公开(公告)号：US08137944B2

公开(公告)日：2012-03-20

申请号：US11887977

申请日：2005-07-07

申请人： Casper Tune Berg ,  Patrick M. F. Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sørensen ,  Charlotte Refdahl Thoudahl

发明人： Casper Tune Berg ,  Patrick M. F. Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sørensen ,  Charlotte Refdahl Thoudahl

IPC分类号： C12N9/28 ,  C12N9/26 ,  C12N9/24 ,  C12N9/00 ,  C12P21/06 ,  A21D2/00 ,  A23C17/00 ,  A23L1/08 ,  A23K3/00 ,  A23K3/08 ,  C07H21/04 ,  C07K1/00

CPC分类号： C12Y302/0106 ,  A21D8/042 ,  A23K20/189 ,  A23L7/107 ,  A23L29/35 ,  A23V2002/00 ,  C12N9/2417 ,  A23V2300/21

摘要： Variant polypeptides derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the variant polypeptides comprise an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophila exoamylase sequence shown as SEQ ID NO: 1.

摘要翻译： 衍生自具有非麦芽糖外切酶活性的亲本多肽的变体多肽，其中所述变体多肽在选自以下的一个或多个位置包含氨基酸突变：121,161,223,146,157,158,199， 229,303,306,309,316,353,26,70,105,188,272,339，参考如SEQ ID NO：1所示的糖酵母假单胞菌外切酶序列的位置编号。

公开(公告)号：US20090214706A1

公开(公告)日：2009-08-27

申请号：US11887977

申请日：2005-07-07

申请人： Casper Tune Berg ,  Patrick M.F. Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sørensen ,  Charlotte Refdahl Thoudahl

发明人： Casper Tune Berg ,  Patrick M.F. Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sørensen ,  Charlotte Refdahl Thoudahl

IPC分类号： A21D2/26 ,  C12N9/28 ,  A21D13/06 ,  C12N15/56 ,  C12N15/74 ,  C12N1/21 ,  A23K1/165

CPC分类号： C12Y302/0106 ,  A21D8/042 ,  A23K20/189 ,  A23L7/107 ,  A23L29/35 ,  A23V2002/00 ,  C12N9/2417 ,  A23V2300/21

摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.

摘要翻译： 我们描述了可从具有非麦芽糖苷外切酶活性的亲本多肽衍生的PS4变体多肽，其中PS4变体多肽在选自以下的一个或多个位置包含氨基酸突变：121,161,223,146,157 ，158,198,229,303,306,309,316,353,26,70,205,188,272,339，参考SEQ ID NO：1所示的假单胞菌属的嗜酸芽胞弧菌外切酶序列的位置编号。

公开(公告)号：US08030050B2

公开(公告)日：2011-10-04

申请号：US11534624

申请日：2006-09-22

申请人： Casper Tune Berg ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sørensen ,  Charlotte Refdahl Thoudahl

发明人： Casper Tune Berg ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sørensen ,  Charlotte Refdahl Thoudahl

IPC分类号： C12N9/28 ,  C12N9/26 ,  C12N9/24 ,  C12N9/00 ,  C12P21/06 ,  A21D2/00 ,  A23C9/12 ,  C07H21/04

CPC分类号： A21D8/042 ,  A23K20/147 ,  A23K50/30 ,  A23K50/75 ,  A23L33/18 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2425 ,  C12N9/2428

摘要： The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

摘要翻译： 本发明描述了可衍生自具有选自以下的淀粉酶活性的亲本多肽的PS4变体多肽： 161,178,179,223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参照SEQ ID NO：1所示的假单胞菌属酵母胞外淀粉酶序列的位置编号，这种多肽作为食品或饲料添加剂的使用，以及编码这样的核酸。

公开(公告)号：US20080227173A1

公开(公告)日：2008-09-18

申请号：US11534624

申请日：2006-09-22

申请人： Casper Tune BERG ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sorensen ,  Charlotte Refdahl Thoudahl

发明人： Casper Tune BERG ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sorensen ,  Charlotte Refdahl Thoudahl

IPC分类号： C12N9/28

CPC分类号： A21D8/042 ,  A23K20/147 ,  A23K50/30 ,  A23K50/75 ,  A23L33/18 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2425 ,  C12N9/2428

摘要： The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

摘要翻译： 本发明描述了可衍生自具有选自以下的淀粉酶活性的亲本多肽的PS4变体多肽： 161,178,179,223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参照SEQ ID NO：1所示的假单胞菌属酵母胞外淀粉酶序列的位置编号，这种多肽作为食品或饲料添加剂的使用，以及编码这样的核酸。

公开(公告)号：US20080292747A1

公开(公告)日：2008-11-27

申请号：US11970473

申请日：2008-01-07

申请人： Casper Tune Berg ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sorensen ,  Charlotte Refdahl Thoudahl

发明人： Casper Tune Berg ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sorensen ,  Charlotte Refdahl Thoudahl

IPC分类号： A21D2/08 ,  A21D13/06 ,  C12N9/28 ,  C12P19/00

CPC分类号： A21D8/042 ,  A23L33/18 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2425 ,  C12N9/2428

摘要： Described is a PS4 variant polypeptide derivable from a polypeptide having amylase activity selected from: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; referring to the numbering of a Pseudomonas saccharophilia exoamylase shown as SEQ ID NO: 1.

摘要翻译： 描述了可衍生自具有选自以下的淀粉酶活性的多肽的PS4变体多肽： 223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 指的是SEQ ID NO：1所示的假单胞菌糖酵母外切酶的编号。