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    &agr;-amylase mutants
    4.
    发明授权
    &agr;-amylase mutants 有权
    α-淀粉酶突变体

    公开(公告)号：US06436888B1

    公开(公告)日：2002-08-20

    申请号：US09672459

    申请日：2000-09-28

    申请人： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgård-Frantzen

    发明人： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgård-Frantzen

    IPC分类号： C12N928

    CPC分类号： C12N9/2417 ,  C11D3/386 ,  C12P19/02 ,  C12P19/14

    摘要： The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.

    摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于亲本α-淀粉酶在下列特性中表现出至少一个变化：底物特异性，底物结合，底物切割 图案，热稳定性，pH /活性曲线，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。

    Starch debranching enzymes
    6.
    发明授权
    Starch debranching enzymes 失效
    淀粉脱支酶

    公开(公告)号：US07816113B2

    公开(公告)日：2010-10-19

    申请号：US12115123

    申请日：2008-05-05

    申请人： Henrik Bisgård-Frantzen ,  Allan Svendsen

    发明人： Henrik Bisgård-Frantzen ,  Allan Svendsen

    IPC分类号： C12N9/44 ,  C12N9/24 ,  C12N9/00 ,  C12P19/16 ,  C12P19/20 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12N9/2457 ,  C12N9/2451 ,  C12N9/246 ,  C12P19/16 ,  C12Y302/01041 ,  C12Y302/01068

    摘要： The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isoamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.

    摘要翻译： 本发明涉及母体淀粉脱支酶（即支链淀粉酶或异淀粉酶）的遗传改造变体，所述酶变体在与亲本酶相比在4-6的范围内具有改善的热稳定性和/或增加的活性 对于支链淀粉和/或糖原，与制备具有改进的热稳定性和/或改变的底物特异性的淀粉脱支酶变体的方法以及使用至少一种这样的酶变体将淀粉转化为一种或多种糖的方法 。

    Starch conversion process
    9.
    发明授权
    Starch conversion process 有权
    淀粉转化过程

    公开(公告)号：US06448049B1

    公开(公告)日：2002-09-10

    申请号：US09544123

    申请日：2000-04-06

    申请人： Noriko Tsutsumi ,  Henrik Bisgård-Frantzen ,  Allan Svendsen

    发明人： Noriko Tsutsumi ,  Henrik Bisgård-Frantzen ,  Allan Svendsen

    IPC分类号： C12N944

    CPC分类号： C12N9/246 ,  C12N9/2451 ,  C12P19/16 ,  C12Y302/01001 ,  C12Y302/01068

    摘要： The present invention relates to a starch conversion process of the type which includes a debranching step wherein an isoamylase being active at the process conditions prevailing is used for debranching the starch and to the use of thermostable isoamylases for starch conversion. The invention further relates to an isolated isoamylase obtained from a strain of the genus Rhodothermus and to cloned DNA sequences encoding isoamylases derived from a strain of Rhodothermus or Sulfolobus, to expression vectors comprising said DNA sequence, host cells comprising such expression vectors, and finally to methods for producing said isoamylases.

    摘要翻译： 本发明涉及一种淀粉转化方法，其包括脱支步骤，其中使用主要工艺条件的活性的异淀粉酶用于脱淀淀粉和使用热稳定的异戊酰化酶进行淀粉转化。 本发明还涉及从Rhodothermus菌株获得的分离的异淀粉酶，以及克隆编码来自Rhodothermus或Sulfolobus菌株的异戊酰化酶的DNA序列，包含所述DNA序列的表达载体，包含该表达载体的宿主细胞，最后到 生产所述异戊酰化酶的方法。

    α-amylase mutants
    10.
    发明授权
    α-amylase mutants 失效
    α-淀粉酶突变体

    公开(公告)号：US07115409B1

    公开(公告)日：2006-10-03

    申请号：US09327563

    申请日：1999-06-08

    申请人： Allan Svendsen ,  Henrik Bisgård-Frantzen ,  Torben Vedel Borchert

    发明人： Allan Svendsen ,  Henrik Bisgård-Frantzen ,  Torben Vedel Borchert

    IPC分类号： C12N9/26 ,  C12N9/28 ,  C07H21/04

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprises i) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like α-amylase variant with respect to the property in question.

    摘要翻译： 本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体具有与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关的相关性 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得的类戊酰样α-淀粉酶变体。