公开(公告)号：US20140216059A1

公开(公告)日：2014-08-07

申请号：US14174038

申请日：2014-02-06

申请人： CyBio AG

发明人： Thomas MOORE ,  Michael Kiehntopf

IPC分类号： C12M1/00

CPC分类号： C12M45/20 ,  B01L7/00 ,  B01L2200/141 ,  B01L2300/045 ,  B01L2300/049 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2300/1894 ,  B01L2400/0644

摘要： A cold box having a cooling receptacle filled with a dry protective gas in which is accommodated a rack loaded with tube-shaped vessels. The cooling receptacle is covered by a displaceable lid which is associated with a lid part and which is preferably formed by an outer lid and a rotatable inner lid integrated in the outer lid so that the cooling receptacle is completely covered in every position of the lid. At least one of the through-holes provided in the inner lid can be arranged over a respective tube-shaped vessel by a coordinated displacement of the outer lid and rotation of the inner lid so that the tube-shaped vessel located under the through-hole in alignment therewith can be filled with a sample through the through-hole by means of a commercially available pipette tip/dispensing needle of an automated pipetting device.

摘要翻译： 具有装有干燥保护气体的冷却容器的冷箱，容纳有装载有管状容器的齿条。 冷却容器被可移动的盖子覆盖，该盖子与盖部分相关联，该盖子优选地由外盖和可旋转的内盖形成，该内盖整合在外盖中，使得冷却容器在盖的每个位置被完全覆盖。 设置在内盖中的至少一个通孔可以通过外盖的协调位移和内盖的旋转而布置在相应的管状容器上，使得位于通孔下方的管状容器 与其对准可以通过自动移液装置的市售的移液管末端/分配针通过通孔填充样品。

公开(公告)号：US09084993B2

公开(公告)日：2015-07-21

申请号：US13622493

申请日：2012-09-19

申请人： CyBio AG

发明人： Uwe Naumann ,  Torsten Schoeppe ,  Heiko Oehme ,  Thomas Moore

IPC分类号： B01L3/02 ,  G01N35/10

CPC分类号： B01L3/0217 ,  B01L3/0275 ,  B01L2200/023 ,  G01N35/1074

摘要： A pipetting apparatus includes a pipetting head with a plurality of pipetting channels disposed in an arrangement pattern. Each pipetting channel includes a plunger and a cylinder. The plurality of pipetting channels includes at least two groups of pipetting channels with different diameters including a group of larger pipetting channels and a group of smaller pipetting channels. Each of the at least two groups of pipetting channels is disposed in an arrangement pattern in the pipetting head. Larger pipette tips communicate with the group of larger pipetting channels or smaller pipette tips communicate with the group of smaller pipetting channels.

摘要翻译： 移液装置包括具有以布置图案布置的多个移液通道的移液头。 每个移液通道包括柱塞和气缸。 多个移液通道包括至少两组具有不同直径的移液通道，包括一组较大的移液通道和一组较小的移液通道。 所述至少两组移液通道中的每一组以移动头中的布置图案布置。 较大的移液器吸头与较大的移液通道组进行通讯，或较小的移液管吸头与较小的移液通道组进行通讯。

公开(公告)号：US09416345B2

公开(公告)日：2016-08-16

申请号：US14174038

申请日：2014-02-06

申请人： CyBio AG

发明人： Thomas Moore ,  Michael Kiehntopf

IPC分类号： F25B21/02 ,  C12M1/00 ,  B01L7/00

CPC分类号： C12M45/20 ,  B01L7/00 ,  B01L2200/141 ,  B01L2300/045 ,  B01L2300/049 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2300/1894 ,  B01L2400/0644

摘要： A cold box having a cooling receptacle filled with a dry protective gas in which is accommodated a rack loaded with tube-shaped vessels. The cooling receptacle is covered by a movable lid which is associated with a lid part and which is preferably formed by an outer lid and a rotatable inner lid integrated in the outer lid so that the cooling receptacle is completely covered in every position of the lid. At least one of the through-holes provided in the inner lid can be arranged over a respective tube-shaped vessel by a coordinated movement of the outer lid and rotation of the inner lid so that the tube-shaped vessel located under the through-hole in alignment therewith can be filled with a sample through the through-hole by means of a commercially available pipette tip/dispensing needle of an automated pipetting device.

摘要翻译： 具有装有干燥保护气体的冷却容器的冷箱，容纳有装载有管状容器的齿条。 冷却容器由与盖部分相关联的可移动盖子覆盖，该盖子优选地由外盖和可旋转的内盖形成，该内盖整合在外盖中，使得冷却容器在盖的每个位置被完全覆盖。 设置在内盖中的通孔中的至少一个可以通过外盖的协调运动和内盖的旋转而布置在相应的管状容器上，使得位于通孔下方的管状容器 与其对准可以通过自动移液装置的市售的移液管末端/分配针通过通孔填充样品。

公开(公告)号：US20040232162A1

公开(公告)日：2004-11-25

申请号：US10722366

申请日：2003-11-25

申请人： CyBio AG

发明人： Peter Zimmermann ,  Torsten Rausch ,  Gerd Heibe ,  Hartmut Koberich ,  Simon Renard ,  Thomas Moore ,  Wolfgang Kramer

IPC分类号： B67D005/08

CPC分类号： B01L3/021 ,  B01L2200/148 ,  B01L2300/0861 ,  B01L2400/0487 ,  B01L2400/06 ,  G01F11/28 ,  G01F13/006 ,  G01F25/0092 ,  G01N35/00594 ,  G01N35/00693 ,  G01N35/1016 ,  G01N35/1065 ,  G01N35/1072 ,  G01N2035/1039 ,  Y10T436/2575

摘要： Multi-channel metering apparatus with automatic calibration, where the individual dispensing channels 1, the micro-valves 4 of which are connected with the outlets of a distributor 8, 13 or 15 whose inlet is filled with fluid by a vessel 9, 14 or 17 thus connected, are able to be calibrated in relation to each other in that between the vessel 9, 14 or 17 and the inlet of the distributor 8, 13 or 15 a flow sensor 10 is arranged which for calibration during delivery of the fluid via a respective nozzle 2 of a respective dispensing channel 1 records the flow and generates signals which are respectively assigned to a defined opening time of a micro-valve 4 and a specific dispensing channel 1 and thus stored allows for individual control of the micro-valves 4 in order to equalize the tolerances of the dispensing channels 1.

摘要翻译： 具有自动校准的多通道计量装置，其中各个分配通道1，其微型阀4与分配器8,13或15的出口连接，分配器8,13或15的入口由容器9,14或17填充流体 因此，能够相对于彼此校准，就是在容器9,14或17与分配器8,13或15的入口之间进行校准，设置流量传感器10，用于在流体经由 相应的分配通道1的相应喷嘴2记录流量并产生分别被分配到微阀4和特定分配通道1的限定打开时间的信号，并且因此存储允许微型阀4的单独控制 以便均衡分配通道1的公差。

公开(公告)号：US20040061852A1

公开(公告)日：2004-04-01

申请号：US10672114

申请日：2003-09-26

申请人： CyBio AG

发明人： Juergen Wulf ,  Andreas Fina ,  Michael Eberhard

IPC分类号： G01J001/10

CPC分类号： G01N21/278 ,  G01N21/6452

摘要： The invention is directed to a device for calibrating an optical detection channel for a two-dimensional, spatially dependent measurement of fluorescent or luminescent radiation in multi-specimen carriers. The object of the invention, to find a novel possibility for calibrating an optical detection channel for a two-dimensional, spatially dependent measurement of fluorescent or luminescent radiation in multi-specimen carriers permitting a highly accurate calibration of the spatial sensitivity distribution of the sensor array in the detection channel which is economical, can be repeated at any time and can be adapted to the intensity level of the measurement task, is met according to the invention by providing a plate-shaped housing which is manufactured in the shape and size of the multi-specimen carriers under examination and has, on its side facing the detection channel, a large-area rectangular window whose size is adapted to the surface of the multi-specimen carrier under examination, which surface is provided with wells, and there is a luminescent foil inside the housing which is arranged parallel to the window so as to cover its surface, and a power source and control units are provided in the housing for controlling the luminescent foil, so that the luminescent foil can be controlled for homogeneous emission of luminescent light through the window of the housing in different intensity levels.

摘要翻译： 本发明涉及用于校准用于多样本载体中的荧光或发光辐射的二维空间依赖性测量的光学检测通道的装置。 本发明的目的是找到用于校准用于在多个样本载体中进行二维空间依赖性测量荧光或发光辐射的光学检测通道的新颖可能性，允许高精度校准传感器阵列的空间灵敏度分布 在经济的检测通道中，可以在任何时间重复并且可以适应于测量任务的强度水平，根据本发明通过提供一种板形壳体来制造，该板状壳体的形状和尺寸 多个试样载体正在检查中，并且在其面向检测通道的一侧具有尺寸适合于被检查的多试样载体的表面的大面积矩形窗口，该表面设有孔，并且存在一个 壳体内的发光箔，其平行于窗口布置以覆盖其表面，电源和控制单元是专用的 在壳体内设置用于控制发光箔，从而可以控制发光箔以不同强度水平均匀地发射通过壳体的窗口的发光。

公开(公告)号：US20140377147A1

公开(公告)日：2014-12-25

申请号：US14310160

申请日：2014-06-20

申请人： CyBio AG

发明人： Guenther SAMMLER ,  Mario BUESCHEL ,  Baerbel TAUTKUS ,  Heidrun RHODE ,  Sindy WENDLER

IPC分类号： B01L3/02 ,  B01D15/22

CPC分类号： B01L3/0275 ,  B01D15/22 ,  B01L2300/0681 ,  B01L2400/0605

摘要： Chromatography pipette tip having a first vessel and a second vessel which is open at two opposite ends and which, in each instance, has a bottom orifice via which a sample liquid can either be sucked in or expelled. The two vessels are fluidically and sealably connected to one another. A reaction matrix is arranged in one of the two vessels, through which reaction matrix the sample liquid is pushed by means of a pipettor to which the chromatography pipette tip is connected when used as intended. The sample liquid is drawn in and dispensed in opposite directions by the chromatography pipette tip and flows through the reaction matrix only in one direction.

摘要翻译： 具有第一容器和第二容器的色谱吸头，该第二容器在两个相对的端部是开放的，并且在每个实例中都具有一个底部孔口，样品液体可以通过该底部孔口被吸入或排出。 两个容器在流体上和密封地相互连接。 将反应基质排列在两个容器中的一个中，通过移液器将样品液体通过反应基质通过反应基质，当按照预期使用时，色谱移液管尖端连接到该移液管上。 样品液体通过色谱吸头提取并以相反方向分配，并且仅在一个方向上流过反应基质。

公开(公告)号：US20130068041A1

公开(公告)日：2013-03-21

申请号：US13622493

申请日：2012-09-19

申请人： CyBio AG

发明人： Uwe Naumann ,  Torsten Schoeppe ,  Heiko Oehme ,  Thomas Moore

IPC分类号： B01L3/02

CPC分类号： B01L3/0217 ,  B01L3/0275 ,  B01L2200/023 ,  G01N35/1074

摘要： A pipetting apparatus including a pipetting head with a plurality of pipetting channels disposed in an arrangement pattern. Each pipetting channel includes a plunger and a cylinder. The plurality of pipetting channels includes at least two groups of pipetting channels with different diameters including a group of larger pipetting channels and a group of smaller pipetting channels. Each of the at least two groups of pipetting channels is disposed in an arrangement pattern in the pipetting head. Larger pipette tips communicate with the group of larger pipetting channels or smaller pipette tips communicate with the group of smaller pipetting channels.

摘要翻译： 一种移液装置，包括具有以排列图案设置的多个移液通道的移液头。 每个移液通道包括柱塞和气缸。 多个移液通道包括至少两组具有不同直径的移液通道，包括一组较大的移液通道和一组较小的移液通道。 所述至少两组移液通道中的每一组以移动头中的布置图案布置。 较大的移液器吸头与较大的移液通道组进行通讯，或较小的移液管吸头与较小的移液通道组进行通讯。

公开(公告)号：US20040022689A1

公开(公告)日：2004-02-05

申请号：US10630199

申请日：2003-07-30

申请人： CyBio AG

发明人： Juergen Wulf ,  Werner Pongratz ,  Andreas Fina ,  Michael Heil ,  Michael Jurtz

IPC分类号： G01N021/00

CPC分类号： G01N35/109 ,  G01N21/6428 ,  G01N21/6452 ,  G01N35/1011 ,  G01N35/1065 ,  G01N2021/6443 ,  G01N2021/6482 ,  G01N2035/0422

摘要： The invention is directed to a device for dispensing and observing the luminescence of individual specimens in multi-specimen arrangements, particularly for the examination of biological, chemical or cytobiological assays with high specimen throughput. It is the object of the invention to find a novel possibility for dispensing in multi-specimen arrangements (microplates) and for observing the luminescence of the individual specimens, particularly for examining biological assays with high specimen throughput, which permits an immediate observation of the course of luminescence while dispensing proceeds at the same time without being limited to a determined type of microplate. This object is met, according to the invention, in that the dispensing unit has at least one linear dispensing comb containing an even number of dispensing nozzles representing an integral divisor of the number of wells along one dimension of the microplate, the dispensing combs are arranged so as to be displaceable orthogonal to their longitudinal dimension, every dispensing comb is connected to a controllable pump for metering the amount of liquid to be dispensed without immersion in the wells of the microplate, and the CCD camera is oriented by a fast optical system to a large-area rectangular region of the underside of the microplate across from the dispensing unit, the surface being adapted to the dimension of the dispensing comb and to the area of the microplate covered by the dispensing comb, so that the elapsed time for the luminescence is measurable simultaneously while dispensing continues.

摘要翻译： 本发明涉及一种用于分配和观察多个样品装置中的各个样品的发光的装置，特别是用于以高样品生产量检查生物，化学或细胞生物学测定。 本发明的目的是找到一种新型的多样品布置（微孔板）分配和用于观察单个样品的发光的可能性，特别是用于检查具有高样品通量的生物测定法，其允许立即观察该过程 的发光，同时分配在同一时间进行，而不限于确定类型的微孔板。 根据本发明，符合本发明的目的在于，分配单元具有至少一个线性分配梳，其包含偶数个分配喷嘴，分配喷嘴表示沿着微孔板的一个维度的孔数的积分因子，分配梳被布置 为了能够与其纵向尺寸正交地移动，每个分配梳都连接到可控制的泵，用于计量待分配的液体量而不浸没在微板的孔中，并且CCD相机通过快速光学系统定向 微孔板下面的从分配单元的大面积矩形区域，该表面适于分配梳的尺寸和由分配梳覆盖的微板的面积，使得发光的经过时间 可同时测量，同时分配持续。