公开(公告)号：US11623941B2

公开(公告)日：2023-04-11

申请号：US16884475

申请日：2020-05-27

Applicant: Cytiva BioProcess R&D AB

Inventor： Annika Kristina Forss ,  Gustav Jose Rodrigo ,  Tomas Bjorkman ,  Jesper Ulf Hansson ,  Mats Ander

IPC: C07K1/22 ,  B01D15/08 ,  C07K16/12 ,  C07K17/10 ,  B01J20/286 ,  C07K14/31 ,  C07K16/06 ,  B01D15/00 ,  B01J20/32

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.

公开(公告)号：US20230257417A1

公开(公告)日：2023-08-17

申请号：US18181974

申请日：2023-03-10

Applicant: Cytiva BioProcess R&D AB

Inventor： Annika Kristina Forss ,  Gustav Jose Rodrigo ,  Tomas Bjorkman ,  Jesper Ulf Hansson ,  Mats Ander

IPC: C07K1/22 ,  C07K16/12 ,  C07K17/10 ,  B01J20/286 ,  C07K14/31 ,  C07K16/06 ,  B01D15/00 ,  B01J20/32

CPC classification number: C07K1/22 ,  C07K16/1271 ,  C07K17/10 ,  B01J20/286 ,  C07K14/31 ,  C07K16/065 ,  B01D15/00 ,  B01J20/3274 ,  B01J20/3212 ,  B01J2220/52

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support: b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing said separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA).