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公开(公告)号：US20180208945A1
公开(公告)日：2018-07-26
申请号：US15746479
申请日：2016-07-28
申请人： DANISCO US INC.
发明人： Kai BAO , Susan Mampusti MADRID , Brian F. SCHMIDT
CPC分类号： C12N15/902 , C12N9/22 , C12N15/102 , C12N15/11 , C12N15/80 , C12N2310/20 , C12N2800/80
摘要： Compositions and methods are provided for genome editing at a target site in the genome of a filamentous fungal cell. The methods and compositions disclosed are drawn to a guide polynucleotide/Cas endonuclease system and donor polynucleotides with shorter homology arms (i.e., less than 500 bps) to a genomic locus of the fungal cell.
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公开(公告)号：US20190032065A1
公开(公告)日：2019-01-31
申请号：US15108106
申请日：2014-12-05
申请人： DANISCO US INC.
摘要： The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Gram positive microorganisms, such as Bacillus species, having a genetic alteration that reduces the expression of a gene in the pdh operon and results in enhanced expression of a protein of interest.
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公开(公告)号：US20170369537A1
公开(公告)日：2017-12-28
申请号：US15533487
申请日：2015-12-11
申请人： DANISCO US INC.
摘要： The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Grampositive microorganisms, such as members of the Bacillus genus having a genetic alteration that delays, reduces, or blocks the expression or activation of genes for sporulation, thereby resulting in enhanced expression of a protein of interest. The genetic alteration is one that reduces expression of a kinA gene, a phrA gene or a phrE gene.
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公开(公告)号：US20170362628A1
公开(公告)日：2017-12-21
申请号：US15529948
申请日：2015-12-03
申请人： DANISCO US INC.
发明人： Cristina BONGIORNI , Rodante GONZALES CAGUIAT , Carol Marie FIORESI , Brian F. SCHMIDT , Anita VAN KIMMENADE , Chao ZHU
CPC分类号： C12P21/02 , C07K14/32 , C12N9/2417 , C12N9/54 , C12N9/88 , C12N15/75 , C12Y302/01001 , C12Y304/21062 , C12Y402/02002
摘要： The present invention relates in general to bacterial cells having genetic alterations that result in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include altered Gram positive microorganismshaving one or more a genetic alterations that reduce the expression of a gene in the sin operon, thereby resulting in the enhanced expression of one or more proteins of interest.
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公开(公告)号：US20240279627A1
公开(公告)日：2024-08-22
申请号：US17270220
申请日：2019-08-19
申请人： DANISCO US INC
发明人： Despina BOUGIOUKOU , Robert DICOSIMO , Angel Elizabeth Grace D'OLIVIERA , Jonathan David PETERSON , Jahnavi Chandra PRASAD , Brian F. SCHMIDT , Joshua Joyner SIMS
CPC分类号： C12N9/20 , C11D3/38627 , C12Y301/01003
摘要： The present disclosure provides lipolytic enzyme variants having one or more modifications as compared to a parent lipolytic enzyme. Specifically, the present disclosure provides one or more lipolytic enzyme variants that has at least one improved performance when compared to one or more reference lipolytic enzymes. In addition, the present disclosure provides compositions comprising a lipolytic enzyme variant of the disclosure. The present disclosure also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the disclosure.
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公开(公告)号：US20160319288A1
公开(公告)日：2016-11-03
申请号：US15108074
申请日：2014-12-08
申请人： DANISCO US INC.
IPC分类号： C12N15/75
摘要： The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Gram positive microorganisms, such as Bacillus species, having a genetic alteration that modifies activity of a protein encoded by the ykf operon and results in enhanced expression of a protein of interest.
摘要翻译： 本发明一般涉及具有导致目的蛋白质表达增加的遗传改变的细菌细胞和制备和使用这些细胞的方法。 本发明的方面包括具有改变由ykf操纵子编码的蛋白质的活性的遗传改变的革兰氏阳性微生物，例如芽孢杆菌属物种，并导致目的蛋白质的表达增强。
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