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公开(公告)号：US10048478B2
公开(公告)日：2018-08-14
申请号：US15036582
申请日：2014-11-13
发明人： Artur Degen , Michael Gölles , Jörg Siebenmorgen , Helmut Lippert , Thomas Kalkbrenner , Ingo Kleppe , Matthias Wald , Lars-Christian Wittig , Wolfgang Singer , Ralf Wolleschensky
IPC分类号： G02B21/06 , G02B21/00 , G02B27/00 , G02B21/02 , G02B21/36 , G02B3/04 , G02B21/24 , G02B21/33 , G02B21/34
摘要： An optical transmission system configured to image a selected region of a sample arranged in a first medium in an object plane on or in a sample carrier, which includes plane-parallel plate, from the object plane into an intermediate image plane in a second medium. The plane-parallel plate is located between the optical transmission system and the sample during the imaging. The object plane and the intermediate image plane form an angle between 0° and 90° with an optical axis of the transmission system. The optical transmission system is positioned relative to region of the sample such that the sample is located within the focal length of the lens of the optical transmission system closest to the sample. The intermediate image plane and the object plane are located on the same side of the optical transmission system, and the intermediate image is a virtual image.
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公开(公告)号：US11163147B2
公开(公告)日：2021-11-02
申请号：US16313645
申请日：2017-06-29
发明人： Jörg Siebenmorgen , Helmut Lippert , Thomas Kalkbrenner , Ingo Kleppe , Ralf Wolleschensky , Artur Degen , Matthias Wald , Lars-Christian Wittig , Michael Gölles , Wolfgang Singer
摘要： An arrangement for microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen on a specimen carrier. An optical axis of the illumination objective lies in a plane which includes an illumination angle that differs from zero with the normal of a specimen plane and the illumination is implemented in the plane. A detection optical unit with a detection objective is located in a detection beam path. The optical axis of the detection objective includes a detection angle that differs from zero with the normal of the specimen plane. The illumination objective and/or the detection objective has an illumination correction element. A meniscus lens is located between the specimen carrier and the illumination and detection objectives being arranged both in the illumination beam path and in the detection beam path.
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公开(公告)号：US10073256B2
公开(公告)日：2018-09-11
申请号：US14894029
申请日：2014-05-27
CPC分类号： G02B21/04 , G02B13/22 , G02B17/0808 , G02B17/084 , G02B17/0856 , G02B17/0896 , G02B21/0076 , G02B21/06 , G02B21/33 , G02B21/367
摘要： An apparatus for imaging a sample arranged in a first medium in an object plane. The apparatus includes an optical transmission system which images the sample in the object plane in an intermediate image in an intermediate image plane. The object plane and the intermediate image plane form an angle not equal to 90° with an optical axis of the transmission system. The apparatus further comprises an optical imaging system having an objective. The object plane may be imaged on a detector without distortion. The optical transmission system is symmetrical with respect to a pupil plane, the object plane, and the intermediate image plane to satisfy the Scheimpflug condition. The intermediate image lies in a second medium having a refractive index virtually identical to that of the first medium. A lens group of a subsystem arranged closest to the sample or intermediate image comprises at least one catadioptric assembly.
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公开(公告)号：US10345567B2
公开(公告)日：2019-07-09
申请号：US13968220
申请日：2013-08-15
发明人： Artur Degen , Daniel Schwedt , Michael Gölles
摘要： A laser scanning microscope having a laser source for fluorescence excitation; a scanning mirror arrangement for scanning a specimen and scanning optics for generating a diffraction-limited reference image plane as a first intermediate image plane; an optical system for demagnified imaging of the reference plane in a second intermediate image plane; an axially slideable mirror in the second intermediate image plane; a beam splitter arrangement between the reference image plane and the optical system; and a tube lens and a first microscope objective for imaging of the reference image plane into a specimen. The imaging of the image is effected with a magnification of M≠n/n′ and/or a magnification of the second intermediate image plane into the specimen according to the equation M = y ′ y = n n ′ ξ , with |ξ|≠1, and/or the focal plane of the laser deviates from the axial position of the axially movable mirror across its scanning area in the second intermediate image plane.
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公开(公告)号：US20140055851A1
公开(公告)日：2014-02-27
申请号：US13968220
申请日：2013-08-15
发明人： Artur Degen , Daniel Schwedt , Michael Gölles
IPC分类号： G02B21/00
CPC分类号： G02B21/0072 , G02B21/0056 , G02B21/0076 , G02B21/02
摘要： A laser scanning microscope having a laser light source for fluorescence excitation, in particular a short pulse light source for multiphoton excitation; a scanning mirror array for scanning illumination of a specimen and a scanning optics for the generation of a diffraction-limited reference image plane as a first intermediate image plane; an optical system, preferably with a high numerical aperture, for the preferably demagnified imaging of the reference plane in a second intermediate image; an axially slideable mirror in the second intermediate image plane; a beam splitter array between the reference image plane and said optical system; and a tube lens and a first microscope objective for aberration-free imaging of the reference image plane in a specimen. Imaging of the image M of the second intermediate image plane in the specimen is effected with a magnification of M≠n/n′ and/or a magnification M of the second intermediate image plane in the specimen according to the equation M=y′/y=n/n′ξ, with |ξ|≠1 deviating by ξ≠1 from the quotient of the refractive indexes n, n′ of the immersion media in the object space on the specimen and in the image space on the mirror, and/or the focal plane of the excitation laser generated by the optical system deviates from the axial position of the mirror across basically the entire scanning area of the mirror in the intermediate image plane, such that the maximum energy density in the laser spot on the mirror surface area remains constantly below the destruction threshold of the reflecting layer and/or the image of the mirror surface area in the object space is located at least four depths of field from the focal plane of the laser in the object space and/or the second intermediate image exhibits strong aberrations.
摘要翻译： 一种具有用于荧光激发的激光光源的激光扫描显微镜，特别是用于多光子激发的短脉冲光源; 用于扫描样本的扫描反射镜阵列和用于产生衍射极限参考图像平面的扫描光学器件作为第一中间像面; 光学系统，优选地具有高数值孔径，用于在第二中间图像中优选地对参考平面成像; 在第二中间像平面中的可轴向滑动的反射镜; 在参考图像平面和所述光学系统之间的分束器阵列; 以及用于在样本中的参考像平面的无像差成像的管透镜和第一显微镜物镜。 根据等式M = y'，以样本中的第二中间像面的成像M以样本中的第二中间像平面的放大倍数M
1与样品上的物体空间中的浸没介质的折射率n，n'的折射率乘以xi <1，并且在图像空间中 由光学系统产生的激光激光的反射镜和/或焦平面偏离反射镜的轴向位置，基本上在中间像平面中的反射镜的整个扫描区域上，使得激光器中的最大能量密度 镜面区域上的斑点保持恒定低于反射层的破坏阈值，和/或物体空间中的镜面区域的图像位于物体空间中激光焦点的至少四个深度的场， /或第二中间图像显示s 畸形畸形 -
公开(公告)号：US09804378B2
公开(公告)日：2017-10-31
申请号：US15036460
申请日：2014-11-13
发明人： Wolfgang Singer , David Shafer , Artur Degen , Jörg Siebenmorgen
CPC分类号： G02B21/08 , G02B13/18 , G02B21/0032 , G02B21/02 , G02B21/18 , G02B27/0025
摘要： An arrangement for light sheet microscopy including illumination optics with an illumination objective for illuminating a sample, located in a medium on a sample carrier aligned with respect to a plane reference surface, with a light sheet. The arrangement also includes detection optics with a detection objective. The arrangement further includes a separating layer system with at least one layer separating the medium from the illumination and detection objectives. The separating layer system contacts the medium by a base surface aligned parallel to the reference surface. A correction lens system, with at least one correction lens serving to reduce those aberrations which occur as a result of the oblique passage of illumination light and/or of light to be detected through interfaces of the separating layer system, is arranged between illumination objective and separating layer system and/or between detection objective and separating layer system.
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