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1.发明授权Three-dimensional structure of isoprene synthase and its use thereof for generating variants 有权异戊二烯合酶的三维结构及其用于产生变体的用途公开(公告)号：US09175313B2
公开(公告)日：2015-11-03
申请号：US13888265
申请日：2013-05-06
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Richard R. Bott , Jeffrey V. Miller , Christopher L. Rife , Derek H. Wells
CPC classification number: C12P5/007 , C07K2299/00 , C12N9/88 , C12Y402/03027
Abstract: The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
Abstract translation: 本发明提供了三叶虫异戊二烯合酶和异戊二烯异戊二烯合酶的三维结构。 本发明还提供使用三维结构设计异戊二烯合酶的方法，所述异戊二烯合酶具有改进的微生物宿主细胞中异戊二烯生产增加的活性。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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2.发明授权Identification of isoprene synthase variants with improved properties for the production of isoprene 有权鉴定异戊二烯合成酶变体具有改进的生产异戊二烯的性质公开(公告)号：US09163263B2
公开(公告)日：2015-10-20
申请号：US13801971
申请日：2013-03-13
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , David A. Estell , Jeffrey V. Miller , James Ngai , Christopher L. Rife , Derek H. Wells
CPC classification number: C12P5/007 , C12N9/88 , C12Y402/03027
Abstract: The invention provides for compositions and methods for producing isoprene using isoprene synthase variants with improved properties.
Abstract translation: 本发明提供使用具有改进性能的异戊二烯合酶变体产生异戊二烯的组合物和方法。
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3.发明申请公开(公告)号：US20200095614A1
公开(公告)日：2020-03-26
申请号：US16469721
申请日：2017-12-15
Applicant: DANISCO US INC.
Inventor： Jeffrey V. Miller , Bradley Roger Kelemen , Pim Van Der Kley , Igor Nikolaev , Su Yin Marina Chow , Monica Tse , Jing Ge , Zhongmei Tang , Kirstin Yasuko Nose Crotty
Abstract: Described are compositions and methods relating the use of α-glucosidase to increase the production of ethanol from a corn fiber product in a starch hydrolysis process.
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