公开(公告)号：US10731187B2

公开(公告)日：2020-08-04

申请号：US15119825

申请日：2015-02-19

申请人： Covestro Deutschland AG

发明人： Gernot Jaeger ,  Jorgen Magnus ,  Amgad Salah Moussa ,  Guenter Olf ,  Giulio Lolli ,  Swantje Behnken ,  Jung-Won Youn ,  Mohammad Yalfani ,  Georg Sprenger

IPC分类号： C12P13/00 ,  C12P13/02 ,  C12P13/04 ,  C12N15/78 ,  C12N15/77 ,  C12N15/70 ,  C12N9/90 ,  C12N9/10 ,  C12N15/52

摘要： The invention provides a recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o-aminobenzoate biologically. The invention further provides a method for producing aniline, comprising the steps of: a) producing o-aminobenzoate by fermentation of a raw material comprising at least one fermentable carbon substrate using the recombinant microbial host cell of the capable of converting said raw material comprising at least one fermentable carbon substrate to o-aminobenzoate biologically, wherein said o-aminobenzoate comprises anthranilate anion, b) converting said o-aminobenzoate from said anthranilate anion to anthranilic acid by acid protonation, c) recovering said anthranilic acid by precipitation or by dissolving in an organic solvent, and d) converting said anthranilic acid to aniline by thermal decarboxylation in an organic solvent.

公开(公告)号：US20170152535A1

公开(公告)日：2017-06-01

申请号：US15119825

申请日：2015-02-19

申请人： Covestro Deutschland AG

发明人： Gernot Jaeger ,  Jorgen Magnus ,  Amgad Salah Moussa ,  Guenter Olf ,  Giulio Lolli ,  Swantje Behnken ,  Jung-Won Youn ,  Mohammad Yalfani ,  Georg Sprenger

IPC分类号： C12P13/00 ,  C12N15/70 ,  C12N9/90 ,  C12N15/78 ,  C12N9/10 ,  C12N15/52 ,  C12N15/77

CPC分类号： C12P13/001 ,  C12N9/1077 ,  C12N9/90 ,  C12N15/52 ,  C12N15/70 ,  C12N15/77 ,  C12N15/78 ,  C12P13/02 ,  C12P13/04 ,  C12Y204/02018 ,  C12Y504/99005

摘要： The invention provides a recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o-aminobenzoate biologically. The invention further provides a method for producing aniline, comprising the steps of: a) producing o-aminobenzoate by fermentation of a raw material comprising at least one fermentable carbon substrate using the recombinant microbial host cell of the capable of converting said raw material comprising at least one fermentable carbon substrate to o-aminobenzoate biologically, wherein said o-aminobenzoate comprises anthranilate anion, b) converting said o-aminobenzoate from said anthranilate anion to anthranilic acid by acid protonation, c) recovering said anthranilic acid by precipitation or by dissolving in an organic solvent, and d) converting said anthranilic acid to aniline by thermal decarboxylation in an organic solvent.