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    Simultaneous analyte determination and reference balancing in reference T-sensor devices
    1.
    发明授权
    Simultaneous analyte determination and reference balancing in reference T-sensor devices 失效
    参考T传感器设备中的同时分析物测定和参考平衡

    公开(公告)号：US5948684A

    公开(公告)日：1999-09-07

    申请号：US900926

    申请日：1997-07-25

    申请人： Bernhard H. Weigl ,  Mark R. Holl ,  Diane Zebert ,  Margaret Kenny ,  Caicai Wu

    发明人： Bernhard H. Weigl ,  Mark R. Holl ,  Diane Zebert ,  Margaret Kenny ,  Caicai Wu

    IPC分类号： G01N31/20 ,  B01F5/04 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N30/00 ,  G01N30/38 ,  G01N30/60 ,  G01N30/74 ,  G01N35/00 ,  G01N35/08 ,  G01N37/00

    CPC分类号： B01F13/0093 ,  B01F13/0059 ,  B01F5/0403 ,  B01J19/0093 ,  B01L3/502776 ,  G01N15/10 ,  G01N15/1484 ,  G01N30/0005 ,  B01L2200/0636 ,  B01L2200/148 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0487 ,  G01N15/14 ,  G01N15/1404 ,  G01N2035/00237 ,  G01N30/38 ,  G01N30/6095 ,  G01N30/74 ,  G01N35/085 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/2575

    摘要： A reference T-sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlet means in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or internal standard stream; c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area; and d) outlet means for conducting the streams out of the laminar flow channel preferably to form a single mixed stream. Also provided are methods of using the device to improve accuracy and increase convenience of measuring the concentration of analytes in a sample. Monitoring reference stream(s) in laminar flow with sample stream(s) allows for calibration or for monitoring or correcting for the effects of experimental conditions which might otherwise degrade accuracy. A reference stream which serves as both a control stream and an internal standard stream is convenient and economical. The device of this invention also needs less frequent calibration than previously-known fluid analysis devices because the devices and methods of this invention provide for continuous and simultaneous quality control and internal standardization.

    摘要翻译： 提供了一种参考T传感器系统，用于检测样品流中分析物颗粒的存在和/或测量浓度。 该系统包括：a）层流通道; b）与层流通道流体连接的三个或更多个入口装置，用于分别导入层流通道（1）中，指示物流可包括指示物质，其指示分析物颗粒的存在，（2）样品流 ，和（3）参考流，其可以是控制流和/或内部标准流; c）其中层流通道具有足够小的深度和/或宽度以允许流的层流，并且具有足以允许分析物的颗粒扩散到指示物流中以形成检测区域的长度; 以及d）用于将流引导出层流通道的出口装置优选地形成单一混合流。 还提供了使用该装置来提高测量样品中分析物浓度的准确性和增加方便性的方法。 用样品流监测层流中的参考流允许校准或监测或校正实际条件的影响，否则可能会降低准确度。 用作控制流和内部标准流的参考流是方便和经济的。 由于本发明的装置和方法提供连续和同时的质量控制和内部标准化，本发明的装置还需要比先前知道的流体分析装置更少的校准。

    Simultaneous particle separation and chemical reaction
    3.
    发明授权
    Simultaneous particle separation and chemical reaction 有权

    公开(公告)号：US06297061B1

    公开(公告)日：2001-10-02

    申请号：US09501732

    申请日：2000-02-10

    申请人： Caicai Wu ,  Bernhard Weigl ,  Margaret A. Kenny ,  Paul Yager

    发明人： Caicai Wu ,  Bernhard Weigl ,  Margaret A. Kenny ,  Paul Yager

    IPC分类号： G01N33543

    CPC分类号： G01N33/5005 ,  G01N33/54313 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

    摘要： This invention provides a method and apparatus for detecting the presence of analyte particles in a sample fluid also comprising larger particles, particularly blood. It exploits diffusion to provide simultaneous filtering of the larger particles and reaction of the analyte particles. A sample stream and a reagent stream join on the upstream end of a laminar flow reaction channel and flow in adjacent laminar streams. The reagents can be in solution or immobilized on a bead. The analyte particles diffuse from the sample stream into the reagent stream, leaving behind the larger particles in the residual sample stream. In the reagent stream the analyte particles react with reagent particles and form product particles, thereby creating a product stream. At the downstream end of the reaction channel, the residual sample stream and the product stream are divided. The product particles are then detected, preferably optically, in the product stream. Prior to detection, the product stream can undergo further filtering or separation, or can join a second reagent stream to form secondary product particles. This apparatus and method can be used to implement competitive immunoassays or sandwich immunoassays using enzymatically or fluorescently labeled immunoreagents. The apparatus and method can also be used to synthesize products, in which case two reagent streams join in the laminar flow reaction channel.

    Simultaneous analyte determination and reference balancing in reference T-sensor devices
    5.
    发明授权
    Simultaneous analyte determination and reference balancing in reference T-sensor devices 有权
    参考T传感器设备中的同时分析物测定和参考平衡

    公开(公告)号：US06171865B2

    公开(公告)日：2001-01-09

    申请号：US09366821

    申请日：1999-08-04

    申请人： Bernhard H. Weigl ,  Mark R. Holl ,  Diane Zebert ,  Margaret Kenny ,  Caicai Wu

    发明人： Bernhard H. Weigl ,  Mark R. Holl ,  Diane Zebert ,  Margaret Kenny ,  Caicai Wu

    IPC分类号： G01N3508

    CPC分类号： B01F13/0093 ,  B01F5/0403 ,  B01F13/0059 ,  B01J19/0093 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/148 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0487 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1484 ,  G01N30/0005 ,  G01N30/38 ,  G01N30/6095 ,  G01N30/74 ,  G01N35/085 ,  G01N2035/00237 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/2575

    摘要： A reference sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlet means in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or internal standard stream; and, c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of said streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area. Branching channels may be provided as outlet means for conducting the streams out of the laminar flow channel. Also provided are methods of using the device to improve accuracy and increase convenience of measuring the concentration of analytes in a sample. Monitoring reference stream(s) in laminar flow with sample stream(s) allows for calibration or for monitoring or correcting for the effects of experimental conditions which might otherwise degrade accuracy. A reference stream which serves as both a control stream and an internal standard stream is convenient and economical. The device of this invention also needs less frequent calibration than previously-known fluid analysis devices because the devices and methods of this invention provide for continuous and simultaneous quality control and internal standardization.

    摘要翻译： 提供了一种用于检测样品流中分析物颗粒的存在和/或测量浓度的参考传感器系统。 该系统包括：a）层流通道; b）与层流通道流体连接的三个或更多个入口装置，用于分别导入层流通道（1）中，指示物流可包括指示物质，其指示分析物颗粒的存在，（2）样品流 ，和（3）参考流，其可以是控制流和/或内部标准流; 和c）其中所述层流通道的深度和/或宽度足够小以允许所述物流的层流并且足够长度以允许所述分析物的颗粒扩散到所述指示物流中以形成检测区域。 可以提供分支通道作为用于将流引导出层流通道的出口装置。 还提供了使用该装置来提高测量样品中分析物浓度的准确性和增加方便性的方法。 用样品流监测层流中的参考流允许校准或监测或校正实际条件的影响，否则可能会降低准确度。 用作控制流和内部标准流的参考流是方便和经济的。 由于本发明的装置和方法提供连续和同时的质量控制和内部标准化，本发明的装置还需要比先前知道的流体分析装置更少的校准。

    Simultaneous particle separation and chemical reaction
    6.
    发明授权
    Simultaneous particle separation and chemical reaction 有权
    同时进行颗粒分离和化学反应

    公开(公告)号：US06221677B1

    公开(公告)日：2001-04-24

    申请号：US09335930

    申请日：1999-06-18

    申请人： Caicai Wu ,  Bernhard Weigl ,  Margaret A. Kenny ,  Paul Yager

    发明人： Caicai Wu ,  Bernhard Weigl ,  Margaret A. Kenny ,  Paul Yager

    IPC分类号： G01N33543

    CPC分类号： G01N33/5005 ,  G01N33/54313 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

    摘要： This invention provides a method and apparatus for detecting the presence of analyte particles in a sample fluid also comprising larger particles, particularly blood. It exploits diffusion to provide simultaneous filtering of the larger particles and reaction of the analyte particles. A sample stream and a reagent stream join on the upstream end of a laminar flow reaction channel and flow in adjacent laminar streams. The reagents can be in solution or immobilized on a bead. The analyte particles diffuse from the sample stream into the reagent stream, leaving behind the larger particles in the residual sample stream. In the reagent stream the analyte particles react with reagent particles and form product particles, thereby creating a product stream. At the downstream end of the reaction channel, the residual sample stream and the product stream are divided. The product particles are then detected, preferably optically, in the product stream. Prior to detection, the product stream can undergo further filtering or separation, or can join a second reagent stream to form secondary product particles. This apparatus and method can be used to implement competitive immunoassays or sandwich immunoassays using enzymatically or fluorescently labeled immunoreagents. The apparatus and method can also be used to synthesize products, in which case two reagent streams join in the laminar flow reaction channel.

    摘要翻译： 本发明提供了一种用于检测样品流体中分析物颗粒的存在的方法和装置，其还包括较大的颗粒，特别是血液。 它利用扩散来提供较大颗粒的同时过滤和分析物颗粒的反应。 样品流和试剂流在层流反应通道的上游端连接并在相邻层流中流动。 试剂可以溶于或固定在珠粒上。 分析物颗粒从样品流扩散到试剂流中，留下残余样品流中较大的颗粒。 在试剂流中，分析物颗粒与试剂颗粒反应并形成产物颗粒，从而产生产物流。 在反应通道的下游端，残留的样品流和产物流被分开。 然后在产品流中优选地以光学方式检测产物颗粒。 在检测之前，产物流可以经历进一步的过滤或分离，或可以连接第二试剂流以形成次级产物颗粒。 该装置和方法可用于实施使用酶或荧光标记免疫试剂的竞争性免疫测定或夹心免疫测定。 该装置和方法也可用于合成产物，在这种情况下，两个试剂流连接在层流反应通道中。