公开(公告)号：US20180237817A1

公开(公告)日：2018-08-23

申请号：US15580121

申请日：2016-05-30

Applicant: CureVac AG

Inventor： Tilmann ROOS ,  Benyamin YAZDAN PANAH ,  Markus CONZELMANN ,  Andreas THESS ,  Dominik BUOB ,  Martin KUNZE ,  Veronika WAGNER

IPC: C12P19/34 ,  C12N11/08 ,  C12N9/10

CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N9/16 ,  C12N11/02 ,  C12N11/08 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/0705 ,  C12Y301/03033

Abstract: The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2′-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2′-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5′-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5′-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2′-O-methyltransferase.