公开(公告)号：US12169034B2

公开(公告)日：2024-12-17

申请号：US16077779

申请日：2017-02-21

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Sudheer Mygapula ,  Nachiket Karmarkar ,  Sasikumar Arumugam ,  Bashkar Hv ,  Ajit S. Vernekar ,  Nityananda Maiya ,  Prashanth Hosabettu Mohan ,  Sasi Kumar Nutalapati ,  Sameera Simha Hindupur ,  Shashidhar Hedge

IPC: F16K7/06 ,  B01D61/12 ,  B01D61/22 ,  F16K7/07 ,  G05D16/20

Abstract: The invention discloses a method of controlling a transmembrane pressure (TMP) in a crossflow filtration apparatus, comprising the steps of: a) providing a crossflow filtration apparatus comprising a pump (21) fluidically connected via a retentate compartment (33) of a crossflow filter (32) and a length of flexible tubing to a regulator valve (1; 101) acting on the flexible tubing, wherein the regulator valve is a high precision pinch valve. The apparatus further comprises a first pressure transducer (22) adapted to measure a pressure between the retentate compartment and the regulator valve, a second pressure transducer (39) adapted to measure a pressure between the pump and the retentate compartment and a third pressure transducer (40) adapted to measure a pressure at a permeate outlet (36) of the crossflow filter, and a control unit (23) electrically or electromagnetically connected to said regulator valve, the first, second and third pressure transducers and optionally to the pump; b) pumping a liquid with the pump via the second pressure transducer, through the retentate compartment via the length of flexible tubing, the first pressure transducer and the regulator valve; c) controlling the regulator valve with the control unit, such that a TMP calculated by formula (I) TMP=(Pinlet+Poutlet)/2−Pperm corresponds to a predetermined TMP value or to an interval between two predetermined TMP values, wherein Pinlet is a pressure reading from the second pressure transducer, Poutlet is a pressure reading from the first pressure transducer and Pperm is a pressure reading from the third pressure transducer.

公开(公告)号：US11110398B2

公开(公告)日：2021-09-07

申请号：US15750218

申请日：2016-08-17

Applicant: Cytiva Sweden AB

Inventor： Sasi Kumar Nutalapati ,  Klaus Gebauer ,  Karl Axel Jakob Liderfelt ,  Nachiket Karmarkar ,  Ajit S. Vernekar ,  Amit Kumar Sharma ,  Fredrik Oskar Lundstrom ,  Sangeetha Raghu Ramachandran

IPC: C07K1/34 ,  B01D61/18 ,  B01D61/20 ,  B01D61/22 ,  B01D61/08 ,  B01D61/10 ,  B01D61/12 ,  B01D65/02

Abstract: The disclosure generally relates to methods and apparatus for the efficient quantitative recovery of valuable biological fluids from filtration systems, more particularly to efficient quantitative recovery of valuable biological fluids from high precision separation systems suitable for use in the pharmaceutical and biotechnology industries.

公开(公告)号：US11466241B2

公开(公告)日：2022-10-11

申请号：US15781217

申请日：2016-11-25

Applicant: CYTIVA SWEDEN AB

Inventor： Amit Kumar Singh ,  Klaus Gebauer ,  Supti Datta ,  Jakob Liderfelt ,  Nachiket Karmarkar ,  Ajit S. Vernekar ,  Mats Olsson ,  Rajan Thiyaga ,  Amit K. Sharma ,  Sasi Kumar Nutalapati ,  Fredrik Lundstrom ,  Jean Pierre Neff

IPC: C12M1/00 ,  B01D15/08 ,  B01D61/18 ,  C12M1/34

Abstract: The invention discloses a first unit (1) for treatment of a bioprocess liquid comprising a first lateral face (2), a second lateral face (3) and a front face (4) which meets the two said lateral faces. The front face comprises: a plurality of valves (7) adapted to receive and act upon one or more legs (8) of a disposable flow path (6); optionally one or more pumps (10) adapted to receive and act upon one or more legs of the disposable flow path; optionally one or more sensors (11) adapted to receive and to measure one or more parameters in one or more legs of the disposable flow path; wherein the plurality of valves and optional pumps and sensors are vertically offset from each other to give one or more legs of a disposable flow path received by said valves and optional pumps and sensors a slope of at least 3.0 degrees from the horizontal plane (h).