公开(公告)号：US20080108121A1

公开(公告)日：2008-05-08

申请号：US11797418

申请日：2007-05-03

申请人： Cheng-Hsien Liu ,  Hwan-You Chang ,  Long Hsu ,  Chen-Ta Ho ,  Ruei-Zeng Lin ,  Wai William Wang ,  Ta-Yuan Lee ,  Chung-Cheng Chou ,  Chen Peng ,  Fung-Hsu Wu

发明人： Cheng-Hsien Liu ,  Hwan-You Chang ,  Long Hsu ,  Chen-Ta Ho ,  Ruei-Zeng Lin ,  Wai William Wang ,  Ta-Yuan Lee ,  Chung-Cheng Chou ,  Chen Peng ,  Fung-Hsu Wu

IPC分类号： C12N13/00 ,  C12M1/00

CPC分类号： C12N5/0697 ,  C12N5/067 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2529/00 ,  C12N2535/10

摘要： A method of arranging cells comprises: (a) applying a voltage to two electrodes so as to allow a plurality of cells suspended in a dielectrophoresis-manipulating buffer (DEP-manipulating buffer) to be driven to be arranged into a pattern; (b) replacing the DEP-manipulating buffer with a solution comprising calcium ion and magnesium ion which helps the patterned cells adhere to the substrate; and (c) replacing the solution comprising calcium ion and magnesium ion with a medium so as to allow the patterned cells to grow on the substrate.

摘要翻译： 一种布置电池的方法包括：（a）向两个电极施加电压，以允许驱动悬浮在介电电泳操纵缓冲器（DEP-操纵缓冲器）中的多个电池被布置成图案; （b）用包含钙离子和镁离子的溶液代替DEP操纵缓冲液，其帮助图案化细胞粘附到基底上; 和（c）用培养基替换包含钙离子和镁离子的溶液，以使图案化细胞在基底上生长。

公开(公告)号：US07838819B2

公开(公告)日：2010-11-23

申请号：US12019316

申请日：2008-01-24

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  Wai William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  Wai William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： H01S1/00 ,  H01S3/00 ,  H05H3/02

CPC分类号： G02B21/32 ,  G02B5/1842 ,  G02B21/0088

摘要： An apparatus of generating optical tweezers with momentum is provided for providing optical tweezers with a first momentum on a test piece. The apparatus comprises a laser source, a diffractive optical element (DOE) and a lens. The laser source is for outputting a laser beam. The DOE has a first phase-delay picture, and the laser beam forms a diffraction pattern after passing the first phase-delay picture. The lens is for receiving and focusing the diffraction pattern on the test piece to form the first optical tweezers with the first momentum. The lens has an optical axis intersecting the DOE at an optical intersection point, a geometric center of the phase-delay picture has a displacement vector relative to the optical intersection point and a direction of the first momentum is related to a direction of the displacement vector.

摘要翻译： 提供产生具有动量的光学镊子的装置，用于在测试片上提供具有第一动量的光学镊子。 该装置包括激光源，衍射光学元件（DOE）和透镜。 激光源用于输出激光束。 DOE具有第一相位延迟图像，并且激光束在通过第一相位延迟图像之后形成衍射图案。 透镜用于接收和聚焦在测试片上的衍射图案以形成具有第一动量的第一光学镊子。 透镜在光学交点处具有与DOE相交的光轴，相位延迟图像的几何中心具有相对于光学交叉点的位移矢量，并且第一动量的方向与位移矢量的方向相关 。

公开(公告)号：US20080174870A1

公开(公告)日：2008-07-24

申请号：US12019316

申请日：2008-01-24

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  Wai William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  Wai William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： G02B27/42

CPC分类号： G02B21/32 ,  G02B5/1842 ,  G02B21/0088

摘要： An apparatus of generating optical tweezers with momentum is provided for providing optical tweezers with a first momentum on a test piece. The apparatus comprises a laser source, a diffractive optical element (DOE) and a lens. The laser source is for outputting a laser beam. The DOE has a first phase-delay picture, and the laser beam forms a diffraction pattern after passing the first phase-delay picture. The lens is for receiving and focusing the diffraction pattern on the test piece to form the first optical tweezers with the first momentum. The lens has an optical axis intersecting the DOE at an optical intersection point, a geometric center of the phase-delay picture has a displacement vector relative to the optical intersection point and a direction of the first momentum is related to a direction of the displacement vector.

摘要翻译： 提供产生具有动量的光学镊子的装置，用于在测试片上提供具有第一动量的光学镊子。 该装置包括激光源，衍射光学元件（DOE）和透镜。 激光源用于输出激光束。 DOE具有第一相位延迟图像，并且激光束在通过第一相位延迟图像之后形成衍射图案。 透镜用于接收和聚焦在测试片上的衍射图案以形成具有第一动量的第一光学镊子。 透镜在光学交点处具有与DOE相交的光轴，相位延迟图像的几何中心具有相对于光学交叉点的位移矢量，并且第一动量的方向与位移矢量的方向相关 。

公开(公告)号：US20090052038A1

公开(公告)日：2009-02-26

申请号：US12222250

申请日：2008-08-06

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： G02B27/42

CPC分类号： G02B21/32

摘要： An apparatus and a method for changing optical tweezers are provided. The apparatus includes a diffractive optical element (DOE), a mask unit and an objective lens. The DOE includes a plurality of phase delay patterns. The mask unit includes a plurality of mask patterns that correspond to the phase delay patterns, respectively, wherein at least a portion of the mask patterns are complementary. A laser beam passing through each phase diffractive pattern correspondingly passes through each mask pattern to generate a compound diffractive pattern. The objective lens receives the compound diffractive pattern and focuses it on an examining object to form an optical tweezers.

摘要翻译： 提供了一种用于改变光学镊子的设备和方法。 该装置包括衍射光学元件（DOE），掩模单元和物镜。 DOE包括多个相位延迟模式。 掩模单元包括分别对应于相位延迟图案的多个掩模图案，其中至少一部分掩模图案是互补的。 通过每个相位衍射图案的激光束相应地通过每个掩模图案以产生复合衍射图案。 物镜接收复合衍射图案并将其聚焦在检查对象上以形成光学镊子。

公开(公告)号：US08258461B2

公开(公告)日：2012-09-04

申请号：US12219871

申请日：2008-07-30

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  William Wang ,  Chung-Cheng Chou ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  William Wang ,  Chung-Cheng Chou ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： B01D21/01

CPC分类号： G02B21/32 ,  Y10T436/25375

摘要： An apparatus of generating an optical tweezers with momentum and method thereof and an optical tweezers photo-image for guiding particles are provided. The apparatus generates at least one optical tweezers on an examined object that carries at least one particle. The apparatus includes a laser source, a diffractive optical element and a convergent lens. The laser beam from the laser source passes through the diffractive optical element to produce a diffractive pattern. The laser beam is then received by the convergent lens and then to be focused on a plane of the examined object. The optic axis of the convergent lens is substantially not perpendicular to the plane of the examined object, so that the laser beam is projected onto the plane of the examined object in a skewed manner for providing a lateral momentum to move the particle.

摘要翻译： 提供了一种用于产生具有动量及其方法的光学镊子的装置和用于引导颗粒的光学镊子照片。 该装置在携带至少一个颗粒的被检查物体上产生至少一个光学镊子。 该装置包括激光源，衍射光学元件和会聚透镜。 来自激光源的激光束通过衍射光学元件以产生衍射图案。 然后激光束被会聚透镜接收，然后被聚焦在被检查物体的平面上。 会聚透镜的光轴基本上不垂直于被检查物体的平面，使得激光束以倾斜的方式投影到被检查物体的平面上，以提供横向动量来移动颗粒。

公开(公告)号：US07804058B2

公开(公告)日：2010-09-28

申请号：US12222249

申请日：2008-08-06

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Ai-Tang Chang ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Ai-Tang Chang ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： H01S1/00 ,  H01S3/00 ,  H05H3/02

CPC分类号： G02B21/32

摘要： An optical tweezers controlling device including a light source, an objective lens and a focus adjusting unit is provided. The focus adjusting unit disposed between the light source and the objective lens includes a mirror set and a zoom lens set. The mirror set has at least a mirror. The mirror is rotatable such that after a light of the light source is projected to the mirror, the reflective direction of the light reflected from the mirror is changeable. The zoom lens set has at least a zoom lens disposed in accordance with the mirror. By rotating the mirror or changing the focal length of the zoom lens, the focusing location of the light changes on the focal plane of the objective lens or in the front or the rear of the focal plane.

摘要翻译： 提供一种包括光源，物镜和焦点调节单元的光学镊子控制装置。 设置在光源和物镜之间的聚焦调节单元包括镜组和变焦透镜组。 镜子至少有一个镜子。 反射镜可旋转，使得在光源的光被投射到反射镜之后，从反射镜反射的光的反射方向是可变的。 变焦透镜组具有至少根据镜子设置的变焦透镜。 通过旋转镜子或改变变焦镜头的焦距，光的聚焦位置在物镜的焦平面或焦平面的前面或后面改变。

公开(公告)号：US07786432B2

公开(公告)日：2010-08-31

申请号：US12222250

申请日：2008-08-06

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： H01S1/00 ,  H01S3/00 ,  H05H3/02

CPC分类号： G02B21/32

摘要： An apparatus and a method for changing optical tweezers are provided. The apparatus includes a diffractive optical element (DOE), a mask unit and an objective lens. The DOE includes a plurality of phase delay patterns. The mask unit includes a plurality of mask patterns that correspond to the phase delay patterns, respectively, wherein at least a portion of the mask patterns are complementary. A laser beam passing through each phase diffractive pattern correspondingly passes through each mask pattern to generate a compound diffractive pattern. The objective lens receives the compound diffractive pattern and focuses it on an examining object to form an optical tweezers.

摘要翻译： 提供了一种用于改变光学镊子的设备和方法。 该装置包括衍射光学元件（DOE），掩模单元和物镜。 DOE包括多个相位延迟模式。 掩模单元包括分别对应于相位延迟图案的多个掩模图案，其中至少一部分掩模图案是互补的。 通过每个相位衍射图案的激光束相应地通过每个掩模图案以产生复合衍射图案。 物镜接收复合衍射图案并将其聚焦在检查对象上以形成光学镊子。

公开(公告)号：US20090051999A1

公开(公告)日：2009-02-26

申请号：US12222249

申请日：2008-08-06

申请人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Ai-Tang Chang ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

发明人： Long Hsu ,  Cheng-Hsien Liu ,  Sheng-Yang Tseng ,  Ai-Tang Chang ,  Chung-Cheng Chou ,  William Wang ,  Fung-Hsu Wu ,  Chen Peng ,  Ta-Yuan Lee

IPC分类号： G02B26/08

CPC分类号： G02B21/32

摘要： An optical tweezers controlling device including a light source, an objective lens and a focus adjusting unit is provided. The focus adjusting unit disposed between the light source and the objective lens includes a mirror set and a zoom lens set. The mirror set has at least a mirror. The mirror is rotatable such that after a light of the light source is projected to the mirror, the reflective direction of the light reflected from the mirror is changeable. The zoom lens set has at least a zoom lens disposed in accordance with the mirror. By rotating the mirror or changing the focal length of the zoom lens, the focusing location of the light changes on the focal plane of the objective lens or in the front or the rear of the focal plane.

摘要翻译： 提供一种包括光源，物镜和焦点调节单元的光学镊子控制装置。 设置在光源和物镜之间的聚焦调节单元包括镜组和变焦透镜组。 镜子至少有一个镜子。 反射镜可旋转，使得在光源的光被投射到反射镜之后，从反射镜反射的光的反射方向是可变的。 变焦透镜组具有至少根据镜子设置的变焦透镜。 通过旋转镜子或改变变焦镜头的焦距，光的聚焦位置在物镜的焦平面或焦平面的前面或后面改变。

公开(公告)号：US07829839B2

公开(公告)日：2010-11-09

申请号：US12285958

申请日：2008-10-17

申请人： Cheng-Hsien Liu ,  William Wang ,  Long Hsu ,  Chung-Cheng Chou ,  Sheng-Yang Tseng ,  Chen Peng ,  Fung-Hsu Wu ,  Ta-Yuan Lee

发明人： Cheng-Hsien Liu ,  William Wang ,  Long Hsu ,  Chung-Cheng Chou ,  Sheng-Yang Tseng ,  Chen Peng ,  Fung-Hsu Wu ,  Ta-Yuan Lee

IPC分类号： H05H3/00 ,  C25B9/16

CPC分类号： B03C5/026 ,  G01N35/10 ,  G01N2035/1046

摘要： An optical tweezers lifting apparatus is provided. The optical tweezers lifting apparatus includes an optical tweezers and a particle-lifting device. The particle-lifting device includes a substrate and a plurality of electrodes that are disposed on the bottom of a flow path in the substrate. When a dielectrophoresis (DEP) solution with a plurality of floating particles is conducted into the flow path and upon those electrodes and a voltage is applied to these electrodes, these particles would be driven by a negative DEP force to move upward to a specific depth in the flow path. Meanwhile, the optical tweezers of the apparatus is selectively focused at the specific depth in the flow path.

摘要翻译： 提供一种光学镊子提升装置。 光学镊子提升装置包括光学镊子和颗粒提升装置。 颗粒提升装置包括基板和设置在基板中的流路底部的多个电极。 当具有多个浮动颗粒的介电电泳（DEP）溶液被引导到流动路径中并且在那些电极上并且向这些电极施加电压时，这些颗粒将被负的DEP力驱动以向上移动到特定的深度 流路。 同时，该设备的光学镊子选择性地聚焦在流路中的特定深度处。

公开(公告)号：US20100108872A1

公开(公告)日：2010-05-06

申请号：US12285958

申请日：2008-10-17

申请人： Cheng-Hsien Liu ,  William Wang ,  Long Hsu ,  Chung-Cheng Chou ,  Sheng-Yang Tseng ,  Chen Peng ,  Fung-Hsu Wu ,  Ta-Yuan Lee

发明人： Cheng-Hsien Liu ,  William Wang ,  Long Hsu ,  Chung-Cheng Chou ,  Sheng-Yang Tseng ,  Chen Peng ,  Fung-Hsu Wu ,  Ta-Yuan Lee

IPC分类号： H05H3/00 ,  C25B9/16

CPC分类号： B03C5/026 ,  G01N35/10 ,  G01N2035/1046

摘要： An optical tweezers lifting apparatus is provided. The optical tweezers lifting apparatus includes an optical tweezers and a particle-lifting device. The particle-lifting device includes a substrate and a plurality of electrodes that are disposed on the bottom of a flow path in the substrate. When a dielectrophoresis (DEP) solution with a plurality of floating particles is conducted into the flow path and upon those electrodes and a voltage is applied to these electrodes, these particles would be driven by a negative DEP force to move upward to a specific depth in the flow path. Meanwhile, the optical tweezers of the apparatus is selectively focused at the specific depth in the flow path.