公开(公告)号：US20040061852A1

公开(公告)日：2004-04-01

申请号：US10672114

申请日：2003-09-26

申请人： CyBio AG

发明人： Juergen Wulf ,  Andreas Fina ,  Michael Eberhard

IPC分类号： G01J001/10

CPC分类号： G01N21/278 ,  G01N21/6452

摘要： The invention is directed to a device for calibrating an optical detection channel for a two-dimensional, spatially dependent measurement of fluorescent or luminescent radiation in multi-specimen carriers. The object of the invention, to find a novel possibility for calibrating an optical detection channel for a two-dimensional, spatially dependent measurement of fluorescent or luminescent radiation in multi-specimen carriers permitting a highly accurate calibration of the spatial sensitivity distribution of the sensor array in the detection channel which is economical, can be repeated at any time and can be adapted to the intensity level of the measurement task, is met according to the invention by providing a plate-shaped housing which is manufactured in the shape and size of the multi-specimen carriers under examination and has, on its side facing the detection channel, a large-area rectangular window whose size is adapted to the surface of the multi-specimen carrier under examination, which surface is provided with wells, and there is a luminescent foil inside the housing which is arranged parallel to the window so as to cover its surface, and a power source and control units are provided in the housing for controlling the luminescent foil, so that the luminescent foil can be controlled for homogeneous emission of luminescent light through the window of the housing in different intensity levels.

摘要翻译： 本发明涉及用于校准用于多样本载体中的荧光或发光辐射的二维空间依赖性测量的光学检测通道的装置。 本发明的目的是找到用于校准用于在多个样本载体中进行二维空间依赖性测量荧光或发光辐射的光学检测通道的新颖可能性，允许高精度校准传感器阵列的空间灵敏度分布 在经济的检测通道中，可以在任何时间重复并且可以适应于测量任务的强度水平，根据本发明通过提供一种板形壳体来制造，该板状壳体的形状和尺寸 多个试样载体正在检查中，并且在其面向检测通道的一侧具有尺寸适合于被检查的多试样载体的表面的大面积矩形窗口，该表面设有孔，并且存在一个 壳体内的发光箔，其平行于窗口布置以覆盖其表面，电源和控制单元是专用的 在壳体内设置用于控制发光箔，从而可以控制发光箔以不同强度水平均匀地发射通过壳体的窗口的发光。

公开(公告)号：US20040022689A1

公开(公告)日：2004-02-05

申请号：US10630199

申请日：2003-07-30

申请人： CyBio AG

发明人： Juergen Wulf ,  Werner Pongratz ,  Andreas Fina ,  Michael Heil ,  Michael Jurtz

IPC分类号： G01N021/00

CPC分类号： G01N35/109 ,  G01N21/6428 ,  G01N21/6452 ,  G01N35/1011 ,  G01N35/1065 ,  G01N2021/6443 ,  G01N2021/6482 ,  G01N2035/0422

摘要： The invention is directed to a device for dispensing and observing the luminescence of individual specimens in multi-specimen arrangements, particularly for the examination of biological, chemical or cytobiological assays with high specimen throughput. It is the object of the invention to find a novel possibility for dispensing in multi-specimen arrangements (microplates) and for observing the luminescence of the individual specimens, particularly for examining biological assays with high specimen throughput, which permits an immediate observation of the course of luminescence while dispensing proceeds at the same time without being limited to a determined type of microplate. This object is met, according to the invention, in that the dispensing unit has at least one linear dispensing comb containing an even number of dispensing nozzles representing an integral divisor of the number of wells along one dimension of the microplate, the dispensing combs are arranged so as to be displaceable orthogonal to their longitudinal dimension, every dispensing comb is connected to a controllable pump for metering the amount of liquid to be dispensed without immersion in the wells of the microplate, and the CCD camera is oriented by a fast optical system to a large-area rectangular region of the underside of the microplate across from the dispensing unit, the surface being adapted to the dimension of the dispensing comb and to the area of the microplate covered by the dispensing comb, so that the elapsed time for the luminescence is measurable simultaneously while dispensing continues.

摘要翻译： 本发明涉及一种用于分配和观察多个样品装置中的各个样品的发光的装置，特别是用于以高样品生产量检查生物，化学或细胞生物学测定。 本发明的目的是找到一种新型的多样品布置（微孔板）分配和用于观察单个样品的发光的可能性，特别是用于检查具有高样品通量的生物测定法，其允许立即观察该过程 的发光，同时分配在同一时间进行，而不限于确定类型的微孔板。 根据本发明，符合本发明的目的在于，分配单元具有至少一个线性分配梳，其包含偶数个分配喷嘴，分配喷嘴表示沿着微孔板的一个维度的孔数的积分因子，分配梳被布置 为了能够与其纵向尺寸正交地移动，每个分配梳都连接到可控制的泵，用于计量待分配的液体量而不浸没在微板的孔中，并且CCD相机通过快速光学系统定向 微孔板下面的从分配单元的大面积矩形区域，该表面适于分配梳的尺寸和由分配梳覆盖的微板的面积，使得发光的经过时间 可同时测量，同时分配持续。