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公开(公告)号：US20130017960A1
公开(公告)日：2013-01-17
申请号：US13551486
申请日：2012-07-17
CPC分类号： C12Q1/6823 , C12Q1/6809 , C12Q1/6853 , C12Q1/6858 , C12Q2563/167 , C12Q2565/518 , C12Q2537/143
摘要： Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.
摘要翻译： 本文提供了用于检测多个靶核酸的存在或不存在的产物和方法。 某些方法包括扩增靶核酸或其部分; 扩增与扩增子杂交的寡核苷酸，其中延伸的寡核苷酸包括捕获剂; 通过捕获剂将延伸的寡核苷酸捕获到固相; 通过与竞争对手的竞争释放延伸的寡核苷酸; 检测延伸的寡核苷酸，从而通过存在或不存在延伸的寡核苷酸来确定每种靶核酸的存在或不存在。
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公开(公告)号：US10604791B2
公开(公告)日：2020-03-31
申请号：US13551486
申请日：2012-07-17
IPC分类号： C12Q1/68 , C12P19/34 , C12Q1/6823 , C12Q1/6809 , C12Q1/6853 , C12Q1/6858
摘要： Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.
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