公开(公告)号：US20020127736A1

公开(公告)日：2002-09-12

申请号：US09970122

申请日：2001-10-02

Applicant: California Institute of Technology

Inventor： Hou-Pu Chou ,  Anne Y. Fu ,  Stephen R. Quake

IPC: B01L003/00

CPC classification number: F04B43/00 ,  A61M2206/22 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/0418 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/084 ,  F04B11/00 ,  F04B19/006 ,  F04B43/043 ,  G01N30/6095 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2030/205 ,  G01N2030/322 ,  Y10T436/2575

Abstract: A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality.

Abstract translation: 微流体装置包括泵，阀和流体振荡阻尼器。 在用于排序的装置中，实体通过泵通过检测区域的流动通道流到结。 基于在检测区域中确定的实体的身份，致动位于连接处的流动通道的相对分支上的废物或收集阀，从而将该实体路由到废物池或收集池。 阻尼器结构可以位于泵和接头之间。 阻尼器由于泵的操作而减小了流动通道中的振荡压力的振幅，从而减少了分离过程中实体的速度振荡。 微流体装置可以形成为弹性体材料块，弹性体材料的薄膜可偏转到流动通道中以提供泵或阀的功能。

公开(公告)号：US20020005354A1

公开(公告)日：2002-01-17

申请号：US09928590

申请日：2001-08-13

Applicant: California Institute of Technology

Inventor： Charles F. Spence ,  Anne Y. Fu ,  Stephen R. Quake ,  Frances H. Arnold

IPC: C12M001/36 ,  C12M001/00 ,  C12M001/34 ,  C12M001/42 ,  G01N027/26 ,  G01N027/447

CPC classification number: G01N27/26 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0454 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N15/14 ,  G01N2015/149

Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.

Abstract translation: 本发明提供了一种用于基于所需特征分选细胞的微制造装置，例如，报道分子标记的细胞可以通过细胞上报告物的存在或水平进行分选。 该装置包括具有基板的芯片，微基板将至少一个分析单元微加工。 每个分析单元包括主通道，具有通常在一端的样品入口通道和沿其长度的一部分的检测区域。 相邻和下游的检测区域，主通道具有通向至少两个分支通道的识别区域或分支点。 分析单元还可以根据需要进一步包括额外的入口通道，检测点，分支点和分支通道。 包含细胞的流通过检测区域，使得平均一个细胞在给定时间占据检测区域。 基于可检测信号（例如光信号）的存在或量，可以将细胞分类到适当的分支通道中，具有或不具有刺激，例如暴露于光以促进荧光。