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公开(公告)号：US06297037B1
公开(公告)日：2001-10-02
申请号：US08194664
申请日：1994-02-10
IPC分类号： C12N928
CPC分类号： C12N9/2417 , C11D3/386 , C12N15/75 , C12P19/14
摘要： Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
摘要翻译： 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。
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公开(公告)号：US5824532A
公开(公告)日：1998-10-20
申请号：US468220
申请日：1995-06-06
IPC分类号： C12N15/09 , C11D3/386 , C12N1/21 , C12N5/10 , C12N9/26 , C12N9/28 , C12N15/56 , C12N15/75 , C12P19/14 , C12N15/63
CPC分类号： C12N9/2417 , C11D3/386 , C12N15/75 , C12P19/14
摘要： Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
摘要翻译： 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。
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公开(公告)号：US5849549A
公开(公告)日：1998-12-15
申请号：US468698
申请日：1995-06-06
申请人： Christopher C. Barnett , Leif P. Solheim , Colin Mitchinson , Scott D. Power , Carol A. Requadt
发明人： Christopher C. Barnett , Leif P. Solheim , Colin Mitchinson , Scott D. Power , Carol A. Requadt
IPC分类号： C12N15/09 , C11D3/386 , C12N1/21 , C12N5/10 , C12N9/26 , C12N9/28 , C12N15/56 , C12N15/75 , C12P19/14
CPC分类号： C12N9/2417 , C11D3/386 , C12N15/75 , C12P19/14
摘要： Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
摘要翻译： 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。
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4.发明申请公开(公告)号：US20100003366A1
公开(公告)日：2010-01-07
申请号：US12479416
申请日：2009-06-05
申请人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David E. Estell , Louise Wallace , Regina Chin , Carol A. Requadt , Scott D. Power , Michael J. Pepsin
发明人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David E. Estell , Louise Wallace , Regina Chin , Carol A. Requadt , Scott D. Power , Michael J. Pepsin
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
摘要： Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
摘要翻译： 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。
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5.发明授权Production of glucose from starch using alpha-amylases from Bacillus subtilis 有权使用来自枯草芽孢杆菌的α-淀粉酶从淀粉生产葡萄糖公开(公告)号：US09040278B2
公开(公告)日：2015-05-26
申请号：US12478266
申请日：2009-06-04
申请人： Luis G. Cascao-Pereira , Regina Chin , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Michael J. Pepsin , Scott D. Power , Sandra W. Ramer , Carol A. Requadt , Andrew Shaw , Amr R. Toppozada , Louise Wallace
发明人： Luis G. Cascao-Pereira , Regina Chin , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Michael J. Pepsin , Scott D. Power , Sandra W. Ramer , Carol A. Requadt , Andrew Shaw , Amr R. Toppozada , Louise Wallace
IPC分类号： C12N9/28 , C12P19/14 , C12P7/06 , C08B37/00 , C07H21/04 , C12P19/02 , C11D3/22 , D06L1/14 , C11D3/386
摘要： An α-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
摘要翻译： 来自枯草芽孢杆菌（AmyE）的α-淀粉酶从各种碳水化合物底物（包括植物淀粉，麦芽庚糖和麦芽三糖）产生显着量的葡萄糖。 除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。 葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。
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