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公开(公告)号：US5849549A
公开(公告)日：1998-12-15
申请号：US468698
申请日：1995-06-06
申请人： Christopher C. Barnett , Leif P. Solheim , Colin Mitchinson , Scott D. Power , Carol A. Requadt
发明人： Christopher C. Barnett , Leif P. Solheim , Colin Mitchinson , Scott D. Power , Carol A. Requadt
IPC分类号： C12N15/09 , C11D3/386 , C12N1/21 , C12N5/10 , C12N9/26 , C12N9/28 , C12N15/56 , C12N15/75 , C12P19/14
CPC分类号： C12N9/2417 , C11D3/386 , C12N15/75 , C12P19/14
摘要： Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
摘要翻译： 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。
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公开(公告)号：US06297037B1
公开(公告)日：2001-10-02
申请号：US08194664
申请日：1994-02-10
IPC分类号： C12N928
CPC分类号： C12N9/2417 , C11D3/386 , C12N15/75 , C12P19/14
摘要： Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
摘要翻译： 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。
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公开(公告)号：US5824532A
公开(公告)日：1998-10-20
申请号：US468220
申请日：1995-06-06
IPC分类号： C12N15/09 , C11D3/386 , C12N1/21 , C12N5/10 , C12N9/26 , C12N9/28 , C12N15/56 , C12N15/75 , C12P19/14 , C12N15/63
CPC分类号： C12N9/2417 , C11D3/386 , C12N15/75 , C12P19/14
摘要： Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
摘要翻译： 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。
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公开(公告)号：US5958739A
公开(公告)日：1999-09-28
申请号：US704706
申请日：1997-02-20
申请人： Colin Mitchinson , Carol Requadt , Traci Ropp , Leif P. Solheim , Christopher Ringer , Anthony Day
发明人： Colin Mitchinson , Carol Requadt , Traci Ropp , Leif P. Solheim , Christopher Ringer , Anthony Day
CPC分类号： C12N9/2417 , C12Y302/01001
摘要： Novel .alpha.-amylase enzymes are disclosed in which one or more asparagine residues are substituted with a different amino acid or deleted. The disclosed .alpha.-amylase enzymes show altered or improved low pH starch hydrolysis performance, stability and activity profiles.
摘要翻译： PCT No.PCT / US96 / 09089第 371日期1997年2月20日 102（e）1997年2月20日PCT PCT 1996年6月6日PCT公布。 公开号WO96 / 39528 日期：1996年12月19日公开了一种或多种天冬酰胺残基被不同氨基酸取代或缺失的新型α-淀粉酶。 公开的α-淀粉酶显示改变或改进的低pH淀粉水解性能，稳定性和活性谱。
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公开(公告)号：US5652127A
公开(公告)日：1997-07-29
申请号：US459984
申请日：1995-06-02
申请人： Colin Mitchinson , Leif P. Solheim
发明人： Colin Mitchinson , Leif P. Solheim
摘要： According to the invention a method is provided for liquefying starch comprising the steps of adding a sodium composition to the starch prior to or simultaneously with liquefying the starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Preferred sodium compositions comprise sodium chloride, sodium bicarbonate, sodium benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, sodium nitrate, sodium tartrate, sodium tetraborate, sodium propionate, sodium citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or a mixture thereof.
摘要翻译： 根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时将淀粉钠加入淀粉中; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。 优选的钠组合物包括氯化钠，碳酸氢钠，苯甲酸钠，硫酸钠，亚硫酸氢钠，抗坏血酸钠，乙酸钠，硝酸钠，酒石酸钠，四硼酸钠，丙酸钠，柠檬酸钠，琥珀酸钠，谷氨酸钠，柠檬酸三钠， 磷酸盐或其混合物。
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公开(公告)号：US5756714A
公开(公告)日：1998-05-26
申请号：US411038
申请日：1995-03-27
摘要： According to the invention a method is provided for liquefying starch comprising the steps of treating the starch prior to or simultaneously with liquefying the starch to inactivate and/or remove the enzyme inhibiting composition present in the starch and form treated starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Effective means to treat the starch include the addition of a phytate degrading enzyme and heat treatment, optionally followed by filtration or centrifugation, of granular starch or a starch solution.
摘要翻译： 根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时处理淀粉以灭活和/或除去淀粉中存在的酶抑制组合物并形成经处理的淀粉; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。 处理淀粉的有效方法包括加入植酸盐降解酶和热处理，任选地随后过滤或离心淀粉或淀粉溶液。
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公开(公告)号：US06184002B2
公开(公告)日：2001-02-06
申请号：US08952225
申请日：1998-01-27
申请人： Colin Mitchinson , Leif P. Solheim
发明人： Colin Mitchinson , Leif P. Solheim
IPC分类号： C12P1914
摘要： According to the invention a method is provided for liquefying starch comprising the steps of adding a sodium composition to the starch prior to or simultaneously with liquefying the starch; adding &agr;-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Preferred sodium compositions comprise sodium chloride, sodium bicarbonate, sodium benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, sodium nitrate, sodium tartrate, sodium tetraborate, sodium propionate, sodium citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or a mixture thereof.
摘要翻译： 根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时将淀粉钠加入淀粉中; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。 优选的钠组合物包括氯化钠，碳酸氢钠，苯甲酸钠，硫酸钠，亚硫酸氢钠，抗坏血酸钠，乙酸钠，硝酸钠，酒石酸钠，四硼酸钠，丙酸钠，柠檬酸钠，琥珀酸钠，谷氨酸钠，柠檬酸三钠， 磷酸盐或其混合物。
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公开(公告)号：US5736499A
公开(公告)日：1998-04-07
申请号：US468700
申请日：1995-06-06
CPC分类号： C12N9/2417 , C11D3/386
摘要： Novel .alpha.-amylase enzymes are disclosed in which one or more asparagine residues are substituted with a different amino acid or deleted. The disclosed .alpha.-amylase enzymes show altered or improved low pH starch hydrolysis performance, stability and activity profiles.
摘要翻译： 公开了一种或多种天冬酰胺残基被不同氨基酸取代或缺失的新型α-淀粉酶。 公开的α-淀粉酶显示改变或改进的低pH淀粉水解性能，稳定性和活性谱。
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公开(公告)号：US20140106408A1
公开(公告)日：2014-04-17
申请号：US14004881
申请日：2012-03-16
申请人： Colin Mitchinson , Steven S. Kim , Meredith K. Fujdala , Megan Y. Hsi , Keith D. Wing , William D. Hitz
发明人： Colin Mitchinson , Steven S. Kim , Meredith K. Fujdala , Megan Y. Hsi , Keith D. Wing , William D. Hitz
CPC分类号： C12P19/14 , C12N9/2437 , C12N9/2445 , C12N9/2485 , C12P7/14 , C12P19/02 , C12Y302/01021 , C12Y302/01032 , D21C5/00 , D21C5/005 , D21C11/0007 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase (GH) family 61/endoglucanase activity and/or a β-glucosidase polypeptide, methods for hydrolyzing biomass material, and methods for using such compositions.
摘要翻译： 本发明涉及可用于水解生物质的组合物，例如包含具有糖基水解酶（GH）家族61 /内切葡聚糖酶活性的多肽和/或葡糖苷酶多肽的组合物，水解生物质材料的方法和使用方法 这样的组合物。
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10.发明授权公开(公告)号：US08647850B2
公开(公告)日：2014-02-11
申请号：US12970992
申请日：2010-12-17
IPC分类号： C12P7/06
摘要： Methods are disclosed for the production of high concentrations of ethanol from biomass using Zymomonas as the ethanologen. Zymomonas is grown under conditions of low impeller agitation with high concentration of insoluble solids in a saccharification-fermentation mixture during a simultaneous saccharification and fermentation reaction for the production of high concentrations of ethanol.
摘要翻译： 公开了使用发酵单胞菌作为乙醇生物从生物质生产高浓度乙醇的方法。 在用于生产高浓度乙醇的同时糖化和发酵反应期间，发酵单体在糖化 - 发酵混合物中在低叶轮搅拌下以高浓度不溶性固体的条件下生长。
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