公开(公告)号：US11643627B2

公开(公告)日：2023-05-09

申请号：US16474626

申请日：2017-12-27

Applicant: CYTIVA SWEDEN AB

Inventor： Klaus Gebauer ,  Nagaraj Raghavendra Rao ,  Anindya Kanti De ,  Anindya Sengupta ,  Mats Olsson ,  Colin R. Tuohey ,  Jonathan A. Kenney ,  Richard Lee Damren ,  Ralph Stankowski ,  Nirmal Kumar Thanikachalam

IPC: C12M1/00 ,  C12M3/00

CPC classification number: C12M23/14 ,  C12M23/26 ,  C12M23/28 ,  C12M23/48

Abstract: A support housing for a flexible bioprocess bag, the housing comprising at least one side wall having a first segment and a second segment, the first segment movable in relation to the second segment between an open and a closed position, wherein at least a supporting part of the housing is translatable from an operating position to a bag loading position, wherein the supporting part comprises one or more retainers for holding the flexible bioprocess bag upon re-translation of the supporting part to the operating position and movement of the first segment to the closed position, and wherein the first segment comprises a first drive unit for connecting and driving a first mixing unit in the flexible bioprocess bag.

公开(公告)号：US20220403318A1

公开(公告)日：2022-12-22

申请号：US17763860

申请日：2020-10-01

Applicant: Cytiva Sweden AB

Inventor： Shivakumar Selvaraj ,  Bineesh Kandoth ,  Sahebagouda Alagur ,  Nagaraj Raghavendra Rao ,  Thomas Falkman

IPC: C12M1/36 ,  C12M1/34 ,  C12M1/00

Abstract: The present subject matter discloses a bioreactor apparatus. The bioreactor apparatus comprises a bioreactor vessel configured to culture cells. The bioreactor apparatus furthermore comprises a sensor configured to measure Dissolved Oxygen (DO) in the bioreactor vessel. The DO measurements comprise a plurality of DO values recorded at, at least, a plurality of time instances during operation of the bioreactor apparatus. The bioreactor apparatus furthermore comprises a controller configured to obtain the DO measurements. The controller furthermore is to determine, in real-time or approximately real-time, an oxygen mass transfer co-efficient (kLa) associated with the operation of the bioreactor apparatus. Furthermore, the controller is configured to control, in real-time or approximately real-time, at least one cell culture parameter associated with the operation of the bioreactor apparatus based on the kLa.