公开(公告)号：US20240191306A1

公开(公告)日：2024-06-13

申请号：US18430156

申请日：2024-02-01

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Helen Moinova ,  Amitabh Chak ,  Joseph Willis ,  Thomas LaFramboise

IPC: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6853 ,  G01N33/574

CPC classification number: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6853 ,  G01N33/574 ,  C12Q2600/154 ,  C12Q2600/156

Abstract: The disclosure provides methods for identifying genomic loci (e.g., vimentin and/or SqBE18) that are differentially methylated in metaplasias (e.g., Barrett's esophagus) and/or neoplastic cancers (e.g., esophageal cancers). Identification of methylated genomic loci has numerous uses, including for example, to characterize disease risk, to predict responsiveness to therapy, to non-invasively diagnose subjects and to treat subjects determined to have gastrointestinal metaplasias and/or neoplasias.

公开(公告)号：US20190261962A1

公开(公告)日：2019-08-29

申请号：US16405634

申请日：2019-05-07

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： Sanford Markowitz ,  Amitabh Chak ,  Joseph Willis ,  Jeffrey Silver Taggart ,  Rebecca Blice ,  Dean Secrest ,  Dennis Seidlak

IPC: A61B10/02

Abstract: A device for collecting a biological sample in a patient includes a collection portion having a first axial end portion and a second axial end portion. The second axial end portion has a collapsed position and an expanded position. The second axial end portion moves in an axial direction relative to the first axial end portion when the second axial end portion moves between the collapsed position and the expanded position. The second axial end portion extends axially into the first axial end portion and has a concave shape when in the collapsed position. The second axial end portion is convex when in the expanded position.

公开(公告)号：US09580754B2

公开(公告)日：2017-02-28

申请号：US13670155

申请日：2012-11-06

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Joseph Willis ,  Amitabh Chak ,  Rom Leidner

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6886 ,  C07H21/04 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/136 ,  C12Q2600/154 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as neoplasia of the upper or lower gastrointestinal tract, pancreas, and/or bladder. Nucleic acid sequences for regions of vimentin that are found to be differentially methylated are described. The present disclosure further describes bisulfite-converted vimentin template DNA sequences. Oligonucleotide primer sequences for use in assays (e.g., methylation-sensitive PCR assays or HpaII assays) designed to detect the methylation status of the vimentin gene are also described.

Abstract translation: 本申请描述了用于检测和治疗波形蛋白相关瘤形成的方法和组合物。 已经观察到波形蛋白核苷酸序列的差异甲基化在波形蛋白相关瘤形成中，例如上，下胃肠道，胰腺和/或膀胱的瘤形成。 描述了发现被差异甲基化的波形蛋白区域的核酸序列。 本公开进一步描述了亚硫酸氢盐转化的波形蛋白模板DNA序列。 还描述了用于检测波形蛋白基因的甲基化状态的测定中的寡核苷酸引物序列（例如，甲基化敏感的PCR测定或HpaII测定）。

公开(公告)号：US20220096064A1

公开(公告)日：2022-03-31

申请号：US17643341

申请日：2021-12-08

Applicant: Case Western Reserve University

Inventor： Sanford Markowitz ,  Amitabh Chak ,  Joseph Willis ,  Jeffrey Silver Taggart ,  Rebecca Blice ,  Dean Secrest ,  Dennis Siedlak

IPC: A61B10/02

Abstract: A device for collecting a biological sample in a patient includes a collection portion having a first axial end portion and a second axial end portion. The second axial end portion has a collapsed position and an expanded position. The second axial end portion moves in an axial direction relative to the first axial end portion when the second axial end portion moves between the collapsed position and the expanded position. The second axial end portion extends axially into the first axial end portion and has a concave shape when in the collapsed position. The second axial end portion is convex when in the expanded position.

公开(公告)号：US20220096062A1

公开(公告)日：2022-03-31

申请号：US17643329

申请日：2021-12-08

Applicant: Case Western Reserve University

Inventor： Sanford Markowitz ,  Amitabh Chak ,  Joseph Willis ,  Jeffrey Silver Taggart ,  Rebecca Blice ,  Dean Secrest ,  Dennis Siedlak

IPC: A61B10/02

Abstract: A device for collecting a biological sample in a patient includes a collection portion having a first axial end portion and a second axial end portion. The second axial end portion has a collapsed position and an expanded position. The second axial end portion moves in an axial direction relative to the first axial end portion when the second axial end portion moves between the collapsed position and the expanded position. The second axial end portion extends axially into the first axial end portion and has a concave shape when in the collapsed position. The second axial end portion is convex when in the expanded position.

公开(公告)号：US11136629B2

公开(公告)日：2021-10-05

申请号：US16564392

申请日：2019-09-09

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Joseph Willis ,  Amitabh Chak ,  Rom Leidner

IPC: C12Q1/68 ,  C12Q1/6886 ,  C07H21/04

Abstract: This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as neoplasia of the upper or lower gastrointestinal tract, pancreas, and/or bladder.

公开(公告)号：US10450615B2

公开(公告)日：2019-10-22

申请号：US16388029

申请日：2019-04-18

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Joseph Willis ,  Amitabh Chak ,  Rom Leidner

IPC: C12Q1/68 ,  C12Q1/6886 ,  C07H21/04

Abstract: This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as neoplasia of the upper or lower gastrointestinal tract, pancreas, and/or bladder.

公开(公告)号：US20190136325A1

公开(公告)日：2019-05-09

申请号：US15540956

申请日：2015-12-30

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Joseph E. Willis ,  Helen Moinova ,  Thomas LaFramboise ,  Omar de la Cruz Cabrera ,  Amitabh Chak

IPC: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6837

Abstract: The disclosure provides methods for identifying genomic loci that are differentially/methylated in neoplastic cancers, e.g., esophageal cancers. Identification of methylated genomic loci, and optionally in combination with the identification of somatic mutations in TP53, has numerous uses, including for example, to characterize disease risk, to predict responsiveness to therapy, to non-invasively diagnose subjects and to treat subjects determined to have gastrointestinal neoplasias.

公开(公告)号：US12262879B2

公开(公告)日：2025-04-01

申请号：US16902579

申请日：2020-06-16

Applicant: Case Western Reserve University

Inventor： Amitabh Chak ,  Sanford Markowitz ,  Dennis Siedlak ,  Joseph Willis ,  Lishan Aklog ,  Michael Boutillette ,  Richard Yazbeck

IPC: A61B10/02 ,  A61B10/04 ,  A61M25/10

Abstract: A device for collecting a biological sample in a subject, the device including an inflatable portion attached to a tubular member, and designed to expand from within the tubular member when inflated and to retract within the tubular member, a surface on the inflatable portion provided with varied thickness to facilitate movement of the inflatable portion between an expanded state and a retracted state, and a plurality of projections on the surface to allow collection of the biological sample when the inflatable portion is in the expanded state.

公开(公告)号：US12227810B2

公开(公告)日：2025-02-18

申请号：US18430156

申请日：2024-02-01

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Helen Moinova ,  Amitabh Chak ,  Joseph Willis ,  Thomas LaFramboise

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/6886 ,  G01N33/574

Abstract: The disclosure provides methods for identifying genomic loci (e.g., vimentin and/or SqBE18) that are differentially methylated in metaplasias (e.g., Barrett's esophagus) and/or neoplastic cancers (e.g., esophageal cancers). Identification of methylated genomic loci has numerous uses, including for example, to characterize disease risk, to predict responsiveness to therapy, to non-invasively diagnose subjects and to treat subjects determined to have gastrointestinal metaplasias and/or neoplasias.