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    Methods and compositions for increasing longevity and protein yield from a cell culture
    2.
    发明申请
    Methods and compositions for increasing longevity and protein yield from a cell culture 审中-公开
    用于增加细胞培养物的寿命和蛋白质产量的方法和组合物

    公开(公告)号：US20070092947A1

    公开(公告)日：2007-04-26

    申请号：US11253666

    申请日：2005-10-20

    申请人： David Goldenberg ,  Zhengxing Qu ,  Eva Horak ,  Ivan Horak ,  Chien Chang ,  Edmund Rossi ,  Jeng-Dar Yang

    发明人： David Goldenberg ,  Zhengxing Qu ,  Eva Horak ,  Ivan Horak ,  Chien Chang ,  Edmund Rossi ,  Jeng-Dar Yang

    IPC分类号： C07H21/04 ,  C12P21/02 ,  C07K14/53 ,  C07K14/54 ,  C07K14/56 ,  C12N5/06 ,  C12N5/08

    CPC分类号： C12P21/02 ,  C12N2510/02

    摘要： Disclosed herein are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. By transfecting cells in culture with an apoptosis-inhibiting gene or vector, cells in culture can survive longer, resulting in extension of the state and yield of protein biosynthesis. Expression of the apoptosis-inhibitor within the cells, because it does not kill the cells, allows the cells, or an increased fraction thereof, to be maintained in culture for longer periods. This invention then allows for controlled, enhanced protein production of cell lines for commercial and research uses, particularly the enhanced production of growth factors, interferons, interleukins, hormones, enzymes, and monoclonal antibodies, and the like. The method preferentially involves eukaryotic cells in culture, and more advantageously mammalian cells in culture.

    摘要翻译： 本文公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，激素和疫苗。 通过用细胞凋亡抑制基因或载体转染培养细胞，培养中的细胞可以长时间存活，导致蛋白质生物合成的状态和产量的延长。 凋亡抑制剂在细胞内的表达，因为它不杀死细胞，允许细胞或其增加的部分在培养物中保持较长时间。 本发明然后允许用于商业和研究用途的细胞系的受控增强的蛋白质生产，特别是生长因子，干扰素，白细胞介素，激素，酶和单克隆抗体的增强生产等。 该方法优先涉及培养中的真核细胞，更有利于培养中的哺乳动物细胞。

    RS7 ANTIBODIES
    8.
    发明申请
    RS7 ANTIBODIES 有权
    RS7抗体

    公开(公告)号：US20070212350A1

    公开(公告)日：2007-09-13

    申请号：US11745896

    申请日：2007-05-08

    申请人： Serengulam Govindan ,  Zhengxing Qu ,  Hans Hansen ,  David Goldenberg

    发明人： Serengulam Govindan ,  Zhengxing Qu ,  Hans Hansen ,  David Goldenberg

    IPC分类号： A61K39/44 ,  A61K39/395 ,  C07K16/30 ,  C12P21/08

    CPC分类号： A61K45/06 ,  A61K39/39558 ,  A61K51/1045 ,  A61K51/1051 ,  A61K2039/505 ,  C07K16/30 ,  C07K16/3015 ,  C07K2317/21 ,  C07K2317/24

    摘要： This invention relates to monovalent and multivalent, monospecific binding proteins and to multivalent, multispecific binding proteins. One embodiment of these binding proteins has one or more binding sites where each binding site binds with a target antigen or an epitope on a target antigen. Another embodiment of these binding proteins has two or more binding sites where each binding site has affinity towards different epitopes on a target antigen or has affinity towards either a target antigen or a hapten. The present invention further relates to recombinant vectors useful for the expression of these functional binding proteins in a host. More specifically, the present invention relates to the tumor-associated antigen binding protein designated RS7, and other EGP-1 binding-proteins. The invention further relates to humanized, human and chimeric RS7 antigen binding proteins, and the use of such binding proteins in diagnosis and therapy.

    摘要翻译： 本发明涉及单价和多价单特异性结合蛋白和多价多特异性结合蛋白。 这些结合蛋白的一个实施方案具有一个或多个结合位点，其中每个结合位点与靶抗原或靶抗原上的表位结合。 这些结合蛋白的另一个实施方案具有两个或更多个结合位点，其中每个结合位点对靶抗原上的不同表位具有亲和力，或对靶抗原或半抗原具有亲和力。 本发明还涉及可用于在宿主中表达这些功能性结合蛋白的重组载体。 更具体地，本发明涉及称为RS7的肿瘤相关抗原结合蛋白和其它EGP-1结合蛋白。 本发明还涉及人源化，人和嵌合RS7抗原结合蛋白，以及这种结合蛋白在诊断和治疗中的用途。

    RS7 ANTIBODIES
    9.
    发明申请
    RS7 ANTIBODIES 有权
    RS7抗体

    公开(公告)号：US20080131363A1

    公开(公告)日：2008-06-05

    申请号：US11868290

    申请日：2007-10-05

    申请人： Serengulam Govindan ,  Zhengxing Qu ,  Hans Hansen ,  David Goldenberg

    发明人： Serengulam Govindan ,  Zhengxing Qu ,  Hans Hansen ,  David Goldenberg

    IPC分类号： A61K39/395 ,  A61K38/20 ,  A61K51/10 ,  A61P35/04

    CPC分类号： C07K16/30 ,  A61K38/164 ,  A61K38/168 ,  A61K38/1816 ,  A61K38/19 ,  A61K38/21 ,  A61K45/06 ,  A61K47/6805 ,  A61K47/6857 ,  A61K51/1054 ,  A61K2039/505 ,  C07K16/28 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/94 ,  A61K2300/00

    摘要： This invention relates to monovalent and multivalent, monospecific binding proteins and to multivalent, multispecific binding proteins. One embodiment of these binding proteins has one or more binding sites where each binding site binds with a target antigen or an epitope on a target antigen. Another embodiment of these binding proteins has two or more binding sites where each binding site has affinity towards different epitopes on a target antigen or has affinity towards either a target antigen or a hapten. The present invention further relates to recombinant vectors useful for the expression of these functional binding proteins in a host. More specifically, the present invention relates to the tumor-associated antigen binding protein designated RS7, and other EGP-1 binding-proteins. The invention further relates to humanized, human and chimeric RS7 antigen binding proteins, and the use of such binding proteins in diagnosis and therapy.

    摘要翻译： 本发明涉及单价和多价单特异性结合蛋白和多价多特异性结合蛋白。 这些结合蛋白的一个实施方案具有一个或多个结合位点，其中每个结合位点与靶抗原或靶抗原上的表位结合。 这些结合蛋白的另一个实施方案具有两个或更多个结合位点，其中每个结合位点对靶抗原上的不同表位具有亲和力，或对靶抗原或半抗原具有亲和力。 本发明还涉及可用于在宿主中表达这些功能性结合蛋白的重组载体。 更具体地，本发明涉及称为RS7的肿瘤相关抗原结合蛋白和其它EGP-1结合蛋白。 本发明还涉及人源化，人和嵌合RS7抗原结合蛋白，以及这种结合蛋白在诊断和治疗中的用途。