公开(公告)号：US11845955B2

公开(公告)日：2023-12-19

申请号：US17878438

申请日：2022-08-01

申请人： Case Western Reserve University ,  UNIVERSITY HOSPITALS CLEVELAND MEDICAL CENTER

发明人： Michael W. Jenkins ,  Yehe Liu

IPC分类号： G06T7/136 ,  C12N5/00 ,  G06T7/11 ,  C12N5/071 ,  G01N21/47 ,  G01N21/49 ,  G01N33/483 ,  G06T5/00 ,  G06T5/20 ,  G06T5/50 ,  G06T7/00

CPC分类号： C12N5/0018 ,  C12N5/0679 ,  C12N5/0691 ,  G01N21/4795 ,  G01N21/49 ,  G01N33/4833 ,  G06T5/002 ,  G06T5/009 ,  G06T5/20 ,  G06T5/50 ,  G06T7/0012 ,  G06T7/11 ,  G06T7/136 ,  C12N2513/00 ,  G06T2207/10101 ,  G06T2207/20224 ,  G06T2207/30024

摘要： The present disclosure relates to a simple, fast, and low cost method for 3D microvascular imaging, termed “scatter labeled imaging of microvasculature in excised tissue” (SLIME). The method can include perfusing a contrast agent through vasculature of a tissue sample. The contrast agent can include colloids and a dispersant. After the contrast agent is perfused through the vasculature, the vasculature of the tissue sample can be treated with a molecule that cross links with at least a portion of the dispersant to form a sticky, non-Newtonian polymer that prevents leakage of the contrast agent out of the vasculature of the tissue sample. The tissue sample can then be immersed in a solution comprising a clearing agent and subsequently imaged.

公开(公告)号：US11427801B2

公开(公告)日：2022-08-30

申请号：US16761278

申请日：2018-11-09

申请人： Case Western Reserve University ,  UNIVERSITY HOSPITALS CLEVELAND MEDICAL CENTER

发明人： Michael W. Jenkins ,  Yehe Liu

IPC分类号： G06T7/136 ,  C12N5/00 ,  G06T7/11 ,  C12N5/071 ,  G01N21/47 ,  G01N21/49 ,  G01N33/483 ,  G06T5/00 ,  G06T5/20 ,  G06T5/50 ,  G06T7/00

摘要： The present disclosure relates to a simple, fast, and low cost method for 3D microvascular imaging, termed “scatter labeled imaging of microvasculature in excised tissue” (SLIME). The method can include perfusing a contrast agent through vasculature of a tissue sample with a contrast perfusing unit (22). The contrast agent can include colloids and a dispersant. After the contrast agent is perfused through the vasculature, the vasculature of the tissue sample can be treated with a cross-linking agent delivery unit (24) providing a molecule that cross links with at least a portion of the dispersant to form a sticky, non-Newtonian polymer that prevents leakage of the contrast agent out of the vasculature of the tissue sample. The tissue sample can then be immersed in a solution comprising a clearing agent with an optical clearing unit (26) and subsequently imaged.