公开(公告)号：US20160151479A1

公开(公告)日：2016-06-02

申请号：US14900092

申请日：2014-06-30

申请人： Crucell Holland B.V.

发明人： Wenyu Dong ,  Pieter Rijken ,  Mike Ugwoke

IPC分类号： A61K39/145 ,  C12N7/00

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5258 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/16051 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16161 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/16261

摘要： Described are methods for preparing virosomes comprising the steps of: (a) providing an enveloped virus, and optionally inactivating the virus; (b) solubilizing the viral envelopes in a first solubilizing agent; (c) pre-solubilizing exogenous components in a second solubilizing agent; (d) adding the pre-solubilized exogenous components to the solubilized viral envelopes; and (e) reconstituting virosomal membranes by removing the solubilizing agent. According to the disclosure, the viral envelopes and the exogenous components are (pre-)solubilized at a temperature below 33° C.

公开(公告)号：US20160129106A1

公开(公告)日：2016-05-12

申请号：US14998169

申请日：2015-12-23

申请人： Crucell Holland B.V.

发明人： Wenyu Dong ,  Pieter Rijken ,  Mike Ugwoke

IPC分类号： A61K39/145 ,  C12N7/00

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5258 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/16051 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16161 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/16261

摘要： This disclosure relates to methods for preparing a virosome preparation comprising viral envelope protein of at least two, preferably at least three, more preferably at least four, different enveloped viruses, as well as to virosomal preparations obtained thereby. The disclosure also relates to the use of the virosomal preparations, e.g., as a vaccine.

摘要翻译： 本公开涉及制备包含至少两种，优选至少三种，更优选至少四种不同包膜病毒的病毒包膜蛋白的病毒颗粒制剂的方法，以及由此获得的病毒颗粒制剂。 本公开还涉及病毒颗粒制剂的用途，例如作为疫苗。

公开(公告)号：US20160199480A1

公开(公告)日：2016-07-14

申请号：US14392330

申请日：2014-06-30

申请人： Crucell Holland B.V.

发明人： Wenyu Dong ,  Pieter Rijken ,  Mike Ugwoke

IPC分类号： A61K39/145 ,  C12N7/00

CPC分类号： A61K39/12 ,  A61K9/1271 ,  A61K39/145 ,  A61K2039/5258 ,  A61K2039/55555 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251

摘要： Described are methods for preparing virosomes comprising the steps of: (a) providing an enveloped virus, and optionally inactivating the virus; (b) solubilizing the viral envelopes in a first solubilizing agent; (c) pre-solubilizing exogenous components in a second solubilizing agent; (d) adding the pre-solubilized exogenous components to the solubilized viral envelopes; and (e) reconstituting virosomal membranes by removing the solubilizing agent. According to the disclosure, the viral envelopes and the exogenous components are (pre-)solubilized at a temperature below 33° C.

摘要翻译： 描述了制备病毒体的方法，包括以下步骤：（a）提供包膜病毒，并任选地灭活病毒; （b）将病毒包膜溶解在第一增溶剂中; （c）在第二增溶剂中预先溶解外源成分; （d）将预溶解的外源成分加入到溶解的病毒包膜中; 和（e）通过除去增溶剂来重构病毒体膜。 根据公开内容，病毒包膜和外源成分在低于33℃的温度下被预先溶解。