公开(公告)号：US06297061B1

公开(公告)日：2001-10-02

申请号：US09501732

申请日：2000-02-10

申请人： Caicai Wu ,  Bernhard Weigl ,  Margaret A. Kenny ,  Paul Yager

发明人： Caicai Wu ,  Bernhard Weigl ,  Margaret A. Kenny ,  Paul Yager

IPC分类号： G01N33543

CPC分类号： G01N33/5005 ,  G01N33/54313 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： This invention provides a method and apparatus for detecting the presence of analyte particles in a sample fluid also comprising larger particles, particularly blood. It exploits diffusion to provide simultaneous filtering of the larger particles and reaction of the analyte particles. A sample stream and a reagent stream join on the upstream end of a laminar flow reaction channel and flow in adjacent laminar streams. The reagents can be in solution or immobilized on a bead. The analyte particles diffuse from the sample stream into the reagent stream, leaving behind the larger particles in the residual sample stream. In the reagent stream the analyte particles react with reagent particles and form product particles, thereby creating a product stream. At the downstream end of the reaction channel, the residual sample stream and the product stream are divided. The product particles are then detected, preferably optically, in the product stream. Prior to detection, the product stream can undergo further filtering or separation, or can join a second reagent stream to form secondary product particles. This apparatus and method can be used to implement competitive immunoassays or sandwich immunoassays using enzymatically or fluorescently labeled immunoreagents. The apparatus and method can also be used to synthesize products, in which case two reagent streams join in the laminar flow reaction channel.

公开(公告)号：US06221677B1

公开(公告)日：2001-04-24

申请号：US09335930

申请日：1999-06-18

申请人： Caicai Wu ,  Bernhard Weigl ,  Margaret A. Kenny ,  Paul Yager

发明人： Caicai Wu ,  Bernhard Weigl ,  Margaret A. Kenny ,  Paul Yager

IPC分类号： G01N33543

CPC分类号： G01N33/5005 ,  G01N33/54313 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： This invention provides a method and apparatus for detecting the presence of analyte particles in a sample fluid also comprising larger particles, particularly blood. It exploits diffusion to provide simultaneous filtering of the larger particles and reaction of the analyte particles. A sample stream and a reagent stream join on the upstream end of a laminar flow reaction channel and flow in adjacent laminar streams. The reagents can be in solution or immobilized on a bead. The analyte particles diffuse from the sample stream into the reagent stream, leaving behind the larger particles in the residual sample stream. In the reagent stream the analyte particles react with reagent particles and form product particles, thereby creating a product stream. At the downstream end of the reaction channel, the residual sample stream and the product stream are divided. The product particles are then detected, preferably optically, in the product stream. Prior to detection, the product stream can undergo further filtering or separation, or can join a second reagent stream to form secondary product particles. This apparatus and method can be used to implement competitive immunoassays or sandwich immunoassays using enzymatically or fluorescently labeled immunoreagents. The apparatus and method can also be used to synthesize products, in which case two reagent streams join in the laminar flow reaction channel.

摘要翻译： 本发明提供了一种用于检测样品流体中分析物颗粒的存在的方法和装置，其还包括较大的颗粒，特别是血液。 它利用扩散来提供较大颗粒的同时过滤和分析物颗粒的反应。 样品流和试剂流在层流反应通道的上游端连接并在相邻层流中流动。 试剂可以溶于或固定在珠粒上。 分析物颗粒从样品流扩散到试剂流中，留下残余样品流中较大的颗粒。 在试剂流中，分析物颗粒与试剂颗粒反应并形成产物颗粒，从而产生产物流。 在反应通道的下游端，残留的样品流和产物流被分开。 然后在产品流中优选地以光学方式检测产物颗粒。 在检测之前，产物流可以经历进一步的过滤或分离，或可以连接第二试剂流以形成次级产物颗粒。 该装置和方法可用于实施使用酶或荧光标记免疫试剂的竞争性免疫测定或夹心免疫测定。 该装置和方法也可用于合成产物，在这种情况下，两个试剂流连接在层流反应通道中。

公开(公告)号：US6136272A

公开(公告)日：2000-10-24

申请号：US938584

申请日：1997-09-26

申请人： Bernhard Weigl ,  Diane M. Zebert ,  Margaret A. Kenny

发明人： Bernhard Weigl ,  Diane M. Zebert ,  Margaret A. Kenny

IPC分类号： B01F5/04 ,  B01F5/06 ,  B01F13/00 ,  B81B1/00 ,  G01N21/64

CPC分类号： B01F13/0059 ,  B01F13/0093 ,  B01F5/0471 ,  B01F5/0604 ,  B01F2005/0031 ,  Y10S366/01 ,  Y10S366/03 ,  Y10T436/255

摘要： A device and method for introducing a second laminar fluid layer to, or removing a second laminar fluid layer from, a first laminar fluid layer are provided. Each laminar fluid layer can contain two or more side by side laminar streams. The device includes a main flow channel, and at least one tributary channel in fluid connection with a bridge channel which is in fluid connection with main flow channel. The device can be formed in a single piece of material, which can be optically transparent. Optionally, the channels can be formed in a first plate, the first and optionally the second surfaces of which are sealed to a second and optionally a third plate. The second and third plates can be optically transparent to allow for optical detection and analysis. A first laminar fluid layer is introduced into the main flow channel. If a second laminar fluid layer is to be added to the first laminar fluid layer, then the former is introduced into the tributary channel, from whence it flows into the bridge channel and then into the main flow channel, where it flows below the first laminar fluid layer and diffusionally mixes with it. Preferably, the width of the main flow channel is relatively small, so that particles in an added second laminar fluid layer diffusionally mix into the first laminar fluid layer rapidly. If a second laminar fluid layer is to be removed from a first laminar fluid layer, then the latter is split into two portions: one portion continues flowing down the main flow channel and one portion flows into the bridge channel from whence it flows into the tributary channel.

摘要翻译： 提供了用于将第二层流体层引导到第一层流体层或从第一层流体层移除第二层流体层的装置和方法。 每个层流层可以包含两个或更多个并排层流。 该装置包括主流道，以及至少一个与与主流道流体连接的桥梁通道流体连接的支流通道。 该装置可以形成为单一材料，其可以是光学透明的。 可选地，通道可以形成在第一板中，第一和第二表面的第二表面被密封到第二和第三板。 第二和第三板可以是光学透明的，以允许光学检测和分析。 第一层流体层被引入到主流动通道中。 如果要将第二层流体层添加到第一层流体层中，则将前者引入支流通道中，从其流入桥通道然后进入主流动通道，在主流道中流动到第一层流 流体层并与其扩散混合。 优选地，主流动通道的宽度相对较小，使得添加的第二层流体层中的颗粒迅速地混合到第一层流体层中。 如果要从第一层流体层去除第二层流体层，则将其分成两部分：一部分继续沿着主流动通道向下流动，一部分流入桥梁通道，从而从其流入支流 渠道。

公开(公告)号：US06267962B1

公开(公告)日：2001-07-31

申请号：US08885323

申请日：1997-06-30

申请人： Ralph M. Hart ,  Herman L. Jones ,  Veronica Lee Egelkrout Jones ,  Sohail Malik ,  Margaret A. Kenny ,  Bernard Loev ,  James P. Harnisch

发明人： Ralph M. Hart ,  Herman L. Jones ,  Veronica Lee Egelkrout Jones ,  Sohail Malik ,  Margaret A. Kenny ,  Bernard Loev ,  James P. Harnisch

IPC分类号： A61K3578

CPC分类号： A61K33/30 ,  A61K33/06 ,  A61K33/14 ,  A61K33/24 ,  A61K33/32 ,  A61K33/34 ,  Y10S514/825 ,  Y10S514/858 ,  Y10S514/861 ,  Y10S514/863 ,  Y10S514/886 ,  A61K2300/00

摘要： Novel compositions containing at least one biologically active component derived from peat or similar composition, methods for their preparation and therapeutic uses for a variety of diseases, injuries, and conditions, including wound healing, pain, itch, inflammation, abnormal cell proliferation, or infections caused by fungal, bacterial, rickettsial or viral agents, psoriasis, allergic and other dermatitis, pruritis, eczema, actinic keratosis and similar conditions. In addition, the compositions can be used as diuretics, antiarrhythmics, and cardiac-stimulating agents, as well as for the treatment of mammalian diseases and disorders, including multiple drug resistance, cancers, asthma, rheumatoid arthritis, pain, wound healing, fungal disorders, and other inflammatory disorders. The compositions are derivable from peat or peat-related substances and may alternatively be synthetically produced.

摘要翻译： 含有至少一种衍生自泥炭或类似组合物的生物活性成分的新型组合物，其制备方法以及用于各种疾病，损伤和病症（包括伤口愈合，疼痛，瘙痒，炎症，异常细胞增殖或感染）的治疗用途 由真菌，细菌，立克次体或病毒药物引起的，牛皮癣，过敏性和其它皮炎，瘙痒症，湿疹，光化性角化病和类似的病症。 此外，组合物可用作利尿剂，抗心律失常药和心脏刺激剂，以及用于治疗哺乳动物疾病和病症，包括多重耐药性，癌症，哮喘，类风湿性关节炎，疼痛，伤口愈合，真菌性疾病 ，和其他炎性疾病。 所述组合物可衍生自泥炭或泥煤相关物质，并且可以合成地制备。