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公开(公告)号：US09017557B2
公开(公告)日：2015-04-28
申请号：US11658481
申请日：2005-07-27
IPC分类号： C07H21/02 , G01N33/483 , B01J20/28 , B01J20/283 , G01N30/52
CPC分类号： B01J20/28042 , B01J20/283 , B01J2220/82 , G01N2030/528
摘要： The present invention concerns new monolithic shaped bodies, a method for their preparation as well as their use especially for the selective purification and separation of biopolymers. A chromatographic separation material is provided with the new monolithic shaped bodies that allows a selective, efficient and reproducible purification and separation of biopolymers.
摘要翻译： 本发明涉及新的整体成形体，其制备方法以及它们特别用于选择性纯化和分离生物聚合物的用途。 提供了一种色谱分离材料，其具有允许生物聚合物的选择性，有效和可再现的纯化和分离的新的整体形状体。
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公开(公告)号：US20090062522A1
公开(公告)日：2009-03-05
申请号：US11658481
申请日：2005-07-27
IPC分类号： C07H21/02 , G01N33/483
CPC分类号： B01J20/28042 , B01J20/283 , B01J2220/82 , G01N2030/528
摘要： The present invention concerns new monolithic shaped bodies, a method for their preparation as well as their use especially for the selective purification and separation of biopolymers. A chromatographic separation material is provided with the new monolithic shaped bodies that allows a selective, efficient and reproducible purification and separation of biopolymers.
摘要翻译： 本发明涉及新的整体成形体，其制备方法以及它们特别用于选择性纯化和分离生物聚合物的用途。 提供了一种色谱分离材料，其具有允许生物聚合物的选择性，有效和可再现的纯化和分离的新的整体形状体。
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公开(公告)号：US08460941B2
公开(公告)日：2013-06-11
申请号：US13465474
申请日：2012-05-07
IPC分类号： G01N33/00
CPC分类号： C12N15/101 , C12N15/1006 , Y10T436/143333 , Y10T436/255
摘要： The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention.
摘要翻译： 本发明涉及从包含所述靶核酸的样品中分离靶核酸的方法，包括以下步骤：将含有所述靶核酸的样品与结合溶液和核酸结合基质混合，将至少部分 的所述靶核酸与所述核酸结合基质反应，其中所述核酸结合基质同时被处理或已经用至少一种包含选自主要组13至16的金属的金属的化合物进行处理， 半金属和过渡金属，用于降低非靶核酸污染物，或其中所述核酸结合基质用疏水基团修饰。 此外，本发明的教导提供了相应的试剂盒和试剂。
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公开(公告)号：US08206990B2
公开(公告)日：2012-06-26
申请号：US12439396
申请日：2007-10-10
IPC分类号： G01N30/56
CPC分类号： C12N15/101 , C12N15/1006 , Y10T436/143333 , Y10T436/255
摘要： The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention.
摘要翻译： 本发明涉及从包含所述靶核酸的样品中分离靶核酸的方法，包括以下步骤：将含有所述靶核酸的样品与结合溶液和核酸结合基质混合，将至少部分 的所述靶核酸与所述核酸结合基质反应，其中所述核酸结合基质同时被处理或已经用至少一种包含选自主要组13至16的金属的金属的化合物进行处理， 半金属和过渡金属，用于降低非靶核酸污染物，或其中所述核酸结合基质用疏水基团修饰。 此外，本发明的教导提供了相应的试剂盒和试剂。
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公开(公告)号：US20100056769A1
公开(公告)日：2010-03-04
申请号：US12439396
申请日：2007-10-10
CPC分类号： C12N15/101 , C12N15/1006 , Y10T436/143333 , Y10T436/255
摘要： The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention.
摘要翻译： 本发明涉及从包含所述靶核酸的样品中分离靶核酸的方法，包括以下步骤：将含有所述靶核酸的样品与结合溶液和核酸结合基质混合，将至少部分 的所述靶核酸与所述核酸结合基质反应，其中所述核酸结合基质同时被处理或已经用至少一种包含选自主要组13至16的金属的金属的化合物进行处理， 半金属和过渡金属，用于降低非靶核酸污染物，或其中所述核酸结合基质用疏水基团修饰。 此外，本发明的教导提供了相应的试剂盒和试剂。
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公开(公告)号：US09790250B2
公开(公告)日：2017-10-17
申请号：US12995341
申请日：2009-05-12
CPC分类号： C07H1/08 , C12N15/1006
摘要： The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterized by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧25% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood.
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公开(公告)号：US07183002B2
公开(公告)日：2007-02-27
申请号：US10239112
申请日：2001-03-22
申请人： Philippe Sauer , Bernd Springer , Thomas Manz , Christoph Ritt , Roland Fabis
发明人： Philippe Sauer , Bernd Springer , Thomas Manz , Christoph Ritt , Roland Fabis
CPC分类号： B03C1/01 , B82Y25/00 , C12N15/1013 , H01F1/0054 , Y10T428/2982 , Y10T428/2993 , Y10T428/2996 , Y10T428/2998
摘要： Porous, ferro- or ferrimagnetic, glass particles are described that selectively bind molecules of interest, especially nucleic acid molecules, under appropriate conditions. Methods of preparing the porous, ferro- or ferrimagnetic, glass particles and their use of identifying or separating molecules of interest are also described. Kits comprising the porous, ferro- or ferrimagnetic, glass particles are also provided.
摘要翻译： 描述了多孔，铁或铁磁性玻璃颗粒，其在合适的条件下选择性地结合感兴趣的分子，特别是核酸分子。 还描述了制备多孔，铁或铁磁性玻璃颗粒的方法及其鉴定或分离感兴趣的分子的用途。 还提供了包含多孔，铁或铁磁性玻璃颗粒的试剂盒。
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公开(公告)号：US07977109B2
公开(公告)日：2011-07-12
申请号：US12096155
申请日：2006-12-08
申请人： Christoph Ritt , Ralf Himmelreich , Martin Weber
发明人： Christoph Ritt , Ralf Himmelreich , Martin Weber
IPC分类号： G01N30/00
CPC分类号： C12N15/101 , Y10T436/143333 , Y10T436/255
摘要： The present invention relates to a method for enriching nucleic acids with a length of not more than 300 nucleotides. The invention also relates to a kit for enriching nucleic acids with a length of not more than 300 nucleotides, to the use of such a kit, to the use of an anion exchange matrix and to a method for treating a disease.
摘要翻译： 本发明涉及一种富集长度不超过300个核苷酸的核酸的方法。 本发明还涉及用于富集长度不超过300个核苷酸的核酸的试剂盒，使用这种试剂盒，使用阴离子交换基质和治疗疾病的方法。
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公开(公告)号：US07922917B2
公开(公告)日：2011-04-12
申请号：US11635304
申请日：2006-12-07
申请人： Philippe Sauer , Bernd Springer , Thomas Manz , Christoph Ritt , Roland Fabis
发明人： Philippe Sauer , Bernd Springer , Thomas Manz , Christoph Ritt , Roland Fabis
IPC分类号： C01G49/02
CPC分类号： B03C1/01 , B82Y25/00 , C12N15/1013 , H01F1/0054 , Y10T428/2982 , Y10T428/2993 , Y10T428/2996 , Y10T428/2998
摘要： Porous, ferro- or ferrimagnetic, glass particles are described that selectively bind molecules of interest, especially nucleic acid molecules, under appropriate conditions. Methods of preparing the porous, ferro- or ferrimagnetic, glass particles and their use for identifying or separating molecules of interest are also described. Kits comprising the porous, ferro- or ferrimagnetic, glass particles are also provided.
摘要翻译： 描述了多孔，铁或铁磁性玻璃颗粒，其在合适的条件下选择性地结合感兴趣的分子，特别是核酸分子。 还描述了制备多孔，铁或铁磁性玻璃颗粒的方法及其用于鉴定或分离目的分子的用途。 还提供了包含多孔，铁或铁磁性玻璃颗粒的试剂盒。
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公开(公告)号：US20110160446A1
公开(公告)日：2011-06-30
申请号：US12995341
申请日：2009-05-12
IPC分类号： C07H1/06
CPC分类号： C07H1/08 , C12N15/1006
摘要： The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧25% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood.
摘要翻译： 本发明涉及用于从含有起始物质的核酸分离和/或纯化核酸，特别是短链核酸的方法和试剂盒，其特征在于以下方法步骤：（a）将核酸结合至 核酸结合载体材料，其中原料在至少一种离液序列化合物和优选异丙醇的存在下与核酸键合载体材料接触，其中异丙醇以≥25％（v / v）的浓度存在 ）和＆lt; nlE; 35％（v / v），（b）从核酸粘合载体材料任选洗脱键合的核酸。 所述方法特别适用于从母体血液中纯化胎儿DNA。
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