公开(公告)号：US10345572B2

公开(公告)日：2019-07-09

申请号：US15422241

申请日：2017-02-01

Applicant: California Institute of Technology

Inventor： Eugene Serabyn ,  Christian A. Lindensmith ,  James K. Wallace ,  Kurt M. Liewer ,  Jay L. Nadeau

IPC: G02B23/24 ,  G02B3/00 ,  F21V8/00 ,  G03H1/04 ,  G02B21/36 ,  G02B23/26 ,  G03H1/00

Abstract: In situ investigation of microbial life in extreme environments can be carried out with microscopes capable of imaging 3-dimensional volumes and tracking particle motion. A lensless digital holographic microscope is disclosed that provides roughly 1.5 micron resolution in a compact, robust package suitable for remote deployment. High resolution is achieved by generating high numerical-aperture input beams with radial gradient-index rod lenses. The ability to detect and track prokaryotes was explored using bacterial strains of two different sizes. In the larger strain, a variety of motions were seen, while the smaller strain was used to demonstrate a detection capability down to micron scales.

公开(公告)号：US20170219999A1

公开(公告)日：2017-08-03

申请号：US15422241

申请日：2017-02-01

Applicant: California Institute of Technology

Inventor： Eugene Serabyn ,  Christian A. Lindensmith ,  James K. Wallace ,  Kurt M. Liewer ,  Jay L. Nadeau

IPC: G03H1/00 ,  G02B23/24 ,  G03H1/04 ,  G02B21/36 ,  G02B3/00 ,  F21V8/00

CPC classification number: G02B23/2484 ,  G02B3/0087 ,  G02B6/0005 ,  G02B6/0006 ,  G02B6/0008 ,  G02B21/365 ,  G02B23/2469 ,  G02B23/26 ,  G03H1/0443 ,  G03H1/0465 ,  G03H2001/005 ,  G03H2001/0436 ,  G03H2001/0445 ,  G03H2001/0469 ,  G03H2001/0471 ,  G03H2222/52 ,  G03H2223/17

Abstract: In situ investigation of microbial life in extreme environments can be carried out with microscopes capable of imaging 3-dimensional volumes and tracking particle motion. A lensless digital holographic microscope is disclosed that provides roughly 1.5 micron resolution in a compact, robust package suitable for remote deployment. High resolution is achieved by generating high numerical-aperture input beams with radial gradient-index rod lenses. The ability to detect and track prokaryotes was explored using bacterial strains of two different sizes. In the larger strain, a variety of motions were seen, while the smaller strain was used to demonstrate a detection capability down to micron scales.