-
1.发明授权Apparatus for mixing liquids used in countercurrent multiphase liquid separation 失效用于混合逆流多相液体分离中使用的液体的装置
公开(公告)号：US5578201A
公开(公告)日：1996-11-26
申请号：US451353
申请日：1995-05-26
申请人： Charles F. Collier , Brian A. Deane , William E. Feathers , Robert A. King , Alexander D. Kopatsis , Thomas P. Ladisch , Peter M. Mrozinski , Norman P. Pih
发明人： Charles F. Collier , Brian A. Deane , William E. Feathers , Robert A. King , Alexander D. Kopatsis , Thomas P. Ladisch , Peter M. Mrozinski , Norman P. Pih
CPC分类号： C12M47/04 , B01D11/04 , G01N1/34 , G01N2001/4061
摘要： A reusable apparatus for multiphase, multistage countercurrent liquid separation is presented. The apparatus is particular useful in progressive multistage separation of target microorganisms from background organisms and materials with precise transfer, air exclusion, homogeneous mixing, isolation from the ambient atmosphere, in the absence of valves and at ow cost. The structure has at least one chamber in which a piston-like sliding bottom is sealingly moved or braked and includes internal structure for mixing, preferably a magnetic-stainless steel ball which can be reciprocated from the outside to mix the liquid contents. Motion of the bottom is limited by stops. A number of ways to actuate the apparatus are taught.
摘要翻译： 提出了一种用于多相，多级逆流液体分离的可重复使用的设备。 该设备特别适用于目标微生物与背景生物体和具有精确转移，空气排除，均匀混合，在不存在阀门的情况下以及成本低的情况下与环境大气隔离的材料的进行多级分离。 该结构具有至少一个室，其中活塞状滑动底部被密封地移动或制动，并且包括用于混合的内部结构，优选地是磁性不锈钢球，其可以从外部往复运动以混合液体内容物。 底部的运动受到停止的限制。 教授了许多致动装置的方法。
-
2.发明授权
公开(公告)号：US5433837A
公开(公告)日：1995-07-18
申请号：US113480
申请日：1993-08-27
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44704 , G01N27/44717
摘要： A gel cassette useful in electrophoretic separation of molecular fragments is disclosed, in which a gelatinous substrate is uniquely configured to concentrate similar separated fragments. Containment means is also disclosed which provides for the desired configuration of the substrate. The containment means further includes a cover design which locks into an open position to eliminate cross contamination. In addition, processes for the preparation of such cassettes and for use of the cassettes in separation and transfer apparatus are disclosed.
摘要翻译： 公开了一种可用于电泳分离分子片段的凝胶盒，其中凝胶状底物被独特地构造成浓缩相似的分离碎片。 还公开了提供基板的期望构造的容纳装置。 容纳装置还包括锁定到打开位置以消除交叉污染的盖设计。 此外，公开了用于制备这种盒和用于分离和转印装置中的盒的方法。
-
公开(公告)号：US5104231A
公开(公告)日：1992-04-14
申请号：US736177
申请日：1991-07-26
申请人： Charles F. Collier , James D. Riall
发明人： Charles F. Collier , James D. Riall
CPC分类号： G01N35/02 , B01F11/0037 , B01F15/00435 , Y10T74/18544
摘要： An apparatus for providing linear motion in two opposed directions and circular motion in a first direction using a single drive motor.
摘要翻译： 用于使用单个驱动马达在第一方向上以两个相对的方向提供线性运动和圆周运动的装置。
-
4.发明授权
公开(公告)号：US5474663A
公开(公告)日：1995-12-12
申请号：US113538
申请日：1993-08-27
IPC分类号： G01N33/50 , G01N27/447 , G01N33/68 , G01N27/26
CPC分类号： G01N27/44717
摘要： A bowed framed membrane is disclosed for use in automated direct blot electrophoresis and subsequent membrane process steps to develop a chemiluminescent image of the resultant blot of selected fragments and to detect that image. A frame made from a thin rigid plastic plate such as polyethylene is provided with a window. A membrane covers that window and is bonded on two opposing sides only while the frame is held in a bowed condition. The membrane is maintained under tension during processing by the bowing permitting processing at the elevated temperatures used in molecular biological procedures without loss of flatness. Processes for preparing such frames and various utilities are also disclosed.
摘要翻译： 公开了一种弓形框膜，用于自动化直接印迹电泳和随后的膜工艺步骤，以开发所选片段的所得印迹的化学发光图像并检测该图像。 由诸如聚乙烯的薄刚性塑料板制成的框架设置有窗口。 只有当框架保持在鞠躬状态时，膜才能覆盖该窗户并且仅在两个相对的侧面上结合。 通过在分子生物学程序中使用的升高的温度下进行弯曲允许加工，膜在保持张力的同时不损失平坦度。 还公开了制备这种框架和各种实用程序的方法。
-
公开(公告)号：US5130254A
公开(公告)日：1992-07-14
申请号：US528357
申请日：1990-05-25
CPC分类号： G01N35/1079 , Y10T436/119163 , Y10T436/2575
摘要： A probe is introduced through the elastomeric cap of a closed sample container and moved sideways to stretch the cap and thereby provide an air passage to atmosphere while the sample is aspirated.
摘要翻译： 探针通过封闭的样品容器的弹性盖引入，并且侧向移动以拉伸帽，从而在抽吸样品时提供通向大气的空气通道。
-
6.发明授权
公开(公告)号：US5512146A
公开(公告)日：1996-04-30
申请号：US426324
申请日：1995-04-21
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44704 , G01N27/44717
摘要： A gel cassette useful in electrophoretic separation of molecular fragments is disclosed, in which a gelatinous substrate is uniquely configured to concentrate similar separated fragments. Containment means is also disclosed which provides for the desired configuration of the substrate. The containment means further includes a cover design which locks into an open position to eliminate cross contamination. In addition, processes for the preparation of such cassettes and for use of the cassettes in separation and transfer apparatus are disclosed.
-
7.发明授权
公开(公告)号：US5234559A
公开(公告)日：1993-08-10
申请号：US945553
申请日：1992-09-16
IPC分类号： G01N27/447
CPC分类号： G01N27/44717
摘要： A separation and direct blot transfer module useful in gel electrophoresis is disclosed in which a transfer membrane stabilized by a frame is moved substantially orthogonally relative to the plane of the separation gel while in minimal contact with the end of the gel toward which molecular fragments are driven electrophoretically. The frame moves at a controlled velocity in a selected direction away from a line of contact that generates a controlled tension in the membrane which is a function of the loading on the line of contact. The module is particularly amenable to automated processing. Apparatus for automated separation, transfer, membrane processing, detection and identification is disclosed in which the module of the invention is employed.
摘要翻译： 公开了一种用于凝胶电泳的分离和直接印迹转移模块，其中通过框架稳定的转移膜相对于分离凝胶的平面基本上正交地移动，同时与分子片段被驱动的凝胶末端接触最小 电泳。 框架以受控制的速度沿远离接触线的选定方向移动，该线产生膜中的受控张力，其是接触线上的负载的函数。 该模块特别适合于自动化处理。 公开了使用本发明的模块的自动分离，转移，膜处理，检测和识别的装置。
-
-
-
-
-
-
搜索结果
7 条记录 (耗时 0.032 秒)
