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公开(公告)号：US20180193838A1
公开(公告)日：2018-07-12
申请号：US15861225
申请日：2018-01-03
IPC分类号： B01L3/00 , C12Q1/44 , B01F3/08 , B01F5/06 , B01F13/00 , C12P19/34 , B01J19/00 , G01N15/14 , G01N35/08 , B01L7/00 , B82Y30/00
CPC分类号： B01L3/502784 , B01F3/0861 , B01F5/0403 , B01F5/0646 , B01F5/0647 , B01F13/0071 , B01F2215/0037 , B01J19/0046 , B01J19/0093 , B01J2219/00286 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00725 , B01J2219/00736 , B01J2219/0074 , B01J2219/00756 , B01J2219/00783 , B01J2219/00837 , B01J2219/0086 , B01J2219/00867 , B01J2219/00869 , B01J2219/00889 , B01J2219/00891 , B01J2219/00975 , B01J2219/00977 , B01L3/502746 , B01L3/502761 , B01L7/52 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2200/10 , B01L2300/0867 , B01L2300/0883 , B01L2400/0424 , B01L2400/0448 , B01L2400/0454 , B01L2400/0487 , B82Y30/00 , C12P19/34 , C12Q1/44 , G01N15/1404 , G01N15/1484 , G01N35/08 , G01N35/085 , G01N2015/1409 , Y02A90/26 , Y10T436/118339 , Y10T436/12 , Y10T436/2575
摘要： The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.
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公开(公告)号：US09968933B2
公开(公告)日：2018-05-15
申请号：US15673955
申请日：2017-08-10
CPC分类号： B01L3/502784 , B01F3/0861 , B01F5/0403 , B01F5/0646 , B01F5/0647 , B01F13/0071 , B01F2215/0037 , B01J19/0046 , B01J19/0093 , B01J2219/00286 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00725 , B01J2219/00736 , B01J2219/0074 , B01J2219/00756 , B01J2219/00783 , B01J2219/00837 , B01J2219/0086 , B01J2219/00867 , B01J2219/00869 , B01J2219/00889 , B01J2219/00891 , B01J2219/00975 , B01J2219/00977 , B01L3/502746 , B01L3/502761 , B01L7/52 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2200/10 , B01L2300/0867 , B01L2300/0883 , B01L2400/0424 , B01L2400/0448 , B01L2400/0454 , B01L2400/0487 , B82Y30/00 , C12P19/34 , C12Q1/44 , G01N15/1404 , G01N15/1484 , G01N35/08 , G01N35/085 , G01N2015/1409 , Y02A90/26 , Y10T436/118339 , Y10T436/12 , Y10T436/2575
摘要： The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.
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公开(公告)号：US20180117585A1
公开(公告)日：2018-05-03
申请号：US15829371
申请日：2017-12-01
CPC分类号： B01L3/5027 , B01F5/0256 , B01F5/0646 , B01F5/0655 , B01F5/0682 , B01F5/0689 , B01F13/0069 , B01F13/0071 , B01F13/0074 , B01F13/0076 , B01J19/0093 , B01J2219/00783 , B01J2219/00862 , B01L3/0241 , B01L3/502761 , B01L3/502776 , B01L3/502784 , B01L3/502792 , B01L3/5088 , B01L2200/0636 , B01L2200/0652 , B01L2200/0673 , B01L2300/0864 , B01L2300/0867 , B01L2300/161 , B01L2400/0415 , B01L2400/0439 , B01L2400/0487 , C12Q2563/159 , C12Q2565/629 , G01N15/10 , G01N15/14 , G01N15/1459 , G01N15/1484 , G01N2015/1006 , G01N2015/1081 , G01N2015/149 , Y10S436/807 , Y10T436/118339 , Y10T436/2525 , Y10T436/2575
摘要： Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction. In a related aspect of the invention, systems and methods for allowing fluid mixing within droplets to occur are also provided. In still another aspect, the invention relates to systems and methods for sorting droplets, e.g., by causing droplets to move to certain regions within a fluidic system. Examples include using electrical interactions (e.g., charges, dipoles, etc.) or mechanical systems (e.g., fluid displacement) to sort the droplets. In some cases, the fluidic droplets can be sorted at relatively high rates, e.g., at about 10 droplets per second or more. Another aspect of the invention provides the ability to determine droplets, or a component thereof, for example, using fluorescence and/or other optical techniques (e.g., microscopy), or electric sensing techniques such as dielectric sensing.
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公开(公告)号：US20170361318A1
公开(公告)日：2017-12-21
申请号：US15695184
申请日：2017-09-05
CPC分类号： B01L3/5027 , B01F5/0256 , B01F5/0646 , B01F5/0655 , B01F5/0682 , B01F5/0689 , B01F13/0069 , B01F13/0071 , B01F13/0074 , B01F13/0076 , B01J19/0093 , B01J2219/00783 , B01J2219/00862 , B01L3/0241 , B01L3/502761 , B01L3/502776 , B01L3/502784 , B01L3/502792 , B01L3/5088 , B01L2200/0636 , B01L2200/0652 , B01L2200/0673 , B01L2300/0864 , B01L2300/0867 , B01L2300/161 , B01L2400/0415 , B01L2400/0439 , B01L2400/0487 , C12Q2563/159 , C12Q2565/629 , G01N15/10 , G01N15/14 , G01N15/1459 , G01N15/1484 , G01N2015/1006 , G01N2015/1081 , G01N2015/149 , Y10S436/807 , Y10T436/118339 , Y10T436/2525 , Y10T436/2575
摘要： Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction. In a related aspect of the invention, systems and methods for allowing fluid mixing within droplets to occur are also provided. In still another aspect, the invention relates to systems and methods for sorting droplets, e.g., by causing droplets to move to certain regions within a fluidic system. Examples include using electrical interactions (e.g., charges, dipoles, etc.) or mechanical systems (e.g., fluid displacement) to sort the droplets. In some cases, the fluidic droplets can be sorted at relatively high rates, e.g., at about 10 droplets per second or more. Another aspect of the invention provides the ability to determine droplets, or a component thereof, for example, using fluorescence and/or other optical techniques (e.g., microscopy), or electric sensing techniques such as dielectric sensing.
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公开(公告)号：US09839893B2
公开(公告)日：2017-12-12
申请号：US14836432
申请日：2015-08-26
IPC分类号： B01L3/00 , B01J19/00 , B01F5/06 , B01F13/00 , G01N35/08 , C12P19/34 , C12Q1/44 , B82Y30/00 , G01N15/14
CPC分类号： B01L3/502784 , B01F3/0861 , B01F5/0403 , B01F5/0646 , B01F5/0647 , B01F13/0071 , B01F2215/0037 , B01J19/0046 , B01J19/0093 , B01J2219/00286 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00725 , B01J2219/00736 , B01J2219/0074 , B01J2219/00756 , B01J2219/00783 , B01J2219/00837 , B01J2219/0086 , B01J2219/00867 , B01J2219/00869 , B01J2219/00889 , B01J2219/00891 , B01J2219/00975 , B01J2219/00977 , B01L3/502746 , B01L3/502761 , B01L7/52 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2200/10 , B01L2300/0867 , B01L2300/0883 , B01L2400/0424 , B01L2400/0448 , B01L2400/0454 , B01L2400/0487 , B82Y30/00 , C12P19/34 , C12Q1/44 , G01N15/1404 , G01N15/1484 , G01N35/08 , G01N35/085 , G01N2015/1409 , Y02A90/26 , Y10T436/118339 , Y10T436/12 , Y10T436/2575
摘要： The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.
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公开(公告)号：US20160216231A1
公开(公告)日：2016-07-28
申请号：US15087020
申请日：2016-03-31
IPC分类号： G01N27/447 , G01N33/543 , G01N15/10 , C12Q1/68
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
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公开(公告)号：US20160209303A1
公开(公告)日：2016-07-21
申请号：US15082424
申请日：2016-03-28
发明人： Benjamin Miller , Brian Hutchison , Andrew Wilson , Jonathan William Larson , Qun Zhong , Yevgeny Yurkovetsky , Darren Link , Mark Weary
CPC分类号： G01N1/38 , B01L3/0241 , B01L3/502715 , B01L3/502784 , B01L2200/061 , B01L2200/0673 , B01L2200/143 , B01L2300/0877 , F16K99/0001 , F16K2099/0084 , F17D3/01 , G01N27/44791 , G01N2021/0353 , Y10T137/2185 , Y10T137/7761 , Y10T137/8158 , Y10T137/8593 , Y10T436/118339
摘要： The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.
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公开(公告)号：US20150330943A1
公开(公告)日：2015-11-19
申请号：US14805532
申请日：2015-07-22
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a method of providing a droplet comprising a single cell is provided, wherein the method includes providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium, dispensing from the droplet to provide a dispensed droplet, determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell, and sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations.
摘要翻译： 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了一种提供包括单个电池的液滴的方法，其中所述方法包括提供液滴，其包含由油介质包围的电润湿液滴致动器上的悬浮液，从液滴分配以提供分配的液滴 确定电润湿液滴致动器上的分配液滴是否包括单个电池，并且基于使用电润湿介导的液滴操作的确定步骤的结果，将分配的液滴分配在电润湿液滴致动器上。
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公开(公告)号：US09157922B2
公开(公告)日：2015-10-13
申请号：US14330548
申请日：2014-07-14
发明人： Yuji Saito , Fumio Takagi
CPC分类号： G01N35/1002 , B01L3/0241 , B01L7/52 , B01L2300/0838 , B01L2400/0457 , B01L2400/049 , G01N1/10 , G01N1/14 , G01N1/38 , G01N35/1016 , Y10T436/118339 , Y10T436/119163 , Y10T436/25 , Y10T436/2575
摘要： A dispensing method which dispenses a first liquid contained in a first vessel which stores the first liquid and a second liquid to introduce the first liquid into a second vessel by using a tube.
摘要翻译： 一种分配方法，其分配容纳在存储第一液体的第一容器中的第一液体和第二液体，以通过使用管将第一液体引入第二容器。
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10.发明申请APPARATUS AND METHODS FOR INTEGRATED SAMPLE PREPARATION, REACTION AND DETECTION 审中-公开集成样品制备，反应和检测的装置和方法公开(公告)号：US20150258548A1
公开(公告)日：2015-09-17
申请号：US14723586
申请日：2015-05-28
申请人： LUMINEX CORPORATION
CPC分类号： B01L7/52 , B01F5/12 , B01F11/0071 , B01F15/0203 , B01F15/0237 , B01F2015/0221 , B01F2215/0037 , B01L3/502 , B01L3/502715 , B01L3/50851 , B01L3/523 , B01L3/527 , B01L7/00 , B01L2200/025 , B01L2200/026 , B01L2200/028 , B01L2200/0647 , B01L2200/10 , B01L2200/16 , B01L2300/042 , B01L2300/044 , B01L2300/0627 , B01L2300/0654 , B01L2300/0672 , B01L2300/0829 , B01L2300/0867 , B01L2300/087 , B01L2300/1805 , B01L2400/0406 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0622 , B01L2400/0633 , B01L2400/0644 , B01L2400/0683 , C12Q1/6806 , C12Q1/686 , G01N1/38 , G01N35/1079 , G01N2035/0436 , Y10T436/118339 , Y10T436/25375 , Y10T436/255
摘要： Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.
摘要翻译： 还提供了用于分离生物样品和样品上的下游生物测定的柱，以及使用这种盒的方法。 在一个实施方案中，从生物样品中分离核酸样品，并且例如通过聚合酶链式反应扩增核酸样品。 本文提供的盒还可用于分离非核酸样品，例如蛋白质，并对蛋白质进行下游反应，例如结合测定。 还提供了用于进行下游生物测定和检测测定结果的仪器。
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