公开(公告)号：US10077286B2

公开(公告)日：2018-09-18

申请号：US14395449

申请日：2013-04-19

申请人： DENKA SEIKEN CO., LTD.

发明人： Motoko Ohta

IPC分类号： C07K1/14 ,  G01N33/92 ,  G01N33/68 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/61

CPC分类号： C07K1/14 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/485 ,  C12Q1/61 ,  G01N33/6893 ,  G01N33/92 ,  G01N2800/32 ,  G01N2800/7085

摘要： Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.

公开(公告)号：US20150132834A1

公开(公告)日：2015-05-14

申请号：US14395449

申请日：2013-04-19

申请人： DENKA SEIKEN CO., LTD.

发明人： Motoko Ohta

IPC分类号： C07K1/14

CPC分类号： C07K1/14 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/485 ,  C12Q1/61 ,  G01N33/6893 ,  G01N33/92 ,  G01N2800/32 ,  G01N2800/7085

摘要： Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.

摘要翻译： 公开了一种选择性地除去低密度脂蛋白以外的脂蛋白中的甘油三酯的方法，该方法允许使用自动分析仪等以极好的简便性，特异性和精确度提供直接和差异定量样品中的LDL-TG的方法，而没有 进行诸如离心或电泳之类的预处理的费力操作。 在低密度脂蛋白以外的脂蛋白中消除甘油三酸酯的方法包括使脂蛋白脂肪酶，胆固醇酯酶，甘油激酶和甘油-3-磷酸氧化酶在作用于低密度脂蛋白以外的脂蛋白的表面活性剂存在下作用于样品， /或具有低密度脂蛋白保护作用的表面活性剂，并消除由此产生的过氧化氢。