermined fluid processing device configuration. The processing interfaces have a predetermined processing interface configuration. The processing control element is arranged to receive information relating to the predetermined processing interface configuration of the processing interface module and/or the predetermined fluid processing device configuration of the fluid processing device module and control the at least one pump drive and/or the valve based on the received information relating to the predetermined fluid processing device configuration and the predetermined processing interface configuration.