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公开(公告)号：US20100216181A1
公开(公告)日：2010-08-26
申请号：US12709764
申请日：2010-02-22
IPC分类号： C12Q1/02 , C12N5/0789
CPC分类号： C12N5/0606 , C12N5/0647 , C12N5/069 , C12N2500/02 , C12N2500/90 , C12N2501/115 , C12N2501/155 , C12N2501/165 , C12N2501/70 , C12N2506/02
摘要： The present invention provides methods and compositions for the production of hematopoietic progenitor cells or endothelial progenitor cells from human pluripotent stem cells using a defined cell culture medium without the need to utilize feeder cells or serum. In some embodiments, differentiation is accomplished using hypoxic atmospheric conditions. The defined medium of the present invention may contain growth factors and a matrix component. The hematopoietic progenitor cells may be further differentiated into cell lineages including red blood cells, macrophages, granulocytes, and megakaryocytes. The endothelial progenitor cells may be further differentiated into endothelial cells. Also disclosed are screening assays for identification of candidate substances that affect differentiation of pluripotent stem cells into progenitor cells.
摘要翻译： 本发明提供了使用限定的细胞培养基从人多能干细胞生产造血祖细胞或内皮祖细胞而不需要利用饲养细胞或血清的方法和组合物。 在一些实施方案中，使用缺氧大气条件实现分化。 本发明的限定的培养基可以含有生长因子和基质成分。 造血祖细胞可进一步分化成细胞系，包括红细胞，巨噬细胞，粒细胞和巨核细胞。 内皮祖细胞可进一步分化为内皮细胞。 还公开了用于鉴定影响多能干细胞分化成祖细胞的候选物质的筛选试验。
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公开(公告)号：US08557580B2
公开(公告)日：2013-10-15
申请号：US12709764
申请日：2010-02-22
CPC分类号： C12N5/0606 , C12N5/0647 , C12N5/069 , C12N2500/02 , C12N2500/90 , C12N2501/115 , C12N2501/155 , C12N2501/165 , C12N2501/70 , C12N2506/02
摘要： The present invention provides methods and compositions for the production of hematopoietic progenitor cells or endothelial progenitor cells from human pluripotent stem cells using a defined cell culture medium without the need to utilize feeder cells or serum. In some embodiments, differentiation is accomplished using hypoxic atmospheric conditions. The defined medium of the present invention may contain growth factors and a matrix component. The hematopoietic progenitor cells may be further differentiated into cell lineages including red blood cells, macrophages, granulocytes, and megakaryocytes. The endothelial progenitor cells may be further differentiated into endothelial cells. Also disclosed are screening assays for identification of candidate substances that affect differentiation of pluripotent stem cells into progenitor cells.
摘要翻译： 本发明提供了使用限定的细胞培养基从人多能干细胞生产造血祖细胞或内皮祖细胞而不需要利用饲养细胞或血清的方法和组合物。 在一些实施方案中，使用缺氧大气条件实现分化。 本发明的限定的培养基可以含有生长因子和基质成分。 造血祖细胞可进一步分化成细胞系，包括红细胞，巨噬细胞，粒细胞和巨核细胞。 内皮祖细胞可进一步分化为内皮细胞。 还公开了用于鉴定影响多能干细胞分化成祖细胞的候选物质的筛选试验。
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公开(公告)号：US08815585B2
公开(公告)日：2014-08-26
申请号：US12164969
申请日：2008-06-30
CPC分类号： C12N5/0606 , C12M23/50 , C12M33/06 , C12M33/07 , C12M41/48 , C12N2501/70 , C12N2501/999 , C12N2509/00
摘要： The invention concerns methods for automated culture of embryonic stem cells (ESCs) such as human ESCs. In some aspects, methods of the invention employ optimized culture media and limited proteolytic treatment of cells to separate cell clusters for expansion. Automated systems for passage and expansion of ESCs are also provided.
摘要翻译： 本发明涉及用于胚胎干细胞（ESC）如人ESC的自动培养的方法。 在一些方面，本发明的方法使用优化的培养基和有限的蛋白水解处理细胞以分离细胞簇以进行扩增。 还提供了ESCs通过和扩建的自动化系统。
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公开(公告)号：US08513012B2
公开(公告)日：2013-08-20
申请号：US12435123
申请日：2009-05-04
申请人： Christine Daigh , Deepika Rajesh
发明人： Christine Daigh , Deepika Rajesh
CPC分类号： C12N5/0642 , C12N2501/125 , C12N2501/145 , C12N2501/155 , C12N2501/165 , C12N2501/22 , C12N2501/23 , C12N2501/26 , C12N2502/1394 , C12N2506/02 , C12N2509/00
摘要： Provided are methods for generating mast cells from pluripotent stem cells in vitro. Methods are disclosed for the differentiation of pluripotent cells, such as iPS cells and/or human embryonic stem cells, into mast cells. The resulting mast cells may be used for various purposes including screening cells for drug development and research. Growth factors which may be included in culture media according to the present invention include stem cell factor (SCF), FLT-3 ligand, thrombopoietin (TPO), interleukin-3 (IL-3), and/or interleukin-6 (IL-6).
摘要翻译： 提供了在体外从多能干细胞产生肥大细胞的方法。 公开了将多能细胞如iPS细胞和/或人胚胎干细胞分化为肥大细胞的方法。 所得的肥大细胞可用于各种目的，包括用于药物开发和研究的筛选细胞。 根据本发明可以包括在培养基中的生长因子包括干细胞因子（SCF），FLT-3配体，血小板生成素（TPO），白细胞介素-3（IL-3）和/或白细胞介素-6（IL- 6）。
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公开(公告)号：US20090029462A1
公开(公告)日：2009-01-29
申请号：US12164969
申请日：2008-06-30
CPC分类号： C12N5/0606 , C12M23/50 , C12M33/06 , C12M33/07 , C12M41/48 , C12N2501/70 , C12N2501/999 , C12N2509/00
摘要： The invention concerns methods for automated culture of embryonic stem cells (ESCs) such as human ESCs. In some aspects, methods of the invention employ optimized culture media and limited proteolytic treatment of cells to separate cell clusters for expansion. Automated systems for passage and expansion of ESCs are also provided.
摘要翻译： 本发明涉及用于胚胎干细胞（ESC）如人ESC的自动培养的方法。 在一些方面，本发明的方法使用优化的培养基和有限的蛋白水解处理细胞以分离细胞簇以进行扩增。 还提供了ESCs通过和扩建的自动化系统。
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公开(公告)号：US20100291603A1
公开(公告)日：2010-11-18
申请号：US12532453
申请日：2008-03-28
申请人： Whyte G. Owen , Dong Chen , Christine Daigh
发明人： Whyte G. Owen , Dong Chen , Christine Daigh
CPC分类号： G01N33/86 , G01N2333/755
摘要： This document relates to methods and materials involved in assessing blood components (e.g., assessing von Willebrand factor activity or platelet activity) in mammals. For example, methods and materials involved in using labeled platelets to assess von Willebrand factor activity in a sample (e.g., plasma) from a mammal (e.g., a human) are provided.
摘要翻译： 本文件涉及在哺乳动物中评估血液成分（例如评估血管性血友病因子活性或血小板活性）所涉及的方法和材料。 例如，提供了使用标记血小板来评价来自哺乳动物（例如人）的样品（例如血浆）中的血管性血友病因子活性的方法和材料。
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公开(公告)号：US20090275131A1
公开(公告)日：2009-11-05
申请号：US12435123
申请日：2009-05-04
申请人： Christine Daigh , Deepika Rajesh
发明人： Christine Daigh , Deepika Rajesh
IPC分类号： C12N5/08
CPC分类号： C12N5/0642 , C12N2501/125 , C12N2501/145 , C12N2501/155 , C12N2501/165 , C12N2501/22 , C12N2501/23 , C12N2501/26 , C12N2502/1394 , C12N2506/02 , C12N2509/00
摘要： Provided are methods for generating mast cells from pluripotent stem cells in vitro. Methods are disclosed for the differentiation of pluripotent cells, such as iPS cells and/or human embryonic stem cells, into mast cells. The resulting mast cells may be used for various purposes including screening cells for drug development and research. Growth factors which may be included in culture media according to the present invention include stem cell factor (SCF), FLT-3 ligand, thrombopoietin (TPO), interleukin-3 (IL-3), and/or interleukin-6 (IL-6).
摘要翻译： 提供了在体外从多能干细胞产生肥大细胞的方法。 公开了将多能细胞如iPS细胞和/或人胚胎干细胞分化为肥大细胞的方法。 所得的肥大细胞可用于各种目的，包括用于药物开发和研究的筛选细胞。 根据本发明可以包括在培养基中的生长因子包括干细胞因子（SCF），FLT-3配体，血小板生成素（TPO），白细胞介素-3（IL-3）和/或白细胞介素-6（IL- 6）。
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