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    Methods and compositions for increasing longevity and protein yield from a cell culture
    3.
    发明申请
    Methods and compositions for increasing longevity and protein yield from a cell culture 审中-公开
    用于增加细胞培养物的寿命和蛋白质产量的方法和组合物

    公开(公告)号：US20070092947A1

    公开(公告)日：2007-04-26

    申请号：US11253666

    申请日：2005-10-20

    申请人： David Goldenberg ,  Zhengxing Qu ,  Eva Horak ,  Ivan Horak ,  Chien Chang ,  Edmund Rossi ,  Jeng-Dar Yang

    发明人： David Goldenberg ,  Zhengxing Qu ,  Eva Horak ,  Ivan Horak ,  Chien Chang ,  Edmund Rossi ,  Jeng-Dar Yang

    IPC分类号： C07H21/04 ,  C12P21/02 ,  C07K14/53 ,  C07K14/54 ,  C07K14/56 ,  C12N5/06 ,  C12N5/08

    CPC分类号： C12P21/02 ,  C12N2510/02

    摘要： Disclosed herein are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. By transfecting cells in culture with an apoptosis-inhibiting gene or vector, cells in culture can survive longer, resulting in extension of the state and yield of protein biosynthesis. Expression of the apoptosis-inhibitor within the cells, because it does not kill the cells, allows the cells, or an increased fraction thereof, to be maintained in culture for longer periods. This invention then allows for controlled, enhanced protein production of cell lines for commercial and research uses, particularly the enhanced production of growth factors, interferons, interleukins, hormones, enzymes, and monoclonal antibodies, and the like. The method preferentially involves eukaryotic cells in culture, and more advantageously mammalian cells in culture.

    摘要翻译： 本文公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，激素和疫苗。 通过用细胞凋亡抑制基因或载体转染培养细胞，培养中的细胞可以长时间存活，导致蛋白质生物合成的状态和产量的延长。 凋亡抑制剂在细胞内的表达，因为它不杀死细胞，允许细胞或其增加的部分在培养物中保持较长时间。 本发明然后允许用于商业和研究用途的细胞系的受控增强的蛋白质生产，特别是生长因子，干扰素，白细胞介素，激素，酶和单克隆抗体的增强生产等。 该方法优先涉及培养中的真核细胞，更有利于培养中的哺乳动物细胞。

    Methods and compositions for increasing longevity and protein yield from a cell culture
    4.
    发明授权
    Methods and compositions for increasing longevity and protein yield from a cell culture 有权
    用于增加细胞培养物的寿命和蛋白质产量的方法和组合物

    公开(公告)号：US07531327B2

    公开(公告)日：2009-05-12

    申请号：US11187863

    申请日：2005-07-25

    申请人： David M. Goldenberg ,  Zhengxing Qu ,  Eva Horak ,  Ivan D. Horak ,  Chien Hsing Chang ,  Edmund A. Rossi ,  Jeng-Dar Yang

    发明人： David M. Goldenberg ,  Zhengxing Qu ,  Eva Horak ,  Ivan D. Horak ,  Chien Hsing Chang ,  Edmund A. Rossi ,  Jeng-Dar Yang

    IPC分类号： C12N5/10 ,  C12N5/16

    CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K14/4747 ,  C07K16/18 ,  C07K16/2803 ,  C07K16/3007 ,  C07K2317/24 ,  C07K2317/31 ,  C12N5/0694 ,  C12N2500/90 ,  C12N2501/48 ,  C12N2510/02 ,  C12P21/02

    摘要： Disclosed herein are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. By transfecting cells in culture with an apoptosis-inhibiting gene or vector, cells in culture can survive longer, resulting in extension of the state and yield of protein biosynthesis. Expression of the apoptosis-inhibitor within the cells, because it does not kill the cells, allows the cells, or an increased fraction thereof, to be maintained in culture for longer periods. This invention then allows for controlled, enhanced protein production of cell lines for commercial and research uses, particularly the enhanced production of growth factors, interferons, interleukins, hormones, enzymes, and monoclonal antibodies, and the like. The method preferentially involves eukaryotic cells in culture, and more advantageously mammalian cells in culture.

    摘要翻译： 本文公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，激素和疫苗。 通过用细胞凋亡抑制基因或载体转染培养细胞，培养中的细胞可以长时间存活，导致蛋白质生物合成的状态和产量的延长。 凋亡抑制剂在细胞内的表达，因为它不杀死细胞，允许细胞或其增加的部分在培养物中保持较长时间。 本发明然后允许用于商业和研究用途的细胞系的受控增强的蛋白质生产，特别是生长因子，干扰素，白细胞介素，激素，酶和单克隆抗体的增强生产等。 该方法优先涉及培养中的真核细胞，更有利于培养中的哺乳动物细胞。