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公开(公告)号：US08229707B2
公开(公告)日：2012-07-24
申请号：US13175926
申请日：2011-07-04
CPC分类号： G01N15/1429 , G01N35/00712 , G01N35/0092
摘要： A method of extracting and analyzing a data set from a flow cytometer system of the preferred embodiment comprises the steps of (1) running a sample and saving all collected raw data, (2) viewing raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified data, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified data, and exporting the saved data as many times as necessary and/or desirable.
摘要翻译： 从优选实施例的流式细胞仪系统提取和分析数据集的方法包括以下步骤：（1）运行样本并保存所有收集的原始数据，（2）查看原始（或“未修改”）数据，（3 ）修改原始数据（例如，缩放和/或剔除原始数据），（4）查看和保存修改的数据，以及（5）导出保存的数据。 一旦样品运行并且所有收集的数据都被保存，用户可以重复修改原始数据，保存修改的数据，以及根据需要和/或期望导出保存的数据的步骤。
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公开(公告)号：US07996188B2
公开(公告)日：2011-08-09
申请号：US11466391
申请日：2006-08-22
CPC分类号： G01N15/1429 , G01N35/00712 , G01N35/0092
摘要： A method of extracting and analyzing a data set from a flow cytometer system of the preferred embodiment comprises the steps of (1) running a sample and saving all collected raw data, (2) viewing raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified data, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified data, and exporting the saved data as many times as necessary and/or desirable.
摘要翻译： 从优选实施例的流式细胞仪系统提取和分析数据集的方法包括以下步骤：（1）运行样本并保存所有收集的原始数据，（2）查看原始（或“未修改”）数据，（3 ）修改原始数据（例如，缩放和/或剔除原始数据），（4）查看和保存修改的数据，以及（5）导出保存的数据。 一旦样品运行并且所有收集的数据都被保存，用户可以重复修改原始数据，保存修改的数据，以及根据需要和/或期望导出保存的数据的步骤。
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公开(公告)号：US20080228444A1
公开(公告)日：2008-09-18
申请号：US11466391
申请日：2006-08-22
IPC分类号： G06F15/00
CPC分类号： G01N15/1429 , G01N35/00712 , G01N35/0092
摘要： The flow cytometer user interface of the preferred embodiment comprises the steps of (1) running the sample and saving all collected data, (2) viewing the raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified settings, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified settings, and exporting the saved data as many times as necessary and/or desirable without the need to run an additional sample.
摘要翻译： 优选实施例的流式细胞仪用户界面包括以下步骤：（1）运行样本并保存所有收集的数据，（2）查看原始（或“未修改”）数据，（3）修改原始数据（例如缩放 和/或剔除原始数据），（4）查看和保存修改的设置，以及（5）导出保存的数据。 一旦样品运行并且所有收集的数据都被保存，用户可以重复修改原始数据，保存修改后的设置以及根据需要和/或需要导出保存的数据的步骤，而不需要运行 额外的样品。
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公开(公告)号：US20080215297A1
公开(公告)日：2008-09-04
申请号：US11963384
申请日：2007-12-21
CPC分类号： G01N15/1459 , G01N2015/0092 , G01N2015/1477
摘要： The detection system of the first preferred embodiment includes a detector, having a wide dynamic range, that receives photonic inputs from the interrogation zone and produces an analog signal; and an analog-to-digital converter (ADC), having a high bit resolution, that is coupled to the detector and converts an analog signal to a digital signal. The digital signal includes an initial data set of the full dynamic range of the input signals from the flow cytometer sample. The method of extracting and analyzing data from a flow cytometer system of the first preferred embodiment preferably includes the steps of: collecting a full dynamic range of input signals from a flow cytometer sample; recognizing and annotating aggregate particle events; and storing an initial data set and an annotated data set of the full dynamic range of the input signals from the flow cytometer sample.
摘要翻译： 第一优选实施例的检测系统包括具有宽动态范围的检测器，其接收来自询问区的光子输入并产生模拟信号; 以及具有高比特分辨率的模数转换器（ADC），其耦合到检测器并将模拟信号转换为数字信号。 数字信号包括来自流式细胞仪样本的输入信号的完整动态范围的初始数据集。 从第一优选实施例的流式细胞仪系统中提取和分析数据的方法优选地包括以下步骤：从流式细胞仪样品收集输入信号的完整动态范围; 识别和注释聚集粒子事件; 并存储来自流式细胞仪样本的输入信号的完整动态范围的初始数据集和注释数据集。
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公开(公告)号：US20120004859A1
公开(公告)日：2012-01-05
申请号：US13175926
申请日：2011-07-04
IPC分类号： G06F19/00
CPC分类号： G01N15/1429 , G01N35/00712 , G01N35/0092
摘要： A method of extracting and analyzing a data set from a flow cytometer system of the preferred embodiment comprises the steps of (1) running a sample and saving all collected raw data, (2) viewing raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified data, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified data, and exporting the saved data as many times as necessary and/or desirable.
摘要翻译： 从优选实施例的流式细胞仪系统提取和分析数据集的方法包括以下步骤：（1）运行样本并保存所有收集的原始数据，（2）查看原始（或“未修改”）数据，（3 ）修改原始数据（例如，缩放和/或剔除原始数据），（4）查看和保存修改的数据，以及（5）导出保存的数据。 一旦样品运行并且所有收集的数据都被保存，用户可以重复修改原始数据，保存修改的数据，以及根据需要和/或期望导出保存的数据的步骤。
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公开(公告)号：US20100271620A1
公开(公告)日：2010-10-28
申请号：US12770341
申请日：2010-04-29
IPC分类号： G01N21/00
CPC分类号： G01N15/1459 , G01N2015/0092 , G01N2015/1477
摘要： The detection system of the first preferred embodiment includes a detector, having a wide dynamic range, that receives photonic inputs from the interrogation zone and produces an analog signal; and an analog-to-digital converter (ADC), having a high bit resolution, that is coupled to the detector and converts an analog signal to a digital signal. The digital signal includes an initial data set of the full dynamic range of the input signals from the flow cytometer sample. The method of extracting and analyzing data from a flow cytometer system of the first preferred embodiment preferably includes the steps of: collecting a full dynamic range of input signals from a flow cytometer sample; recognizing and annotating aggregate particle events; and storing an initial data set and an annotated data set of the full dynamic range of the input signals from the flow cytometer sample.
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公开(公告)号：US07739060B2
公开(公告)日：2010-06-15
申请号：US11963384
申请日：2007-12-21
CPC分类号： G01N15/1459 , G01N2015/0092 , G01N2015/1477
摘要： The detection system of the first preferred embodiment includes a detector, having a wide dynamic range, that receives photonic inputs from the interrogation zone and produces an analog signal; and an analog-to-digital converter (ADC), having a high bit resolution, that is coupled to the detector and converts an analog signal to a digital signal. The digital signal includes an initial data set of the full dynamic range of the input signals from the flow cytometer sample. The method of extracting and analyzing data from a flow cytometer system of the first preferred embodiment preferably includes the steps of: collecting a full dynamic range of input signals from a flow cytometer sample; recognizing and annotating aggregate particle events; and storing an initial data set and an annotated data set of the full dynamic range of the input signals from the flow cytometer sample.
摘要翻译： 第一优选实施例的检测系统包括具有宽动态范围的检测器，其接收来自询问区的光子输入并产生模拟信号; 以及具有高比特分辨率的模数转换器（ADC），其耦合到检测器并将模拟信号转换为数字信号。 数字信号包括来自流式细胞仪样本的输入信号的完整动态范围的初始数据集。 从第一优选实施例的流式细胞仪系统中提取和分析数据的方法优选地包括以下步骤：从流式细胞仪样品收集输入信号的完整动态范围; 识别和注释聚集粒子事件; 并存储来自流式细胞仪样本的输入信号的完整动态范围的初始数据集和注释数据集。
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公开(公告)号：US08229684B2
公开(公告)日：2012-07-24
申请号：US12770341
申请日：2010-04-29
CPC分类号： G01N15/1459 , G01N2015/0092 , G01N2015/1477
摘要： The detection system of the first preferred embodiment includes a detector, having a wide dynamic range, that receives photonic inputs from an interrogation zone and produces an analog signal; and an analog-to-digital converter (ADC), having a high bit resolution, that is coupled to the detector and converts an analog signal to a digital signal. The digital signal includes an initial data set of the full dynamic range of the input signals from the flow cytometer sample. The method of extracting and analyzing data from a flow cytometer system of the first preferred embodiment preferably includes the steps of: collecting a full dynamic range of input signals from a flow cytometer sample; recognizing and annotating aggregate particle events; and storing an initial data set and an annotated data set of the full dynamic range of the input signals from the flow cytometer sample.
摘要翻译： 第一优选实施例的检测系统包括具有宽动态范围的检测器，其接收来自询问区的光子输入并产生模拟信号; 以及具有高比特分辨率的模数转换器（ADC），其耦合到检测器并将模拟信号转换为数字信号。 数字信号包括来自流式细胞仪样本的输入信号的完整动态范围的初始数据集。 从第一优选实施例的流式细胞仪系统提取和分析数据的方法优选地包括以下步骤：从流式细胞仪样品收集输入信号的完整动态范围; 识别和注释聚集粒子事件; 并存储来自流式细胞仪样本的输入信号的完整动态范围的初始数据集和注释数据集。
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公开(公告)号：US09551600B2
公开(公告)日：2017-01-24
申请号：US13697739
申请日：2011-06-14
申请人： Grant C. Howes , Collin A. Rich
发明人： Grant C. Howes , Collin A. Rich
CPC分类号： G01F1/00 , G01F25/0007 , G01N15/14 , G01N35/00871 , G06F17/00 , G06F19/28
摘要： A system for creating a flow cytometer network includes: a flow cytometer with an interrogation zone and a fixed gain detection system that collects sample data from the interrogation zone; a flow cytometer data center that stores and manages sample-related data from the flow cytometer; and a network communication module that communicates sample-related data between the flow cytometer and the data center. The system may include a second flow cytometer and a second network communication module, where the first and second flow cytometers are calibrated to have substantially identical fixed gain settings. A method for creating a flow cytometer network includes: calibrating first and second flow cytometers with a calibration solution; collecting sample data with a fixed gain detection system; uploading data to a flow cytometer data center; retrieving data from the data center; and performing data analysis on the retrieved data.
摘要翻译： 用于产生流式细胞仪网络的系统包括：具有询问区的流式细胞仪和从询问区收集样本数据的固定增益检测系统; 流式细胞仪数据中心，用于存储和管理来自流式细胞仪的样品相关数据; 以及在流式细胞仪和数据中心之间传送样本相关数据的网络通信模块。 该系统可以包括第二流式细胞仪和第二网络通信模块，其中第一和第二流式细胞仪被校准以具有基本相同的固定增益设置。 创建流式细胞仪网络的方法包括：用校准溶液校准第一和第二流式细胞仪; 用固定增益检测系统收集样本数据; 将数据上传到流式细胞仪数据中心; 从数据中心检索数据; 并对所检索的数据执行数据分析。
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公开(公告)号：US08715573B2
公开(公告)日：2014-05-06
申请号：US11872676
申请日：2007-10-15
申请人： Jack T. Ball , Collin A. Rich
发明人： Jack T. Ball , Collin A. Rich
CPC分类号： G01N15/1404 , Y10T137/85978
摘要： The fluidic system 10 of the preferred embodiment includes a sheath pump 12 to pump sheath fluid 14 from a sheath container 16 through a sample port 34 into an interrogation zone 18 and a waste pump 20 to pump the sheath fluid 14 and a sample fluid 26 as waste fluid 22 from the interrogation zone 18 into a waste container 24, and a processor 30 to calculate a time window based on the flow rate of the sample fluid 26. Preferably the processor 30 also calculates a time window for the sample fluid to reach the interrogation zone 18 from the sample port 34 based on the flow rate of the sample fluid 26. The interrogation zone 18 functions to provide a location for the fluidic system 10 and an optical analysis system 32 of the flow cytometer to cooperatively facilitate the analysis of the sample fluid 26.
摘要翻译： 优选实施例的流体系统10包括护套泵12，其将护套流体14从鞘容器16通过样品端口34泵送到询问区域18和废水泵20，以将鞘液14和样品流体26泵送为 从询问区18到废物容器24的废液22以及基于样品流体26的流速计算时间窗的处理器30.优选地，处理器30还计算样品流体到达的时间窗口 询问区18基于样品流体26的流速从采样端口34询问。询问区18用于提供流体系统10的位置和流式细胞计数器的光学分析系统32，以便协调地促进对 样品流体26。
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