公开(公告)号：US20210277405A1

公开(公告)日：2021-09-09

申请号：US17205008

申请日：2021-03-18

申请人： Conagen Inc. ,  Sumitomo Chemical Co., Ltd.

发明人： Ilya Tikh ,  Sonya Clarkson ,  Brendon Dusel ,  Jacob Edward Vick ,  Xiaodan Yu ,  Bryan Salas-Santiago

IPC分类号： C12N15/77 ,  C12P1/04

摘要： The present invention relates to the creation of a control system within a host cell to limit or eliminate degradation of key specified products at certain times during fermentation and to redirect the metabolic flux of the cell toward higher production of same key specified products.

公开(公告)号：US20190024097A1

公开(公告)日：2019-01-24

申请号：US16036261

申请日：2018-07-16

申请人： Conagen, Inc.

发明人： Matthew de la Pena Mattozzi ,  Daniel Kim ,  Sonya Clarkson

IPC分类号： C12N15/70 ,  C12P21/00

摘要： The present invention relates to a Plasmid Addiction System for the stabilization of expression plasmids encoding proteins of interest. The invention uses a succinate cycle optimization to ensure the expression of plasmid(s) of interest. By ensuring that plasmids of interest contain genes necessary in the succinate cycle, the system ensures that the passage of the plasmid to daughters and therefore improves the efficiency of production and expression of genes and/or products of interest.

公开(公告)号：US20220275379A9

公开(公告)日：2022-09-01

申请号：US16036261

申请日：2018-07-16

申请人： Conagen, Inc.

发明人： Matthew de la Pena Mattozzi ,  Daniel Kim ,  Sonya Clarkson

IPC分类号： C12N15/70 ,  C12P21/00

摘要： The present invention relates to a Plasmid Addiction System for the stabilization of expression plasmids encoding proteins of interest. The invention uses a succinate cycle optimization to ensure the expression of plasmid(s) of interest. By ensuring that plasmids of interest contain genes necessary in the succinate cycle, the system ensures that the passage of the plasmid to daughters and therefore improves the efficiency of production and expression of genes and/or products of interest.

公开(公告)号：US11421239B2

公开(公告)日：2022-08-23

申请号：US16036261

申请日：2018-07-16

申请人： Conagen, Inc.

发明人： Matthew de la Pena Mattozzi ,  Daniel Kim ,  Sonya Clarkson

IPC分类号： C12N1/21 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02

摘要： The present invention relates to a Plasmid Addiction System for the stabilization of expression plasmids encoding proteins of interest. The invention uses a succinate cycle optimization to ensure the expression of plasmid(s) of interest. By ensuring that plasmids of interest contain genes necessary in the succinate cycle, the system ensures that the passage of the plasmid to daughters and therefore improves the efficiency of production and expression of genes and/or products of interest.