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公开(公告)号：US11308688B2
公开(公告)日：2022-04-19
申请号：US16573869
申请日：2019-09-17
摘要： A localization microscope including an imaging device emitting sample light from a focal plane into an image plane, including an optical-manipulation device for depth-dependent influencing of a point-spread function of the imaging and influencing the point-spread function of the imaging such that a point emitter is imaged in the image plane into an image that is rotationally asymmetrically distorted. A form of the distortion depends on the location of the point emitter with respect to the focal plane and a wavelength of the sample light. The optical manipulation device includes first and second anisotropy elements that anisotropically influence the point spread function to produce rotational asymmetry of the point emitter image. The elements are arranged one behind the other in the imaging direction, with anisotropy axes at an angle to one another. Both elements have differing neutral wavelength at which they do not anisotropically influence the point spread.
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公开(公告)号：US11163147B2
公开(公告)日：2021-11-02
申请号：US16313645
申请日：2017-06-29
发明人： Jörg Siebenmorgen , Helmut Lippert , Thomas Kalkbrenner , Ingo Kleppe , Ralf Wolleschensky , Artur Degen , Matthias Wald , Lars-Christian Wittig , Michael Gölles , Wolfgang Singer
摘要： An arrangement for microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen on a specimen carrier. An optical axis of the illumination objective lies in a plane which includes an illumination angle that differs from zero with the normal of a specimen plane and the illumination is implemented in the plane. A detection optical unit with a detection objective is located in a detection beam path. The optical axis of the detection objective includes a detection angle that differs from zero with the normal of the specimen plane. The illumination objective and/or the detection objective has an illumination correction element. A meniscus lens is located between the specimen carrier and the illumination and detection objectives being arranged both in the illumination beam path and in the detection beam path.
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公开(公告)号：US11119301B2
公开(公告)日：2021-09-14
申请号：US16144839
申请日：2018-09-27
发明人： Thomas Ohrt , Thomas Kalkbrenner
摘要： The invention relates to an immersion matrix (5), designed for adjusting optical properties at interfaces of optical arrangements, having a porosity with pores of at least one pore size selected from a range from >20 nm to 200 μm and/or nanopores in the material of the immersion matrix (5), wherein the nanopores have at least one average pore size selected from a range of 0.5 nm to 20 nm, and an elasticity modulus E selected from a range of 0.1-100 MPa. The invention furthermore relates to the use of the immersion matrix (5), an arrangement with the immersion matrix and an immersion device.
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4.发明授权公开(公告)号：US10477124B2
公开(公告)日：2019-11-12
申请号：US15514453
申请日：2015-08-31
摘要： A device and a method for imaging a sample arranged in an object plane. The device includes an optical relay system that images an area of the sample from the object plane into an intermediate image plane. The device may also include an optical imaging system with an objective having an optical axis that lies perpendicularly on the intermediate image plan, and which is focused on the intermediate image plane, with the result that the object plane can be imaged undistorted onto a detector. The device also can include an illumination apparatus for illuminating the sample with a light sheet, wherein the light sheet lies essentially in the object plane and defines an illumination direction, and wherein the normal of the object plane defines a detection direction.
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公开(公告)号：US10088661B2
公开(公告)日：2018-10-02
申请号：US15431894
申请日：2017-02-14
摘要： An adjusting mechanism of a sample holder is provided. The adjusting mechanism includes a base with drives arranged thereon, and a carrier that is adjustable by means of the drives and is designed to receive the sample holder. A coupling element for each drive, which coupling element is designed to connect the base and the carrier. Each coupling element has at least one linear degree of freedom and also a rotary degree of freedom. The carrier is linearly movable, by means of the coupling elements, along a respective movement axis directed from the coupling element to the carrier. Also provided is a microscope that includes such an adjusting mechanism, along with a method for adjusting the orientation of a sample holder.
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公开(公告)号：US10078206B2
公开(公告)日：2018-09-18
申请号：US15087177
申请日：2016-03-31
IPC分类号： G02B21/36 , G02B21/00 , G01N21/64 , G02B21/18 , G02B17/02 , G02B21/16 , G02B27/10 , G02B27/14 , G02B27/58 , G02B7/38 , G02B21/24
CPC分类号： G02B21/361 , G01N21/6458 , G02B7/38 , G02B17/023 , G02B21/0032 , G02B21/0072 , G02B21/0076 , G02B21/0088 , G02B21/16 , G02B21/18 , G02B21/245 , G02B21/367 , G02B27/106 , G02B27/1066 , G02B27/141 , G02B27/144 , G02B27/145 , G02B27/58
摘要： The invention relates to a microscope having an illumination beam path with wide field illumination of a sample and a first detection beam path having a spatially resolved surface receiver, which is reached by a first part of the detection light coming from the sample via the first detection beam path, or an image divider assembly for a microscope. In order to lengthen the optical path length, at least a second part of the detection light coming from the sample is masked out of the detection beam path and, via deflection means belonging to the detection beam path, is led into a second detection beam path and, preferably via further deflection means, is deflected back in the direction of the detection in such a way that detection light is applied to at least two partial regions beside one another on the surface receiver. At least the second part of the detection light runs in an optical element having an optical density that is increased as compared with the first detection beam path, in order to lengthen the optical path length, and the optical element is designed to be displaceable at an angle, preferably perpendicular, to the optical axis of the first detection beam path in order to adjust the optical path length, and has flat surfaces, at least on the light entry and light exit side thereof; a prism is provided, preferably a glass prism, preferably at least in the second detection beam path after a first beam deflection, for deflection in a direction parallel to the first detection beam path, in order to increase the path length and for reverse deflection.
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公开(公告)号：US09885860B2
公开(公告)日：2018-02-06
申请号：US14373740
申请日：2012-12-13
CPC分类号： G02B21/16 , G01N21/6458 , G01N2201/06113 , G01N2201/12 , G02B21/361 , G02B21/367 , G02B27/58
摘要： A method for high-resolution 3D fluorescence microscopy, wherein fluorescence emitters in a sample are repeatedly excited to emit fluorescence, and still images are produced of the sample by means of a microscope. The microscope has an imaging beam path with an optical resolution and a focal plane, wherein the fluorescence emitters are excited to emit fluorescence in such a manner that at least a subset of the fluorescence emitters is isolated in each still image so that the images of these fluorescence emitters can be separated in the still images within the optical resolution. The positions of the fluorescence emitters are localized in the generated still images, from the images of the isolated fluorescence emitters, with a location accuracy exceeding the optical resolution, and a high-resolution composite image is generated therefrom.
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公开(公告)号：US20170280076A1
公开(公告)日：2017-09-28
申请号：US15514453
申请日：2015-08-31
CPC分类号： H04N5/3572 , G02B21/0052 , G02B21/0076 , G02B21/06 , G02B21/082 , G02B21/361 , G02B21/365
摘要： The invention relates to a device and a method for imaging a sample (2) arranged in an object plane (1). Such a device comprises an optical relay system (3) which images an area of the sample (2) from the object plane (1) into an intermediate image plane (4). Here, the object plane (1) and the intermediate image plane (4) with an optical axis (5) of the relay system (3) include an angle different from 90°. The optical relay system (3) is composed of several lenses. The device also comprises an optical imaging system (6) with an objective, the optical axis (7) of which lies perpendicularly on the intermediate image plane (4) and which is focused on the intermediate image plane (4), with the result that the object plane (1) can be imaged undistorted onto a detector (8). Finally, the device also comprises an illumination apparatus (10) for illuminating the sample (2) with a light sheet (11), wherein the light sheet (11) lies essentially in the object plane (1) and defines an illumination direction, and wherein the normal of the object plane (1) defines a detection direction.In such a device, the object plane (1) with the optical axis (5) of the relay system (3) includes an angle, the value of which is smaller than the aperture angle of an object-side detection aperture cone (12) of the relay system (3), and the object plane (1) lies at least partially within the object-side detection aperture cone (12). The intermediate image plane (4) with the optical axis (5) of the relay system (3) also includes an angle, the value of which is smaller than the aperture angle of an intermediate image-side detection aperture cone (13) of the relay system (3), and the intermediate image plane (4) lies at least partially within the intermediate image-side detection aperture cone (13).
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公开(公告)号：US20170131534A1
公开(公告)日：2017-05-11
申请号：US15414689
申请日：2017-01-25
CPC分类号： G01N21/6458 , G01N2021/6463 , G01N2201/02 , G01N2201/06113 , G02B21/0032 , G02B21/0048 , G02B21/0076 , G02B21/008 , G02B21/06 , G02B21/16 , G02B21/26 , G02B21/367
摘要： A microscope including a sample carrier configured to support a sample. Excitation light illuminates the sample via an excitation beam path. Detection light from the sample is guided to detection means via a detection beam path. Through an objective arranged along the optical axis, excitation light is guided in direction of the sample carrier and detection light coming from the sample is guided in direction of the detection means. Beam-splitting means separate excitation light and detection light. Also provided are means for generating a light sheet from excitation light, and means for illuminating the sample with this light sheet. The light sheet lies in a plane at a nonzero angle to the optical axis. The means for illuminating the sample include an optical-deflecting device arranged on or at the sample carrier, which deflects excitation light from the objective into the plane of the light sheet via an optically active surface.
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公开(公告)号：US20170068080A1
公开(公告)日：2017-03-09
申请号：US15120162
申请日：2015-02-19
CPC分类号： G02B21/008 , G02B21/0032 , G02B21/0036 , G02B21/006 , G02B21/0076 , G02B21/16 , G02B21/365 , G02B27/0075 , H04N3/02 , H04N5/372
摘要： An arrangement for light sheet microscopy that includes a means for scanning a sample volume with a light sheet, which includes an angle δ≠90° with the optical axis of an objective. The light sheet passes through the entire sample volume in the propagation direction, and the depth of field Sobj of the objective is less than the optical-axis depth T of this sample volume. An optical device, disposed downstream of the objective, increases the depth of field Sobj to a depth of field Seff≧the depth T of this sample volume. The arrangement also includes a means for positioning the sample volume within the region of the depth of field Seff. A spatially resolving optoelectronic area sensor is disposed downstream of the optical device, and hardware and software are provided to generate sample-volume images from the electronic image signals output by the area sensor.
摘要翻译： 一种用于光板显微镜的装置，其包括用光板扫描样品体积的装置，该装置包括与物镜的光轴成δ≠90°的角度。 光片沿传播方向通过整个样本体积，物镜的景深Sobj小于该样本体积的光轴深度T. 设置在物镜下游的光学装置将场景Sobj的景深增加到该样本体积的深度S eff≥深度T. 该装置还包括用于将样本体积定位在景深Seff的区域内的装置。 空间分辨的光电区域传感器设置在光学装置的下游，并且提供硬件和软件以从由区域传感器输出的电子图像信号产生样本体积图像。
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