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1.发明授权Production of glucose from starch using alpha-amylases from Bacillus subtilis 有权使用来自枯草芽孢杆菌的α-淀粉酶从淀粉生产葡萄糖公开(公告)号：US09040278B2
公开(公告)日：2015-05-26
申请号：US12478266
申请日：2009-06-04
申请人： Luis G. Cascao-Pereira , Regina Chin , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Michael J. Pepsin , Scott D. Power , Sandra W. Ramer , Carol A. Requadt , Andrew Shaw , Amr R. Toppozada , Louise Wallace
发明人： Luis G. Cascao-Pereira , Regina Chin , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Michael J. Pepsin , Scott D. Power , Sandra W. Ramer , Carol A. Requadt , Andrew Shaw , Amr R. Toppozada , Louise Wallace
IPC分类号： C12N9/28 , C12P19/14 , C12P7/06 , C08B37/00 , C07H21/04 , C12P19/02 , C11D3/22 , D06L1/14 , C11D3/386
摘要： An α-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
摘要翻译： 来自枯草芽孢杆菌（AmyE）的α-淀粉酶从各种碳水化合物底物（包括植物淀粉，麦芽庚糖和麦芽三糖）产生显着量的葡萄糖。 除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。 葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。
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2.发明申请PRODUCTION OF GLUCOSE FROM STARCH USING ALPHA-AMYLASES FROM BACILLUS SUBTILIS 有权使用来自BACILLUS SUBTILIS的ALPHA-AMYLASES从淀粉生产葡萄糖公开(公告)号：US20090305935A1
公开(公告)日：2009-12-10
申请号：US12478266
申请日：2009-06-04
申请人： Luis G. CASCAO-PEREIRA , Regina CHIN , William A. CUEVAS , David A. ESTELL , Sang-Kyu LEE , Michael J. PEPSIN , Scott D. POWER , Sandra W. RAMER , Carol A. REQUADT , Andrew SHAW , Amr R. TOPPOZADA , Louise WALLACE
发明人： Luis G. CASCAO-PEREIRA , Regina CHIN , William A. CUEVAS , David A. ESTELL , Sang-Kyu LEE , Michael J. PEPSIN , Scott D. POWER , Sandra W. RAMER , Carol A. REQUADT , Andrew SHAW , Amr R. TOPPOZADA , Louise WALLACE
摘要： An α-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
摘要翻译： 来自枯草芽孢杆菌（AmyE）的α-淀粉酶从各种碳水化合物底物（包括植物淀粉，麦芽七糖和麦芽三糖）产生显着量的葡萄糖。 除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。 葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。
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3.发明申请PSEUDOMONAS SACCHAROPHILA G4-AMYLASE VARIANTS AND USES THEREOF 审中-公开PSEUDOMONAS SACCHAROPHILA G4-氨基酸变体及其用途公开(公告)号：US20110033575A1
公开(公告)日：2011-02-10
申请号：US12829696
申请日：2010-07-02
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/35 , Y02E50/16 , Y02E50/17
摘要： Pseudomonas saccharophila G4-forming amylase (PS4) variants, and nucleic acids encoding these, and their uses in producing food products and in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch are provided.
摘要翻译： 提供了糖酵母（Saccharophila）G4形成淀粉酶（PS4）变体，以及编码它们的核酸，以及它们在食品生产中的用途和在酶催化的高温液化步骤中从淀粉例如玉米淀粉生产乙醇的用途。
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4.发明申请公开(公告)号：US20100003366A1
公开(公告)日：2010-01-07
申请号：US12479416
申请日：2009-06-05
申请人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David E. Estell , Louise Wallace , Regina Chin , Carol A. Requadt , Scott D. Power , Michael J. Pepsin
发明人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David E. Estell , Louise Wallace , Regina Chin , Carol A. Requadt , Scott D. Power , Michael J. Pepsin
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
摘要： Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
摘要翻译： 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。
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5.发明申请Process of obtaining ethanol without glucoamylase using pseudomonas saccharophila G4-amylase and variants thereof 失效使用假单胞菌G4-淀粉酶及其变体获得没有葡糖淀粉酶的乙醇的方法公开(公告)号：US20090311764A1
公开(公告)日：2009-12-17
申请号：US12318513
申请日：2008-12-30
申请人： Andrew Shaw , Regina Chin , Karsten M. Kragh
发明人： Andrew Shaw , Regina Chin , Karsten M. Kragh
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/35 , Y02E50/16 , Y02E50/17
摘要： Pseudomonas saccharophila G4-forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.
摘要翻译： 糖酵母（Saccharophila）G4形成淀粉酶（PS4）及其变体有利地可用于酶催化的高温液化步骤以从淀粉例如玉米淀粉生产乙醇。 PS4产生显着量的麦芽三糖，其可以在随后的发酵步骤中被酿酒酵母用于产生乙醇。 PS4的这种性质有利地允许在没有糖化步骤的情况下从液化淀粉生产乙醇。 提供了PS4变体，其具有改进的性质，例如热稳定性和/或改变的外切特异性和内切特异性淀粉酶活性。
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6.发明授权公开(公告)号：US09090887B2
公开(公告)日：2015-07-28
申请号：US12479416
申请日：2009-06-05
申请人： William A Cuevas , Sang-Kyu Lee , Sandra W Ramer , Andrew Shaw , Amr R Toppozada , David E Estell , Louise Wallace , Regina Chin , Carol A Requadt , Scott D Power , Michael J Pepsin
发明人： William A Cuevas , Sang-Kyu Lee , Sandra W Ramer , Andrew Shaw , Amr R Toppozada , David E Estell , Louise Wallace , Regina Chin , Carol A Requadt , Scott D Power , Michael J Pepsin
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
摘要： Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
摘要翻译： 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。
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7.发明授权Process of obtaining ethanol without glucoamylase using Pseudomonas saccharophila G4-amylase variants thereof 失效使用酿酒酵母G4-淀粉酶变体获得没有葡糖淀粉酶的乙醇的方法公开(公告)号：US08318451B2
公开(公告)日：2012-11-27
申请号：US12318513
申请日：2008-12-30
申请人： Andrew Shaw , Regina Chin , Karsten M. Kragh
发明人： Andrew Shaw , Regina Chin , Karsten M. Kragh
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/35 , Y02E50/16 , Y02E50/17
摘要： Pseudomonas saccharophila G4-forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.
摘要翻译： 糖酵母（Saccharophila）G4形成淀粉酶（PS4）及其变体有利地可用于酶催化的高温液化步骤以从淀粉例如玉米淀粉生产乙醇。 PS4产生显着量的麦芽三糖，其可以在随后的发酵步骤中被酿酒酵母用于产生乙醇。 PS4的这种性质有利地允许在没有糖化步骤的情况下从液化淀粉生产乙醇。 提供了PS4变体，其具有改进的性质，例如热稳定性和/或改变的外切特异性和内切特异性淀粉酶活性。
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