公开(公告)号：US20130072393A1

公开(公告)日：2013-03-21

申请号：US13518801

申请日：2009-12-24

申请人： Chenyu Zhang ,  Rui Liu ,  Cheng Wang ,  Yi Ba ,  Chunni Zhang ,  Ke Zeng

发明人： Chenyu Zhang ,  Rui Liu ,  Cheng Wang ,  Yi Ba ,  Chunni Zhang ,  Ke Zeng

IPC分类号： C12Q1/68 ,  C40B40/06

CPC分类号： C12Q1/6886 ,  C12Q1/6813 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/178 ,  C40B40/06

摘要： The present invention provides microRNAs for assessing the status of pancreatic cancer in a subject, and provides methods, kits, and biochips for detecting said microRNAs.

摘要翻译： 本发明提供了用于评估受试者中胰腺癌状态的微小RNA，并提供了用于检测所述微小RNA的方法，试剂盒和生物芯片。

公开(公告)号：US20100173288A1

公开(公告)日：2010-07-08

申请号：US12302196

申请日：2007-12-06

申请人： Chenyu Zhang ,  Junfeng Zhang ,  Xi Chen ,  Yi Ba ,  Jiangning Chen ,  Jin Wang ,  Ke Zeng ,  Hongjie Zhang

发明人： Chenyu Zhang ,  Junfeng Zhang ,  Xi Chen ,  Yi Ba ,  Jiangning Chen ,  Jin Wang ,  Ke Zeng ,  Hongjie Zhang

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6886 ,  C12Q1/6809 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/158 ,  C12Q2600/178 ,  C12Q2525/207

摘要： This invention provides a combination of microRNAs for evaluating the physiological and/or pathological condition of a subject, wherein the combination comprises all detectable microRNAs stably existing in the serum/plasma of a subject; and a method for evaluating the physiological and/or pathological condition of a subject, wherein the method includes determining all detectable microRNAs stably existing in the serum/plasma of a subject; and a kit for evaluating the physiological and/or pathological condition of a subject, wherein the kit contains the tools for determining all detectable microRNAs that stably existing in the serum/plasma of a subject; and a biochip for evaluating the physiological and/or pathological condition of a subject, wherein the biochip contains the components for determining all detectable microRNAs stably existing in the serum/plasma of a subject. The aforementioned combination, method, kit and biochip can be used for diagnosis as well as differentially diagnosis of diseases including various tumors; various acute/chronic infectious diseases, e.g. viral diseases such as viral influenza, viral hepatitis, AIDS, SARS, bacterial diseases such as tuberculosis, bacterial pneumonia, and other acute/chronic infectious diseases caused by various pathogenic microorganisms; other acute/chronic diseases such as diseases of respiratory system, diseases of immune system, diseases of blood and hematopoietic system, diseases of circulatory system such as cardio-cerebrovascular diseases, metabolic diseases of endocrine system, diseases of digestive system, diseases of nervous system, diseases of urinary system, diseases of reproductive system and diseases of locomotor system, prediction of complications occurrence and malignant diseases relapse, evaluation of therapeutic effects, screening of pharmaceutical active ingredients, assessment of drug efficacy as well as forensic authentication and prohibited drug inspection and the like, possessing a number of advantages such as extensive detection spectrum, high sensitivity, low cost, convenience for sampling, ease for sample preservation, etc. The said method can be widely used in work related to general survey of diseases and so on, improve the low-specificity and low-sensitivity caused by individual differences which single markers are difficult to overcome, significantly increasing the clinical detection rate of diseases, all of which make it become an effective means for diagnosing diseases in an early phase.

公开(公告)号：US09388470B2

公开(公告)日：2016-07-12

申请号：US12996967

申请日：2009-12-14

申请人： Chenyu Zhang ,  Ke Zeng ,  Junfeng Zhang ,  Yi Ba ,  Xi Chen ,  Haijin Li

发明人： Chenyu Zhang ,  Ke Zeng ,  Junfeng Zhang ,  Yi Ba ,  Xi Chen ,  Haijin Li

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

摘要： The present invention provides non-small cell lung cancer markers and the use thereof in diagnosing and monitoring diseases in vitro. The non-small cell lung cancer markers include at least one of the 26 selected detectable mature microRNAs existing stably in human serum or plasma. The invention also provides probe combinations, a kit and biochip for detecting the non-small cell lung cancer markers. The invention further provides a method for detecting the said lung cancer markers. The method in the present invention enables extensive detection spectrum, high sensitivity, low cost, convenient sample taking and preservation. The method can be applied in the general survey of disease, solves problems of the low specificity and sensitivity encountered with previous single markers, and increases significantly the clinical detection rate of diseases; it forms an effective means for diagnosing diseases at an early stage.

摘要翻译： 本发明提供非小细胞肺癌标志物及其在体外诊断和监测疾病中的应用。 非小细胞肺癌标志物包括稳定存在于人血清或血浆中的26种选择的可检测的成熟微RNA中的至少一种。 本发明还提供探针组合，用于检测非小细胞肺癌标志物的试剂盒和生物芯片。 本发明还提供了一种用于检测所述肺癌标志物的方法。 本发明的方法能够广泛的检测光谱，高灵敏度，低成本，便于取样和保存。 该方法可应用于疾病一般调查，解决了以前单个标记物遇到的低特异性和敏感性问题，大大提高了疾病的临床检出率; 它是早期诊断疾病的有效手段。

公开(公告)号：US20110117565A1

公开(公告)日：2011-05-19

申请号：US12996967

申请日：2009-12-14

申请人： Chenyu Zhang ,  Ke Zeng ,  Junfeng Zhang ,  Yi Ba ,  Xi Chen ,  Haijin Li

发明人： Chenyu Zhang ,  Ke Zeng ,  Junfeng Zhang ,  Yi Ba ,  Xi Chen ,  Haijin Li

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

摘要： The present invention provides non-small cell lung cancer markers and the use thereof. The non-small cell lung cancer markers in the present invention include at least one of the 26 selected detectable mature microRNAs existing stably in human serum or plasma. The invention also provides a probe combination, kit and biochip for detecting the non-small cell lung cancer markers. The invention further provides a method for detecting microRNAs in the serum of lung cancer patients. By detecting the variations of microRNAs in the serum of lung cancer patients, the disease can be diagnosed in vitro; the progression course of the disease can be predicted; the occurrence of complications, the rate of relapse and the prognosis of the disease can be monitored; the drug efficacy and therapeutic effects can be analyzed. The method in the present invention enables extensive detection spectrum, high sensitivity, low cost, convenient sample taking and preservation. The method can be applied in the general survey of disease, solves problems of the low specificity and sensitivity encountered with previous single markers, and increases significantly the clinical detection rate of diseases, all of which make it an effective means for diagnosing diseases at an early stage.

摘要翻译： 本发明提供非小细胞肺癌标志物及其用途。 本发明中的非小细胞肺癌标志物包括稳定存在于人血清或血浆中的26种选择的可检测的成熟微RNA中的至少一种。 本发明还提供了用于检测非小细胞肺癌标志物的探针组合，试剂盒和生物芯片。 本发明还提供了一种检测肺癌患者血清中微小RNA的方法。 通过检测肺癌患者血清中microRNAs的变化，可以在体外诊断该疾病; 可以预测疾病的进展过程; 可以监测并发症的发生，复发率和预后; 可以分析药物的疗效和治疗效果。 本发明的方法能够广泛的检测光谱，高灵敏度，低成本，便于取样和保存。 该方法可应用于疾病综合调查，解决了以前单个标记物所遇到的低特异性和敏感性问题，并大大增加了疾病的临床检出率，这些都成为早期诊断疾病的有效手段 阶段。