公开(公告)号：US20020005354A1

公开(公告)日：2002-01-17

申请号：US09928590

申请日：2001-08-13

Applicant: California Institute of Technology

Inventor： Charles F. Spence ,  Anne Y. Fu ,  Stephen R. Quake ,  Frances H. Arnold

IPC: C12M001/36 ,  C12M001/00 ,  C12M001/34 ,  C12M001/42 ,  G01N027/26 ,  G01N027/447

CPC classification number: G01N27/26 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0454 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N15/14 ,  G01N2015/149

Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.

Abstract translation: 本发明提供了一种用于基于所需特征分选细胞的微制造装置，例如，报道分子标记的细胞可以通过细胞上报告物的存在或水平进行分选。 该装置包括具有基板的芯片，微基板将至少一个分析单元微加工。 每个分析单元包括主通道，具有通常在一端的样品入口通道和沿其长度的一部分的检测区域。 相邻和下游的检测区域，主通道具有通向至少两个分支通道的识别区域或分支点。 分析单元还可以根据需要进一步包括额外的入口通道，检测点，分支点和分支通道。 包含细胞的流通过检测区域，使得平均一个细胞在给定时间占据检测区域。 基于可检测信号（例如光信号）的存在或量，可以将细胞分类到适当的分支通道中，具有或不具有刺激，例如暴露于光以促进荧光。